Tags

Type your tag names separated by a space and hit enter

A multiple protocol to improve diagnosis and isolation of Shiga toxin-producing Escherichia coli from human stool specimens.
Diagn Microbiol Infect Dis. 2008 Sep; 62(1):7-10.DM

Abstract

Many infections caused by Shiga toxin-producing Escherichia coli (STEC) are undiagnosed, particularly non-O157 STEC. We evaluated the use of a multiple protocol approach to improve diagnosis, isolation, and characterization of STEC strains. Among 18 presumptive STEC-positive stool samples received by the INOVA Fairfax Hospital, Falls Church, VA, in 2006, 16 were Shiga toxin positive. From these 16 stool samples, 8 O157:H7 and 5 non-O157 STEC were isolated by plating onto sorbitol MacConkey (SMAC) agar. The remaining 5 stool samples that did not yield colonies on SMAC agar plates were enriched. All enriched samples were Shiga toxin positive, and 2 O157:H7 and 1 non-O157 STEC were subsequently isolated. The 2 remaining enriched samples did not yield isolates; however, based on polymerase chain reaction (PCR) analysis, both samples contained STEC genes. Based on PCR analysis of non-O157 strains, 3 strain types were identified. Samples from 3 patients, received within 2 days of one another, had a similar gene profile-eae and stx(1) negative and stx(2) positive-suggesting that these patients were likely infected with the same strain. Our results indicate that a multiple protocol approach is necessary to reliably diagnose and isolate STEC strains, and that PCR profiling of strains could allow for more rapid identification of outbreaks.

Authors+Show Affiliations

Environmental Microbial Safety Laboratory, U.S. Department of Agriculture, Animal and Natural Resources Institute, Agricultural Research Service, Beltsville, MD, USA. dan.shelton@ars.usda.govNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

18550318

Citation

Shelton, Daniel R., et al. "A Multiple Protocol to Improve Diagnosis and Isolation of Shiga Toxin-producing Escherichia Coli From Human Stool Specimens." Diagnostic Microbiology and Infectious Disease, vol. 62, no. 1, 2008, pp. 7-10.
Shelton DR, Karns JS, Park CH. A multiple protocol to improve diagnosis and isolation of Shiga toxin-producing Escherichia coli from human stool specimens. Diagn Microbiol Infect Dis. 2008;62(1):7-10.
Shelton, D. R., Karns, J. S., & Park, C. H. (2008). A multiple protocol to improve diagnosis and isolation of Shiga toxin-producing Escherichia coli from human stool specimens. Diagnostic Microbiology and Infectious Disease, 62(1), 7-10. https://doi.org/10.1016/j.diagmicrobio.2008.05.001
Shelton DR, Karns JS, Park CH. A Multiple Protocol to Improve Diagnosis and Isolation of Shiga Toxin-producing Escherichia Coli From Human Stool Specimens. Diagn Microbiol Infect Dis. 2008;62(1):7-10. PubMed PMID: 18550318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A multiple protocol to improve diagnosis and isolation of Shiga toxin-producing Escherichia coli from human stool specimens. AU - Shelton,Daniel R, AU - Karns,Jeffrey S, AU - Park,Choong H, Y1 - 2008/06/11/ PY - 2007/12/03/received PY - 2008/04/25/revised PY - 2008/05/01/accepted PY - 2008/6/14/pubmed PY - 2008/10/16/medline PY - 2008/6/14/entrez SP - 7 EP - 10 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 62 IS - 1 N2 - Many infections caused by Shiga toxin-producing Escherichia coli (STEC) are undiagnosed, particularly non-O157 STEC. We evaluated the use of a multiple protocol approach to improve diagnosis, isolation, and characterization of STEC strains. Among 18 presumptive STEC-positive stool samples received by the INOVA Fairfax Hospital, Falls Church, VA, in 2006, 16 were Shiga toxin positive. From these 16 stool samples, 8 O157:H7 and 5 non-O157 STEC were isolated by plating onto sorbitol MacConkey (SMAC) agar. The remaining 5 stool samples that did not yield colonies on SMAC agar plates were enriched. All enriched samples were Shiga toxin positive, and 2 O157:H7 and 1 non-O157 STEC were subsequently isolated. The 2 remaining enriched samples did not yield isolates; however, based on polymerase chain reaction (PCR) analysis, both samples contained STEC genes. Based on PCR analysis of non-O157 strains, 3 strain types were identified. Samples from 3 patients, received within 2 days of one another, had a similar gene profile-eae and stx(1) negative and stx(2) positive-suggesting that these patients were likely infected with the same strain. Our results indicate that a multiple protocol approach is necessary to reliably diagnose and isolate STEC strains, and that PCR profiling of strains could allow for more rapid identification of outbreaks. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/18550318/A_multiple_protocol_to_improve_diagnosis_and_isolation_of_Shiga_toxin_producing_Escherichia_coli_from_human_stool_specimens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(08)00240-X DB - PRIME DP - Unbound Medicine ER -