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Pathogen-related spirochetes identified within gingival tissue from patients with acute necrotizing ulcerative gingivitis.
Infect Immun. 1991 Aug; 59(8):2653-7.II

Abstract

The purpose of this investigation was to determine whether monoclonal antibodies against pathogen-restricted antigens of Treponema pallidum subsp. pallidum could be used as probes for spirochetes in diseased gingival tissue from subjects with acute necrotizing ulcerative gingivitis. A biotin-streptavidin system was used to identify spirochetes bound by monoclonal antibodies in cryostat sections of tissue. Twelve of 16 tissue samples from diseased sites, but none of 8 tissue specimens from healthy sites, reacted with pathogen-restricted antibodies. Organisms were found in intact epithelium and connective tissues adjacent to ulcers. Staining intensity was often high in perivascular locations and around vesicular spaces. Monoclonal antibodies to Bacteroides gingivalis and Treponema denticola were each reactive with diseased gingival tissues, but staining was usually restricted to ulcerated areas. These studies extend recent observations that showed that subjects with acute necrotizing ulcerative gingivitis had both pathogen-related spirochetes in dental plaque and serum immunoglobulin G to pathogen-restricted antigens on T. pallidum subspecies, suggesting that pathogen-related spirochetes may be associated with the pathogenesis of certain periodontal diseases.

Authors+Show Affiliations

Department of Pediatric Dentistry, School of Dentistry, Oregon Health Sciences University, Portland 97201-3097.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1855985

Citation

Riviere, G R., et al. "Pathogen-related Spirochetes Identified Within Gingival Tissue From Patients With Acute Necrotizing Ulcerative Gingivitis." Infection and Immunity, vol. 59, no. 8, 1991, pp. 2653-7.
Riviere GR, Weisz KS, Simonson LG, et al. Pathogen-related spirochetes identified within gingival tissue from patients with acute necrotizing ulcerative gingivitis. Infect Immun. 1991;59(8):2653-7.
Riviere, G. R., Weisz, K. S., Simonson, L. G., & Lukehart, S. A. (1991). Pathogen-related spirochetes identified within gingival tissue from patients with acute necrotizing ulcerative gingivitis. Infection and Immunity, 59(8), 2653-7.
Riviere GR, et al. Pathogen-related Spirochetes Identified Within Gingival Tissue From Patients With Acute Necrotizing Ulcerative Gingivitis. Infect Immun. 1991;59(8):2653-7. PubMed PMID: 1855985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pathogen-related spirochetes identified within gingival tissue from patients with acute necrotizing ulcerative gingivitis. AU - Riviere,G R, AU - Weisz,K S, AU - Simonson,L G, AU - Lukehart,S A, PY - 1991/8/1/pubmed PY - 1991/8/1/medline PY - 1991/8/1/entrez SP - 2653 EP - 7 JF - Infection and immunity JO - Infect. Immun. VL - 59 IS - 8 N2 - The purpose of this investigation was to determine whether monoclonal antibodies against pathogen-restricted antigens of Treponema pallidum subsp. pallidum could be used as probes for spirochetes in diseased gingival tissue from subjects with acute necrotizing ulcerative gingivitis. A biotin-streptavidin system was used to identify spirochetes bound by monoclonal antibodies in cryostat sections of tissue. Twelve of 16 tissue samples from diseased sites, but none of 8 tissue specimens from healthy sites, reacted with pathogen-restricted antibodies. Organisms were found in intact epithelium and connective tissues adjacent to ulcers. Staining intensity was often high in perivascular locations and around vesicular spaces. Monoclonal antibodies to Bacteroides gingivalis and Treponema denticola were each reactive with diseased gingival tissues, but staining was usually restricted to ulcerated areas. These studies extend recent observations that showed that subjects with acute necrotizing ulcerative gingivitis had both pathogen-related spirochetes in dental plaque and serum immunoglobulin G to pathogen-restricted antigens on T. pallidum subspecies, suggesting that pathogen-related spirochetes may be associated with the pathogenesis of certain periodontal diseases. SN - 0019-9567 UR - https://www.unboundmedicine.com/medline/citation/1855985/Pathogen_related_spirochetes_identified_within_gingival_tissue_from_patients_with_acute_necrotizing_ulcerative_gingivitis_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=1855985 DB - PRIME DP - Unbound Medicine ER -