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Chiral analysis of amino acids from conventional and transgenic yeasts.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 01; 875(1):243-7.JC

Abstract

Autolysis of Saccharomyces cerevisiae yeast is the main source of molecules that contribute to the quality of sparkling wines made by the traditional method. In this work, a genetically modified yeast (LS11) is compared to its isogenic wild type strain (BY4741) after autolysis. Chiral micellar electrokinetic chromatography with laser-induced fluorescence detection (chiral-MEKC-LIF) is used to identify and quantify the main D- and L-amino acids from both strains after accelerated autolysis. The procedure includes amino acids extraction, derivatization with FITC and chiral-MEKC-LIF separation in a background electrolyte composed of 100 mM sodium tetraborate, 30 mM SDS, 20 mM beta-CD at pH 10.0. The D- and L-forms of Arg, Asn, Ala, Glu and Asp, corresponding to the major amino acids found in these samples, are separated in less than 30 min with efficiencies up to 800,000 plates/m and high sensitivity (i.e., LODs as low as 40 nM were obtained for D-Arg for a signal to noise ratio of three). From these results it is corroborated that the genetic modification brings a faster autolysis of the yeast, releasing a higher amount of L-amino acids to the medium in a short time. Interestingly, the pattern of release of D-amino acids was also different between the transgenic and the conventional yeast strains.

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Authors+Show Affiliations

Giuffrida A
Department of Chemical Sciences, University of Catania, Viale A. Doria 6, Catania, Italy.
Tabera L
No affiliation info available
González R
No affiliation info available
Cucinotta V
No affiliation info available
Cifuentes A
No affiliation info available

MeSH

Amino AcidsChromatography, Micellar Electrokinetic CapillaryOrganisms, Genetically ModifiedSaccharomyces cerevisiaeStereoisomerism

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18554993

Citation

Giuffrida, Alessandro, et al. "Chiral Analysis of Amino Acids From Conventional and Transgenic Yeasts." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 875, no. 1, 2008, pp. 243-7.
Giuffrida A, Tabera L, González R, et al. Chiral analysis of amino acids from conventional and transgenic yeasts. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;875(1):243-7.
Giuffrida, A., Tabera, L., González, R., Cucinotta, V., & Cifuentes, A. (2008). Chiral analysis of amino acids from conventional and transgenic yeasts. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 875(1), 243-7. https://doi.org/10.1016/j.jchromb.2008.05.039
Giuffrida A, et al. Chiral Analysis of Amino Acids From Conventional and Transgenic Yeasts. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 1;875(1):243-7. PubMed PMID: 18554993.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chiral analysis of amino acids from conventional and transgenic yeasts. AU - Giuffrida,Alessandro, AU - Tabera,Laura, AU - González,Ramón, AU - Cucinotta,Vincenzo, AU - Cifuentes,Alejandro, PY - 2008/03/07/received PY - 2008/05/10/revised PY - 2008/05/24/accepted PY - 2008/6/17/pubmed PY - 2009/3/25/medline PY - 2008/6/17/entrez SP - 243 EP - 7 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 875 IS - 1 N2 - Autolysis of Saccharomyces cerevisiae yeast is the main source of molecules that contribute to the quality of sparkling wines made by the traditional method. In this work, a genetically modified yeast (LS11) is compared to its isogenic wild type strain (BY4741) after autolysis. Chiral micellar electrokinetic chromatography with laser-induced fluorescence detection (chiral-MEKC-LIF) is used to identify and quantify the main D- and L-amino acids from both strains after accelerated autolysis. The procedure includes amino acids extraction, derivatization with FITC and chiral-MEKC-LIF separation in a background electrolyte composed of 100 mM sodium tetraborate, 30 mM SDS, 20 mM beta-CD at pH 10.0. The D- and L-forms of Arg, Asn, Ala, Glu and Asp, corresponding to the major amino acids found in these samples, are separated in less than 30 min with efficiencies up to 800,000 plates/m and high sensitivity (i.e., LODs as low as 40 nM were obtained for D-Arg for a signal to noise ratio of three). From these results it is corroborated that the genetic modification brings a faster autolysis of the yeast, releasing a higher amount of L-amino acids to the medium in a short time. Interestingly, the pattern of release of D-amino acids was also different between the transgenic and the conventional yeast strains. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18554993/Chiral_analysis_of_amino_acids_from_conventional_and_transgenic_yeasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00383-8 DB - PRIME DP - Unbound Medicine ER -