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Uncoupling and oxidative stress in liver mitochondria isolated from rats with acute iron overload.
Arch Toxicol. 2009 Jan; 83(1):47-53.AT

Abstract

One hypothesis for the etiology of cell damage arising from iron overload is that its excess selectively affects mitochondria. Here we tested the effects of acute iron overload on liver mitochondria isolated from rats subjected to a single dose of i.p. 500 mg/kg iron-dextran. The treatment increased the levels of iron in mitochondria (from 21 +/- 4 to 130 +/- 7 nmol/mg protein) and caused both lipid peroxidation and glutathione oxidation. The mitochondria of iron-treated rats showed lower respiratory control ratio in association with higher resting respiration. The mitochondrial uncoupling elicited by iron-treatment did not affect the phosphorylation efficiency or the ATP levels, suggesting that uncoupling is a mitochondrial protective mechanism against acute iron overload. Therefore, the reactive oxygen species (ROS)/H+ leak couple, functioning as a mitochondrial redox homeostatic mechanism could play a protective role in the acutely iron-loaded mitochondria.

Authors+Show Affiliations

Departamento de Investigaciones Biomédicas, Centro de Química Farmacéutica, Calle 200, Esq. 21, Playa, CP 11600, Ciudad de La Habana, Cuba. gilbertopardo@infomed.sld.cuNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18560806

Citation

Pardo Andreu, G L., et al. "Uncoupling and Oxidative Stress in Liver Mitochondria Isolated From Rats With Acute Iron Overload." Archives of Toxicology, vol. 83, no. 1, 2009, pp. 47-53.
Pardo Andreu GL, Inada NM, Vercesi AE, et al. Uncoupling and oxidative stress in liver mitochondria isolated from rats with acute iron overload. Arch Toxicol. 2009;83(1):47-53.
Pardo Andreu, G. L., Inada, N. M., Vercesi, A. E., & Curti, C. (2009). Uncoupling and oxidative stress in liver mitochondria isolated from rats with acute iron overload. Archives of Toxicology, 83(1), 47-53. https://doi.org/10.1007/s00204-008-0322-x
Pardo Andreu GL, et al. Uncoupling and Oxidative Stress in Liver Mitochondria Isolated From Rats With Acute Iron Overload. Arch Toxicol. 2009;83(1):47-53. PubMed PMID: 18560806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Uncoupling and oxidative stress in liver mitochondria isolated from rats with acute iron overload. AU - Pardo Andreu,G L, AU - Inada,N M, AU - Vercesi,A E, AU - Curti,C, Y1 - 2008/06/17/ PY - 2008/02/04/received PY - 2008/05/21/accepted PY - 2008/6/19/pubmed PY - 2009/3/5/medline PY - 2008/6/19/entrez SP - 47 EP - 53 JF - Archives of toxicology JO - Arch Toxicol VL - 83 IS - 1 N2 - One hypothesis for the etiology of cell damage arising from iron overload is that its excess selectively affects mitochondria. Here we tested the effects of acute iron overload on liver mitochondria isolated from rats subjected to a single dose of i.p. 500 mg/kg iron-dextran. The treatment increased the levels of iron in mitochondria (from 21 +/- 4 to 130 +/- 7 nmol/mg protein) and caused both lipid peroxidation and glutathione oxidation. The mitochondria of iron-treated rats showed lower respiratory control ratio in association with higher resting respiration. The mitochondrial uncoupling elicited by iron-treatment did not affect the phosphorylation efficiency or the ATP levels, suggesting that uncoupling is a mitochondrial protective mechanism against acute iron overload. Therefore, the reactive oxygen species (ROS)/H+ leak couple, functioning as a mitochondrial redox homeostatic mechanism could play a protective role in the acutely iron-loaded mitochondria. SN - 1432-0738 UR - https://www.unboundmedicine.com/medline/citation/18560806/Uncoupling_and_oxidative_stress_in_liver_mitochondria_isolated_from_rats_with_acute_iron_overload_ L2 - https://doi.org/10.1007/s00204-008-0322-x DB - PRIME DP - Unbound Medicine ER -