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The hyperenergetic-fed obese dog, a model of disturbance of apolipoprotein B-100 metabolism associated with insulin resistance: kinetic study using stable isotopes.
Metabolism. 2008 Jul; 57(7):966-72.M

Abstract

The hyperenergetic-fed beagle dog model of obesity-associated insulin resistance has previously demonstrated lipoprotein abnormalities similar to those of obese insulin-resistant humans. The aim of this study was to check, in the insulin-resistant dog, the mechanism leading to abnormalities in the mass of apolipoprotein B-100 (apo B-100) containing lipoproteins. Six healthy male beagle dogs were overfed with a high-fat diet for 28 +/- 2.5 weeks. Obesity was associated with insulin resistance as assessed by the euglycemic hyperinsulinemic clamp technique. The kinetics of very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) apo B-100 were recorded in dogs, at healthy and insulin-resistant states, using a primed constant infusion of [5,5,5-D(3)]leucine. Isotopic enrichment was measured by gas chromatography-mass spectrometry (GC-MS). A multicompartmental model was used for the analysis of tracer kinetics data. Apolipoprotein B-100 concentration was higher in VLDL (2.8-fold, P < .05) but lower in LDL (2-fold, P < .05) in the insulin-resistant compared to the healthy state. Kinetic analysis showed a higher VLDL apo B-100 production (1.7-fold, P < .05). The fractional catabolic rate of VLDL did not change significantly, but the lipolysis was decreased significantly (3-fold, P < .05). The lower LDL apo B-100 level in insulin-resistant dogs was explained by a higher LDL fractional catabolic rate (2.5-fold, P < .05). The mechanisms leading to hypertriglyceridemia (higher production rate and lower lipolysis of VLDL) in insulin-resistant dogs were similar to those described in the insulin-resistant humans.

Authors+Show Affiliations

Centre de Recherche en Nutrition Humaine, Institut national de la santé et de la recherche médicale, INSERM U539, CHU Nantes F-44000, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18555839

Citation

Briand, François, et al. "The Hyperenergetic-fed Obese Dog, a Model of Disturbance of Apolipoprotein B-100 Metabolism Associated With Insulin Resistance: Kinetic Study Using Stable Isotopes." Metabolism: Clinical and Experimental, vol. 57, no. 7, 2008, pp. 966-72.
Briand F, Bailhache E, Andre A, et al. The hyperenergetic-fed obese dog, a model of disturbance of apolipoprotein B-100 metabolism associated with insulin resistance: kinetic study using stable isotopes. Metabolism. 2008;57(7):966-72.
Briand, F., Bailhache, E., Andre, A., Magot, T., Krempf, M., Nguyen, P., & Ouguerram, K. (2008). The hyperenergetic-fed obese dog, a model of disturbance of apolipoprotein B-100 metabolism associated with insulin resistance: kinetic study using stable isotopes. Metabolism: Clinical and Experimental, 57(7), 966-72. https://doi.org/10.1016/j.metabol.2008.02.013
Briand F, et al. The Hyperenergetic-fed Obese Dog, a Model of Disturbance of Apolipoprotein B-100 Metabolism Associated With Insulin Resistance: Kinetic Study Using Stable Isotopes. Metabolism. 2008;57(7):966-72. PubMed PMID: 18555839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The hyperenergetic-fed obese dog, a model of disturbance of apolipoprotein B-100 metabolism associated with insulin resistance: kinetic study using stable isotopes. AU - Briand,François, AU - Bailhache,Edwige, AU - Andre,Agnes, AU - Magot,Thierry, AU - Krempf,Michel, AU - Nguyen,Patrick, AU - Ouguerram,Khadija, PY - 2007/09/17/received PY - 2008/02/15/accepted PY - 2008/6/17/pubmed PY - 2008/7/23/medline PY - 2008/6/17/entrez SP - 966 EP - 72 JF - Metabolism: clinical and experimental JO - Metabolism VL - 57 IS - 7 N2 - The hyperenergetic-fed beagle dog model of obesity-associated insulin resistance has previously demonstrated lipoprotein abnormalities similar to those of obese insulin-resistant humans. The aim of this study was to check, in the insulin-resistant dog, the mechanism leading to abnormalities in the mass of apolipoprotein B-100 (apo B-100) containing lipoproteins. Six healthy male beagle dogs were overfed with a high-fat diet for 28 +/- 2.5 weeks. Obesity was associated with insulin resistance as assessed by the euglycemic hyperinsulinemic clamp technique. The kinetics of very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) apo B-100 were recorded in dogs, at healthy and insulin-resistant states, using a primed constant infusion of [5,5,5-D(3)]leucine. Isotopic enrichment was measured by gas chromatography-mass spectrometry (GC-MS). A multicompartmental model was used for the analysis of tracer kinetics data. Apolipoprotein B-100 concentration was higher in VLDL (2.8-fold, P < .05) but lower in LDL (2-fold, P < .05) in the insulin-resistant compared to the healthy state. Kinetic analysis showed a higher VLDL apo B-100 production (1.7-fold, P < .05). The fractional catabolic rate of VLDL did not change significantly, but the lipolysis was decreased significantly (3-fold, P < .05). The lower LDL apo B-100 level in insulin-resistant dogs was explained by a higher LDL fractional catabolic rate (2.5-fold, P < .05). The mechanisms leading to hypertriglyceridemia (higher production rate and lower lipolysis of VLDL) in insulin-resistant dogs were similar to those described in the insulin-resistant humans. SN - 0026-0495 UR - https://www.unboundmedicine.com/medline/citation/18555839/The_hyperenergetic_fed_obese_dog_a_model_of_disturbance_of_apolipoprotein_B_100_metabolism_associated_with_insulin_resistance:_kinetic_study_using_stable_isotopes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0026-0495(08)00084-X DB - PRIME DP - Unbound Medicine ER -