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In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration.
Environ Toxicol. 2009 Apr; 24(2):200-9.ET

Abstract

Microcystin-LR (MC-LR) is a toxin produced by freshwater cyanobacteria and is a potential threat to human health. MC-LR has been shown to be both a specific inhibitor of serine/threonine protein phosphatases PP1 and PP2A and a potent tumor promoter in rat liver. However, the genotoxic potential of MCs remains unclear. In this article, we investigated the ability of MC-LR to induce DNA damage on rat hepatocytes following intravenous (iv) administration by using two in vivo genotoxicity assays: the unscheduled DNA synthesis (UDS) and the comet assays. The UDS assay measures DNA synthesis induced from the excision repair of DNA damaged regions and the comet assay is a very sensitive technique for detecting various forms of DNA damage. After an exposure time of 2-4 h or 12-16 h and a dose ranging from 12.5 to 50 microg/kg bw, no DNA damage could be observed in both assays on rat hepatocytes following iv administration. These findings have been discussed and compared with recently published genotoxic results obtained in other organs from mice after oral and intraperitoneal treatments to better understand the mechanism of action of this toxin in relation with its cancerogenicity potential.

Authors+Show Affiliations

AFSSA, Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches sur Médicaments Vétérinaires et Désinfectants, Unité de Toxicologie Génétique des Contaminants Alimentaire, La Haute Marche, 35302 Fougères Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18561296

Citation

Gaudin, Julien, et al. "In Vivo Genotoxic Potential of microcystin-LR: a Cyanobacterial Toxin, Investigated Both By the Unscheduled DNA Synthesis (UDS) and the Comet Assays After Intravenous Administration." Environmental Toxicology, vol. 24, no. 2, 2009, pp. 200-9.
Gaudin J, Le Hegarat L, Nesslany F, et al. In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration. Environ Toxicol. 2009;24(2):200-9.
Gaudin, J., Le Hegarat, L., Nesslany, F., Marzin, D., & Fessard, V. (2009). In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration. Environmental Toxicology, 24(2), 200-9. https://doi.org/10.1002/tox.20417
Gaudin J, et al. In Vivo Genotoxic Potential of microcystin-LR: a Cyanobacterial Toxin, Investigated Both By the Unscheduled DNA Synthesis (UDS) and the Comet Assays After Intravenous Administration. Environ Toxicol. 2009;24(2):200-9. PubMed PMID: 18561296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo genotoxic potential of microcystin-LR: a cyanobacterial toxin, investigated both by the unscheduled DNA synthesis (UDS) and the comet assays after intravenous administration. AU - Gaudin,Julien, AU - Le Hegarat,Ludovic, AU - Nesslany,Fabrice, AU - Marzin,Daniel, AU - Fessard,Valérie, PY - 2008/6/19/pubmed PY - 2009/5/27/medline PY - 2008/6/19/entrez SP - 200 EP - 9 JF - Environmental toxicology JO - Environ Toxicol VL - 24 IS - 2 N2 - Microcystin-LR (MC-LR) is a toxin produced by freshwater cyanobacteria and is a potential threat to human health. MC-LR has been shown to be both a specific inhibitor of serine/threonine protein phosphatases PP1 and PP2A and a potent tumor promoter in rat liver. However, the genotoxic potential of MCs remains unclear. In this article, we investigated the ability of MC-LR to induce DNA damage on rat hepatocytes following intravenous (iv) administration by using two in vivo genotoxicity assays: the unscheduled DNA synthesis (UDS) and the comet assays. The UDS assay measures DNA synthesis induced from the excision repair of DNA damaged regions and the comet assay is a very sensitive technique for detecting various forms of DNA damage. After an exposure time of 2-4 h or 12-16 h and a dose ranging from 12.5 to 50 microg/kg bw, no DNA damage could be observed in both assays on rat hepatocytes following iv administration. These findings have been discussed and compared with recently published genotoxic results obtained in other organs from mice after oral and intraperitoneal treatments to better understand the mechanism of action of this toxin in relation with its cancerogenicity potential. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/18561296/In_vivo_genotoxic_potential_of_microcystin_LR:_a_cyanobacterial_toxin_investigated_both_by_the_unscheduled_DNA_synthesis__UDS__and_the_comet_assays_after_intravenous_administration_ L2 - https://doi.org/10.1002/tox.20417 DB - PRIME DP - Unbound Medicine ER -