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Biotin-containing phospholipid vesicle layer formed on self-assembled monolayer of a saccharide-terminated alkyl disulfide for surface plasmon resonance biosensing.
J Biosci Bioeng. 2008 May; 105(5):527-35.JB

Abstract

We describe a technique to form a biotin-containing phospholipid vesicle layer on a self-assembled monolayer (SAM) deposited on a gold surface to immobilize biotinylated receptor proteins for a surface plasmon resonance (SPR) biosensor. The adsorption of vesicle of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) was examined by SPR on the SAMs of dithiobis(1-deoxy-glucitol-1-carbamoyl pentane) (DDGP), 11-mercaptoundecanoic acid, 11-mercaptoundecanol, 11-amino-1-undecanethiol, and 12-mercaptododecane, and it was found that the DOPC vesicle rapidly adsorbed on the DDGP SAM to achieve the highest coverage of the surface. By quartz crystal microbalance with dissipation monitoring (QCM-D), the DOPC layer formed on the DDGP SAM was shown to be a vesicle layer, in which intact DOPC vesicles physisorbed on the SAM surface. To immobilize a biotinylated receptor protein, one of three biotinylated phospholipids, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(biotinyl) (biotin-DOPE), N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X-DHPE) and N-(biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-DHPE), was mixed with DOPC to form a biotin-containing vesicle layer on the DDGP SAM. A comparative binding study of NeutrAvidin and the biotin-containing vesicle layers showed that the use of biotin-X-DHPE achieved the most rapid immobilization of NeutrAvidin on the vesicle layer at the highest surface density. Furthermore, biotinylated protein A, as a receptor protein, could be immobilized through NeutrAvidin on the vesicle layer containing DOPC and biotin-X-DHPE, and its reaction with immunoglobulin G, as an analyte, was successfully observed by SPR. The results demonstrate that the biotin-containing vesicle layer on the DDGP SAM must be a useful component for SPR biosensor surfaces.

Authors+Show Affiliations

OMRON-Endowed Chair in Nano Optical Devices, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18558345

Citation

Ishizuka-Katsura, Yoshiko, et al. "Biotin-containing Phospholipid Vesicle Layer Formed On Self-assembled Monolayer of a Saccharide-terminated Alkyl Disulfide for Surface Plasmon Resonance Biosensing." Journal of Bioscience and Bioengineering, vol. 105, no. 5, 2008, pp. 527-35.
Ishizuka-Katsura Y, Wazawa T, Ban T, et al. Biotin-containing phospholipid vesicle layer formed on self-assembled monolayer of a saccharide-terminated alkyl disulfide for surface plasmon resonance biosensing. J Biosci Bioeng. 2008;105(5):527-35.
Ishizuka-Katsura, Y., Wazawa, T., Ban, T., Morigaki, K., & Aoyama, S. (2008). Biotin-containing phospholipid vesicle layer formed on self-assembled monolayer of a saccharide-terminated alkyl disulfide for surface plasmon resonance biosensing. Journal of Bioscience and Bioengineering, 105(5), 527-35. https://doi.org/10.1263/jbb.105.527
Ishizuka-Katsura Y, et al. Biotin-containing Phospholipid Vesicle Layer Formed On Self-assembled Monolayer of a Saccharide-terminated Alkyl Disulfide for Surface Plasmon Resonance Biosensing. J Biosci Bioeng. 2008;105(5):527-35. PubMed PMID: 18558345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biotin-containing phospholipid vesicle layer formed on self-assembled monolayer of a saccharide-terminated alkyl disulfide for surface plasmon resonance biosensing. AU - Ishizuka-Katsura,Yoshiko, AU - Wazawa,Tetsuichi, AU - Ban,Tadato, AU - Morigaki,Kenichi, AU - Aoyama,Shigeru, PY - 2007/08/29/received PY - 2008/02/19/accepted PY - 2008/6/19/pubmed PY - 2008/9/11/medline PY - 2008/6/19/entrez SP - 527 EP - 35 JF - Journal of bioscience and bioengineering JO - J Biosci Bioeng VL - 105 IS - 5 N2 - We describe a technique to form a biotin-containing phospholipid vesicle layer on a self-assembled monolayer (SAM) deposited on a gold surface to immobilize biotinylated receptor proteins for a surface plasmon resonance (SPR) biosensor. The adsorption of vesicle of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) was examined by SPR on the SAMs of dithiobis(1-deoxy-glucitol-1-carbamoyl pentane) (DDGP), 11-mercaptoundecanoic acid, 11-mercaptoundecanol, 11-amino-1-undecanethiol, and 12-mercaptododecane, and it was found that the DOPC vesicle rapidly adsorbed on the DDGP SAM to achieve the highest coverage of the surface. By quartz crystal microbalance with dissipation monitoring (QCM-D), the DOPC layer formed on the DDGP SAM was shown to be a vesicle layer, in which intact DOPC vesicles physisorbed on the SAM surface. To immobilize a biotinylated receptor protein, one of three biotinylated phospholipids, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(biotinyl) (biotin-DOPE), N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X-DHPE) and N-(biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-DHPE), was mixed with DOPC to form a biotin-containing vesicle layer on the DDGP SAM. A comparative binding study of NeutrAvidin and the biotin-containing vesicle layers showed that the use of biotin-X-DHPE achieved the most rapid immobilization of NeutrAvidin on the vesicle layer at the highest surface density. Furthermore, biotinylated protein A, as a receptor protein, could be immobilized through NeutrAvidin on the vesicle layer containing DOPC and biotin-X-DHPE, and its reaction with immunoglobulin G, as an analyte, was successfully observed by SPR. The results demonstrate that the biotin-containing vesicle layer on the DDGP SAM must be a useful component for SPR biosensor surfaces. SN - 1347-4421 UR - https://www.unboundmedicine.com/medline/citation/18558345/Biotin_containing_phospholipid_vesicle_layer_formed_on_self_assembled_monolayer_of_a_saccharide_terminated_alkyl_disulfide_for_surface_plasmon_resonance_biosensing_ DB - PRIME DP - Unbound Medicine ER -