Tags

Type your tag names separated by a space and hit enter

[Influence of Feixian Formula on serum transforming growth factor-beta1 and platelet-derived growth factor in rats with bleomycin-induced pulmonary fibrosis].
Zhong Xi Yi Jie He Xue Bao. 2008 Jun; 6(6):595-9.ZX

Abstract

OBJECTIVE

To observe the effects of Feixian Formula, a compound traditional Chinese herbal medicine for treating pulmonary fibrosis, on bleomycin-induced pulmonary fibrosis in rats, and its influence on serum transforming growth factor-beta1 (TGF-beta1) and platelet-derived growth factor (PDGF).

METHODS

Seventy-two male Wistar rats were infused with bleomycin (1 mg/kg) through tracheal intubation to induce pulmonary fibrosis, and they were randomly divided into untreated group (n=24), prednisone-treated group (n=24) and Feixian Formula-treated group (n=24). Fifteen male Wistar rats of the sham-operated group were infused with equivalent normal saline. Twenty-four hours after operation, prednisone (5 mg/kg) and Feixian Formula (1.25 g/kg) were given to the prednisone-treated group and Feixian Formula-treated group respectively by intragastric administration once a day. Equivalent saline was administered to rats of the untreated group and sham-operated group. On the 14th, 28th and 45th day, 5 rats in the sham-operated group and 8 rats in each of the other three groups were dissected to observe pathologic changes of the lung tissues, and the levels of serum TGF-beta1 and PDGF were determined by enzyme-linked immunosorbent assay.

RESULTS

At the 45th day, the degree of pulmonary interstitial fibrosis was lesser in rats of the Feixian Formula-treated group as compared with those of the untreated group and prednisone-treated group. The levels of serum TGF-beta1 and PDGF were increased, and were significantly higher than those of the sham-operated group, especially on the 45th day (P<0.05). Changes of TGF-beta1 level in the prednisone-treated group and the Feixian Formula-treated group were similar to untreated group (P>0.05), and there was no significant difference between the prednisone-treated group and the Feixian Formula-treated group (P>0.05). PDGF in the Feixian Formula-treated group reached the highest level on the 14th day, significantly higher than those of the other three groups (P<0.01). Then it decreased, and was close to that of the sham-operated group on the 45th day (P=0.792). The levels of PDGF in untreated group and prednisone-treated group were increased depending on time, and were obviously higher than that of the sham-operated group on the 45th day (P<0.01).

CONCLUSION

Feixian Formula can relieve bleomycin-induced pulmonary fibrosis in rats, and the mechanism of its action may be related to down-regulating serum PDGF.

Authors+Show Affiliations

Department of Respiratory Diseases, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18559237

Citation

Zhang, Wei, et al. "[Influence of Feixian Formula On Serum Transforming Growth Factor-beta1 and Platelet-derived Growth Factor in Rats With Bleomycin-induced Pulmonary Fibrosis]." Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine, vol. 6, no. 6, 2008, pp. 595-9.
Zhang W, Jiang LD, Zhang XM, et al. [Influence of Feixian Formula on serum transforming growth factor-beta1 and platelet-derived growth factor in rats with bleomycin-induced pulmonary fibrosis]. Zhong Xi Yi Jie He Xue Bao. 2008;6(6):595-9.
Zhang, W., Jiang, L. D., Zhang, X. M., Wu, J. J., Deng, Y., & Lu, X. F. (2008). [Influence of Feixian Formula on serum transforming growth factor-beta1 and platelet-derived growth factor in rats with bleomycin-induced pulmonary fibrosis]. Zhong Xi Yi Jie He Xue Bao = Journal of Chinese Integrative Medicine, 6(6), 595-9. https://doi.org/10.3736/jcim20080610
Zhang W, et al. [Influence of Feixian Formula On Serum Transforming Growth Factor-beta1 and Platelet-derived Growth Factor in Rats With Bleomycin-induced Pulmonary Fibrosis]. Zhong Xi Yi Jie He Xue Bao. 2008;6(6):595-9. PubMed PMID: 18559237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Influence of Feixian Formula on serum transforming growth factor-beta1 and platelet-derived growth factor in rats with bleomycin-induced pulmonary fibrosis]. AU - Zhang,Wei, AU - Jiang,Liang-duo, AU - Zhang,Xiao-mei, AU - Wu,Jian-jun, AU - Deng,Yuan, AU - Lu,Xiang-feng, PY - 2008/6/19/pubmed PY - 2010/6/25/medline PY - 2008/6/19/entrez SP - 595 EP - 9 JF - Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine JO - Zhong Xi Yi Jie He Xue Bao VL - 6 IS - 6 N2 - OBJECTIVE: To observe the effects of Feixian Formula, a compound traditional Chinese herbal medicine for treating pulmonary fibrosis, on bleomycin-induced pulmonary fibrosis in rats, and its influence on serum transforming growth factor-beta1 (TGF-beta1) and platelet-derived growth factor (PDGF). METHODS: Seventy-two male Wistar rats were infused with bleomycin (1 mg/kg) through tracheal intubation to induce pulmonary fibrosis, and they were randomly divided into untreated group (n=24), prednisone-treated group (n=24) and Feixian Formula-treated group (n=24). Fifteen male Wistar rats of the sham-operated group were infused with equivalent normal saline. Twenty-four hours after operation, prednisone (5 mg/kg) and Feixian Formula (1.25 g/kg) were given to the prednisone-treated group and Feixian Formula-treated group respectively by intragastric administration once a day. Equivalent saline was administered to rats of the untreated group and sham-operated group. On the 14th, 28th and 45th day, 5 rats in the sham-operated group and 8 rats in each of the other three groups were dissected to observe pathologic changes of the lung tissues, and the levels of serum TGF-beta1 and PDGF were determined by enzyme-linked immunosorbent assay. RESULTS: At the 45th day, the degree of pulmonary interstitial fibrosis was lesser in rats of the Feixian Formula-treated group as compared with those of the untreated group and prednisone-treated group. The levels of serum TGF-beta1 and PDGF were increased, and were significantly higher than those of the sham-operated group, especially on the 45th day (P<0.05). Changes of TGF-beta1 level in the prednisone-treated group and the Feixian Formula-treated group were similar to untreated group (P>0.05), and there was no significant difference between the prednisone-treated group and the Feixian Formula-treated group (P>0.05). PDGF in the Feixian Formula-treated group reached the highest level on the 14th day, significantly higher than those of the other three groups (P<0.01). Then it decreased, and was close to that of the sham-operated group on the 45th day (P=0.792). The levels of PDGF in untreated group and prednisone-treated group were increased depending on time, and were obviously higher than that of the sham-operated group on the 45th day (P<0.01). CONCLUSION: Feixian Formula can relieve bleomycin-induced pulmonary fibrosis in rats, and the mechanism of its action may be related to down-regulating serum PDGF. SN - 1672-1977 UR - https://www.unboundmedicine.com/medline/citation/18559237/[Influence_of_Feixian_Formula_on_serum_transforming_growth_factor_beta1_and_platelet_derived_growth_factor_in_rats_with_bleomycin_induced_pulmonary_fibrosis]_ L2 - https://medlineplus.gov/herbalmedicine.html DB - PRIME DP - Unbound Medicine ER -