Tags

Type your tag names separated by a space and hit enter

Stability of long term facilitation and expression of zif268 and Arc in the spinal cord dorsal horn is modulated by conditioning stimulation within the physiological frequency range of primary afferent fibers.
Neuroscience. 2008 Jul 17; 154(4):1568-75.N

Abstract

Long term facilitation (LTF) of C-fiber-evoked firing of wide dynamic range neurons in the spinal dorsal horn in response to conditioning stimulation (CS) of afferent fibers is a widely studied cellular model of spinal nociceptive sensitization. Although 100 Hz CS of primary afferent fibers is commonly used to induce spinal cord LTF, this frequency exceeds the physiological firing range. Here, we examined the effects of electrical stimulation of the sciatic nerve within the physiological frequency range on the magnitude and stability of the C-fiber-evoked responses of wide dynamic range neurons and the expression of immediate early genes (c-fos, zif268, and Arc) in anesthetized rats. Stimulation frequencies of 3, 30 and 100 Hz all induced facilitation of similar magnitude as recorded at 1 h post-CS. Strikingly, however, 3 Hz-induced potentiation of the C-fiber responses was decremental, whereas both 30 and 100 Hz stimulation resulted in stable, non-decremental facilitation over 3 h of recording. The number of dorsal horn neurons expressing c-fos, but not zif268 or Arc, was significantly elevated after 3 Hz CS and increased proportionally with stimulation rate. In contrast, a stable LTF of C-fiber responses was obtained at 30 and 100 Hz CS, and at these frequencies there was a sharp increase in zif268 expression and appearance of Arc-positive neurons. The results show that response facilitation can be induced by stimulation frequencies in the physiological range (3 and 30 Hz). Three hertz stimulation induced the early phase of LTF, but the responses were decremental. Arc and zif268, two genes previously coupled to LTP of synaptic transmission in the adult brain, are upregulated at the same frequencies that give stable LTF (30 and 100 Hz). This frequency-dependence is important for understanding how the afferent firing pattern affects neuronal plasticity and nociception in the spinal dorsal horn.

Authors+Show Affiliations

Department of Biomedicine, University of Bergen, Bergen, Norway.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18555615

Citation

Haugan, F, et al. "Stability of Long Term Facilitation and Expression of Zif268 and Arc in the Spinal Cord Dorsal Horn Is Modulated By Conditioning Stimulation Within the Physiological Frequency Range of Primary Afferent Fibers." Neuroscience, vol. 154, no. 4, 2008, pp. 1568-75.
Haugan F, Wibrand K, Fiskå A, et al. Stability of long term facilitation and expression of zif268 and Arc in the spinal cord dorsal horn is modulated by conditioning stimulation within the physiological frequency range of primary afferent fibers. Neuroscience. 2008;154(4):1568-75.
Haugan, F., Wibrand, K., Fiskå, A., Bramham, C. R., & Tjølsen, A. (2008). Stability of long term facilitation and expression of zif268 and Arc in the spinal cord dorsal horn is modulated by conditioning stimulation within the physiological frequency range of primary afferent fibers. Neuroscience, 154(4), 1568-75. https://doi.org/10.1016/j.neuroscience.2008.05.010
Haugan F, et al. Stability of Long Term Facilitation and Expression of Zif268 and Arc in the Spinal Cord Dorsal Horn Is Modulated By Conditioning Stimulation Within the Physiological Frequency Range of Primary Afferent Fibers. Neuroscience. 2008 Jul 17;154(4):1568-75. PubMed PMID: 18555615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stability of long term facilitation and expression of zif268 and Arc in the spinal cord dorsal horn is modulated by conditioning stimulation within the physiological frequency range of primary afferent fibers. AU - Haugan,F, AU - Wibrand,K, AU - Fiskå,A, AU - Bramham,C R, AU - Tjølsen,A, Y1 - 2008/05/21/ PY - 2008/03/04/received PY - 2008/05/12/revised PY - 2008/05/12/accepted PY - 2008/6/17/pubmed PY - 2009/6/30/medline PY - 2008/6/17/entrez SP - 1568 EP - 75 JF - Neuroscience JO - Neuroscience VL - 154 IS - 4 N2 - Long term facilitation (LTF) of C-fiber-evoked firing of wide dynamic range neurons in the spinal dorsal horn in response to conditioning stimulation (CS) of afferent fibers is a widely studied cellular model of spinal nociceptive sensitization. Although 100 Hz CS of primary afferent fibers is commonly used to induce spinal cord LTF, this frequency exceeds the physiological firing range. Here, we examined the effects of electrical stimulation of the sciatic nerve within the physiological frequency range on the magnitude and stability of the C-fiber-evoked responses of wide dynamic range neurons and the expression of immediate early genes (c-fos, zif268, and Arc) in anesthetized rats. Stimulation frequencies of 3, 30 and 100 Hz all induced facilitation of similar magnitude as recorded at 1 h post-CS. Strikingly, however, 3 Hz-induced potentiation of the C-fiber responses was decremental, whereas both 30 and 100 Hz stimulation resulted in stable, non-decremental facilitation over 3 h of recording. The number of dorsal horn neurons expressing c-fos, but not zif268 or Arc, was significantly elevated after 3 Hz CS and increased proportionally with stimulation rate. In contrast, a stable LTF of C-fiber responses was obtained at 30 and 100 Hz CS, and at these frequencies there was a sharp increase in zif268 expression and appearance of Arc-positive neurons. The results show that response facilitation can be induced by stimulation frequencies in the physiological range (3 and 30 Hz). Three hertz stimulation induced the early phase of LTF, but the responses were decremental. Arc and zif268, two genes previously coupled to LTP of synaptic transmission in the adult brain, are upregulated at the same frequencies that give stable LTF (30 and 100 Hz). This frequency-dependence is important for understanding how the afferent firing pattern affects neuronal plasticity and nociception in the spinal dorsal horn. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/18555615/Stability_of_long_term_facilitation_and_expression_of_zif268_and_Arc_in_the_spinal_cord_dorsal_horn_is_modulated_by_conditioning_stimulation_within_the_physiological_frequency_range_of_primary_afferent_fibers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(08)00757-4 DB - PRIME DP - Unbound Medicine ER -