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The effects of interleukin-6 on the contraction and relaxation responses of the cavernous smooth muscle from rats.
Eur J Pharmacol. 2008 Jul 28; 589(1-3):228-32.EJ

Abstract

The purpose of this study is to elucidate the effect of IL-6 on the vasomotor reactivity of the corpus cavernosum of the rats. The strips were either left untreated or treated with 1 ng/ml of IL-6 for 60 min. By increasing concentrations of phenylephrine, acetylcholine, or sodium nitroprusside, we assessed concentration-contraction or relaxation responses. The IL-6-treated strips were incubated for 30 min with or without L-NAME (N(W)-nitro-L-arginine methyl ester), L-arginine, indomethacin, BQ-123 (an endothelin receptor A inhibitor), or SQ 29,548 (a thromboxane A(2) [TXA(2)] receptor blocker), and the effects on phenylephrine-induced contraction or acetylcholine-induced relaxation of phenylephrine-induced contraction were measured. The contractile responses to phenylephrine were significantly enhanced in the IL-6-treated strips, compared with the IL-6-nontreated strips, and the relaxation responses to acetylcholine were significantly inhibited in the IL-6-treated group compared with the IL-6-nontreated group. But after endothelial denudation, there was no difference between the IL-6-treated strips and the IL-6-nontreated strips on the contraction-relaxation responses to phenylephrine or acetylcholine. The relaxation responses to sodium nitroprusside were not inhibited in both groups. L-NAME completely inhibited the relaxation response to acetylcholine in the IL-6-treated strips, as well as the IL-6-nontreated strips. Indomethacin and SQ 29,548 significantly inhibited the increased contractile responses to phenylephrine in the IL-6-treated strips. But BQ 123 rarely affected the same responses. L-arginine reversed the inhibited relaxation responses to acetylcholine in the IL-6-treated strips. Therefore, IL-6 inhibits endothelium-dependent, NO-mediated relaxation and also enhances alpha(1)-adrenergic receptor-mediated contraction via an endothelium-dependent TXA(2)-mediated mechanism in the corpus cavernosum of the rat.

Authors+Show Affiliations

Department of Urology, Chung-Ang University Hospital, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18555215

Citation

Myung, Soon Chul, et al. "The Effects of Interleukin-6 On the Contraction and Relaxation Responses of the Cavernous Smooth Muscle From Rats." European Journal of Pharmacology, vol. 589, no. 1-3, 2008, pp. 228-32.
Myung SC, Han JH, Song KK, et al. The effects of interleukin-6 on the contraction and relaxation responses of the cavernous smooth muscle from rats. Eur J Pharmacol. 2008;589(1-3):228-32.
Myung, S. C., Han, J. H., Song, K. K., Kang, G. H., Lee, S. Y., Kim, T. H., Lee, M. Y., Kim, H. W., & Kim, S. C. (2008). The effects of interleukin-6 on the contraction and relaxation responses of the cavernous smooth muscle from rats. European Journal of Pharmacology, 589(1-3), 228-32. https://doi.org/10.1016/j.ejphar.2008.04.053
Myung SC, et al. The Effects of Interleukin-6 On the Contraction and Relaxation Responses of the Cavernous Smooth Muscle From Rats. Eur J Pharmacol. 2008 Jul 28;589(1-3):228-32. PubMed PMID: 18555215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of interleukin-6 on the contraction and relaxation responses of the cavernous smooth muscle from rats. AU - Myung,Soon Chul, AU - Han,June Hyun, AU - Song,Kee Keun, AU - Kang,Gun Hyun, AU - Lee,Shin Young, AU - Kim,Tae Hyoung, AU - Lee,Moo Yeol, AU - Kim,Hyun Woo, AU - Kim,Sae-Chul, Y1 - 2008/05/05/ PY - 2007/10/19/received PY - 2008/03/05/revised PY - 2008/04/24/accepted PY - 2008/6/17/pubmed PY - 2008/10/25/medline PY - 2008/6/17/entrez SP - 228 EP - 32 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 589 IS - 1-3 N2 - The purpose of this study is to elucidate the effect of IL-6 on the vasomotor reactivity of the corpus cavernosum of the rats. The strips were either left untreated or treated with 1 ng/ml of IL-6 for 60 min. By increasing concentrations of phenylephrine, acetylcholine, or sodium nitroprusside, we assessed concentration-contraction or relaxation responses. The IL-6-treated strips were incubated for 30 min with or without L-NAME (N(W)-nitro-L-arginine methyl ester), L-arginine, indomethacin, BQ-123 (an endothelin receptor A inhibitor), or SQ 29,548 (a thromboxane A(2) [TXA(2)] receptor blocker), and the effects on phenylephrine-induced contraction or acetylcholine-induced relaxation of phenylephrine-induced contraction were measured. The contractile responses to phenylephrine were significantly enhanced in the IL-6-treated strips, compared with the IL-6-nontreated strips, and the relaxation responses to acetylcholine were significantly inhibited in the IL-6-treated group compared with the IL-6-nontreated group. But after endothelial denudation, there was no difference between the IL-6-treated strips and the IL-6-nontreated strips on the contraction-relaxation responses to phenylephrine or acetylcholine. The relaxation responses to sodium nitroprusside were not inhibited in both groups. L-NAME completely inhibited the relaxation response to acetylcholine in the IL-6-treated strips, as well as the IL-6-nontreated strips. Indomethacin and SQ 29,548 significantly inhibited the increased contractile responses to phenylephrine in the IL-6-treated strips. But BQ 123 rarely affected the same responses. L-arginine reversed the inhibited relaxation responses to acetylcholine in the IL-6-treated strips. Therefore, IL-6 inhibits endothelium-dependent, NO-mediated relaxation and also enhances alpha(1)-adrenergic receptor-mediated contraction via an endothelium-dependent TXA(2)-mediated mechanism in the corpus cavernosum of the rat. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/18555215/The_effects_of_interleukin_6_on_the_contraction_and_relaxation_responses_of_the_cavernous_smooth_muscle_from_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(08)00482-2 DB - PRIME DP - Unbound Medicine ER -