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Platycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses.
Int Immunopharmacol. 2008 Aug; 8(8):1143-50.II

Abstract

Platycodin D2 (PD2), a saponin isolated from the root of Platycodon grandiflorum, was evaluated for its haemolytic activity and adjuvant potential on the cellular and humoral immune responses to ovalbumin (OVA) in ICR mice. The HD(50) values of PD2 and Quil A were 18.57+/-1.37 and 5.76+/-0.23 microg/ml against 0.5% rabbit red blood cell, respectively, implicating the less haemolytic activity for PD2 than Quil A. ICR mice were immunized subcutaneously with OVA 100 microg alone or with OVA 100 microg dissolved in saline containing Alum (200 microg), or PD2 (25, 50, 75 or 100 microg) on Day 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS-) and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. PD2 significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice (P<0.05, P<0.01 or P<0.001). OVA-specific IgG, IgG1, IgG2a, and IgG2b antibody titers in serum were also significantly enhanced by PD2 compared with OVA control group (P<0.05, P<0.01 or P<0.001). Further, the effects of PD2 on mRNA expression of cytokines and transcription factors in Con A-stimulated mice splenocytes were evaluated by RT-PCR analysis. PD2 significantly promoted the mRNA expression of cytokines IL-2, IFN-gamma, IL-4, and IL-10 and transcription factors T-bet and GATA-3 (P<0.05, P<0.01 or P<0.001). In conclusion, the results suggest that PD2 could be safely used as adjuvant eliciting Th1 and Th2 immune responses.

Authors+Show Affiliations

College of Animal Sciences, Zhejiang University, Hangzhou 310029, PR China; School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18550019

Citation

Xie, Yong, et al. "Platycodin D2 Is a Potential Less Hemolytic Saponin Adjuvant Eliciting Th1 and Th2 Immune Responses." International Immunopharmacology, vol. 8, no. 8, 2008, pp. 1143-50.
Xie Y, Deng W, Sun H, et al. Platycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses. Int Immunopharmacol. 2008;8(8):1143-50.
Xie, Y., Deng, W., Sun, H., & Li, D. (2008). Platycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses. International Immunopharmacology, 8(8), 1143-50. https://doi.org/10.1016/j.intimp.2008.04.006
Xie Y, et al. Platycodin D2 Is a Potential Less Hemolytic Saponin Adjuvant Eliciting Th1 and Th2 Immune Responses. Int Immunopharmacol. 2008;8(8):1143-50. PubMed PMID: 18550019.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Platycodin D2 is a potential less hemolytic saponin adjuvant eliciting Th1 and Th2 immune responses. AU - Xie,Yong, AU - Deng,Wen, AU - Sun,Hongxiang, AU - Li,Duo, Y1 - 2008/05/09/ PY - 2008/03/10/received PY - 2008/04/13/revised PY - 2008/04/15/accepted PY - 2008/6/14/pubmed PY - 2008/9/17/medline PY - 2008/6/14/entrez SP - 1143 EP - 50 JF - International immunopharmacology JO - Int Immunopharmacol VL - 8 IS - 8 N2 - Platycodin D2 (PD2), a saponin isolated from the root of Platycodon grandiflorum, was evaluated for its haemolytic activity and adjuvant potential on the cellular and humoral immune responses to ovalbumin (OVA) in ICR mice. The HD(50) values of PD2 and Quil A were 18.57+/-1.37 and 5.76+/-0.23 microg/ml against 0.5% rabbit red blood cell, respectively, implicating the less haemolytic activity for PD2 than Quil A. ICR mice were immunized subcutaneously with OVA 100 microg alone or with OVA 100 microg dissolved in saline containing Alum (200 microg), or PD2 (25, 50, 75 or 100 microg) on Day 1 and 15. Two weeks later (Day 28), concanavalin A (Con A)-, lipopolysaccharide (LPS-) and OVA-stimulated splenocyte proliferation and OVA-specific antibodies in serum were measured. PD2 significantly enhanced the Con A-, LPS-, and OVA-induced splenocyte proliferation in the OVA-immunized mice (P<0.05, P<0.01 or P<0.001). OVA-specific IgG, IgG1, IgG2a, and IgG2b antibody titers in serum were also significantly enhanced by PD2 compared with OVA control group (P<0.05, P<0.01 or P<0.001). Further, the effects of PD2 on mRNA expression of cytokines and transcription factors in Con A-stimulated mice splenocytes were evaluated by RT-PCR analysis. PD2 significantly promoted the mRNA expression of cytokines IL-2, IFN-gamma, IL-4, and IL-10 and transcription factors T-bet and GATA-3 (P<0.05, P<0.01 or P<0.001). In conclusion, the results suggest that PD2 could be safely used as adjuvant eliciting Th1 and Th2 immune responses. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/18550019/Platycodin_D2_is_a_potential_less_hemolytic_saponin_adjuvant_eliciting_Th1_and_Th2_immune_responses_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(08)00132-X DB - PRIME DP - Unbound Medicine ER -