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A simple one-step real-time RT-PCR for diagnosis of dengue virus infection.
J Med Virol. 2008 Aug; 80(8):1426-33.JM

Abstract

Dengue is the most important arbovirus disease in tropical and sub-tropical countries, and can be caused by infection with any of the four-dengue virus (DENV) serotypes. Infection with DENV can lead to a broad clinical spectrum, ranging from sub-clinical infection or an influenza-like disease known as dengue fever (DF) to a severe, sometimes fatal, disease characterized by hemorrhage and plasma leakage that can lead to shock, known as dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). The diagnosis of dengue is routinely accomplished by serologic assays, such as IgM and IgG ELISAs, as well as HI tests, analyzing serum samples obtained from patients with at least 7 days of symptoms onset. These tests cannot be used for diagnosis during the early symptomatic phase. In addition, antibodies against dengue are broad reactive with other flaviviruses. Therefore, a specific diagnostic method for acute DENV infection is of great interest. In that sense, the real-time RT-PCR has become an important tool that can be used for early and specific detection of dengue virus genome in human serum samples. This study describes a simple, specific, and sensitive real-time RT-PCR for early diagnosis of dengue virus infection.

Authors+Show Affiliations

Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas, USP, Ribeirão Preto, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

18551599

Citation

Dos Santos, Harryson Wings Godoy, et al. "A Simple One-step Real-time RT-PCR for Diagnosis of Dengue Virus Infection." Journal of Medical Virology, vol. 80, no. 8, 2008, pp. 1426-33.
Dos Santos HW, Poloni TR, Souza KP, et al. A simple one-step real-time RT-PCR for diagnosis of dengue virus infection. J Med Virol. 2008;80(8):1426-33.
Dos Santos, H. W., Poloni, T. R., Souza, K. P., Muller, V. D., Tremeschin, F., Nali, L. C., Fantinatti, L. R., Amarilla, A. A., Castro, H. L., Nunes, M. R., Casseb, S. M., Vasconcelos, P. F., Badra, S. J., Figueiredo, L. T., & Aquino, V. H. (2008). A simple one-step real-time RT-PCR for diagnosis of dengue virus infection. Journal of Medical Virology, 80(8), 1426-33. https://doi.org/10.1002/jmv.21203
Dos Santos HW, et al. A Simple One-step Real-time RT-PCR for Diagnosis of Dengue Virus Infection. J Med Virol. 2008;80(8):1426-33. PubMed PMID: 18551599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A simple one-step real-time RT-PCR for diagnosis of dengue virus infection. AU - Dos Santos,Harryson Wings Godoy, AU - Poloni,Telma Regina Ramos Silva, AU - Souza,Kelly Paula, AU - Muller,Vanessa Danielle Menjon, AU - Tremeschin,Flávia, AU - Nali,Lívia Christensen, AU - Fantinatti,Leandro Ricardo, AU - Amarilla,Alberto Anastacio, AU - Castro,Helda Liz Alfonso, AU - Nunes,Marcio Roberto, AU - Casseb,Samir Mansour, AU - Vasconcelos,Pedro Fernando, AU - Badra,Soraya Jabur, AU - Figueiredo,Luiz Tadeu Moraes, AU - Aquino,Victor Hugo, PY - 2008/6/14/pubmed PY - 2008/8/30/medline PY - 2008/6/14/entrez SP - 1426 EP - 33 JF - Journal of medical virology JO - J Med Virol VL - 80 IS - 8 N2 - Dengue is the most important arbovirus disease in tropical and sub-tropical countries, and can be caused by infection with any of the four-dengue virus (DENV) serotypes. Infection with DENV can lead to a broad clinical spectrum, ranging from sub-clinical infection or an influenza-like disease known as dengue fever (DF) to a severe, sometimes fatal, disease characterized by hemorrhage and plasma leakage that can lead to shock, known as dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). The diagnosis of dengue is routinely accomplished by serologic assays, such as IgM and IgG ELISAs, as well as HI tests, analyzing serum samples obtained from patients with at least 7 days of symptoms onset. These tests cannot be used for diagnosis during the early symptomatic phase. In addition, antibodies against dengue are broad reactive with other flaviviruses. Therefore, a specific diagnostic method for acute DENV infection is of great interest. In that sense, the real-time RT-PCR has become an important tool that can be used for early and specific detection of dengue virus genome in human serum samples. This study describes a simple, specific, and sensitive real-time RT-PCR for early diagnosis of dengue virus infection. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/18551599/A_simple_one_step_real_time_RT_PCR_for_diagnosis_of_dengue_virus_infection_ L2 - https://doi.org/10.1002/jmv.21203 DB - PRIME DP - Unbound Medicine ER -