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Assessment of VEGF-receptor system expression in the porcine endometrial stromal cells in response to insulin-like growth factor-I, relaxin, oxytocin and prostaglandin E2.
Mol Cell Endocrinol. 2008 Sep 10; 291(1-2):33-41.MC

Abstract

Several factors participate in regulation of growth and development as well as angiogenesis of the uterus during pregnancy, and hence little is known about the role of hormonal regulation of vascular endothelial growth factor (VEGF)-receptor system expression. This study has examined the effect of insulin-like growth factor-I (IGF-I), relaxin (RLX), oxytocin (OT) and prostaglandin (PG) E(2), on VEGF secretion and VEGF-receptor system mRNA expression in the porcine endometrial stromal cells. IGF-I and RLX were identified as the most effective inducers of VEGF secretion and mRNA expression. Although PGE(2) stimulated VEGF secretion and VEGF164 mRNA expression, OT inhibited both secretion and mRNA expression of VEGF. When tested for VEGF receptors (R), all factors failed to affect their mRNA expression. Media conditioned by stromal cells collected after IGF-I and RLX treatment significantly increased endothelial cell proliferation and this effect was blocked by soluble VEGFR-1. These data suggest that during early pregnancy IGF-I, RLX and PGE(2) can affect VEGF expression in the endometrium and therefore may support uterine and embryo development, implantation and pregnancy.

Authors+Show Affiliations

Division of Reproductive Endocrinology and Pathophysiology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland. moka@pan.olsztyn.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18562087

Citation

Kaczmarek, Monika M., et al. "Assessment of VEGF-receptor System Expression in the Porcine Endometrial Stromal Cells in Response to Insulin-like Growth factor-I, Relaxin, Oxytocin and Prostaglandin E2." Molecular and Cellular Endocrinology, vol. 291, no. 1-2, 2008, pp. 33-41.
Kaczmarek MM, Blitek A, Kaminska K, et al. Assessment of VEGF-receptor system expression in the porcine endometrial stromal cells in response to insulin-like growth factor-I, relaxin, oxytocin and prostaglandin E2. Mol Cell Endocrinol. 2008;291(1-2):33-41.
Kaczmarek, M. M., Blitek, A., Kaminska, K., Bodek, G., Zygmunt, M., Schams, D., & Ziecik, A. J. (2008). Assessment of VEGF-receptor system expression in the porcine endometrial stromal cells in response to insulin-like growth factor-I, relaxin, oxytocin and prostaglandin E2. Molecular and Cellular Endocrinology, 291(1-2), 33-41. https://doi.org/10.1016/j.mce.2008.04.020
Kaczmarek MM, et al. Assessment of VEGF-receptor System Expression in the Porcine Endometrial Stromal Cells in Response to Insulin-like Growth factor-I, Relaxin, Oxytocin and Prostaglandin E2. Mol Cell Endocrinol. 2008 Sep 10;291(1-2):33-41. PubMed PMID: 18562087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of VEGF-receptor system expression in the porcine endometrial stromal cells in response to insulin-like growth factor-I, relaxin, oxytocin and prostaglandin E2. AU - Kaczmarek,Monika M, AU - Blitek,Agnieszka, AU - Kaminska,Katarzyna, AU - Bodek,Gabriel, AU - Zygmunt,Marek, AU - Schams,Dieter, AU - Ziecik,Adam J, Y1 - 2008/05/14/ PY - 2007/09/28/received PY - 2008/04/20/revised PY - 2008/04/28/accepted PY - 2008/6/20/pubmed PY - 2008/10/7/medline PY - 2008/6/20/entrez SP - 33 EP - 41 JF - Molecular and cellular endocrinology JO - Mol Cell Endocrinol VL - 291 IS - 1-2 N2 - Several factors participate in regulation of growth and development as well as angiogenesis of the uterus during pregnancy, and hence little is known about the role of hormonal regulation of vascular endothelial growth factor (VEGF)-receptor system expression. This study has examined the effect of insulin-like growth factor-I (IGF-I), relaxin (RLX), oxytocin (OT) and prostaglandin (PG) E(2), on VEGF secretion and VEGF-receptor system mRNA expression in the porcine endometrial stromal cells. IGF-I and RLX were identified as the most effective inducers of VEGF secretion and mRNA expression. Although PGE(2) stimulated VEGF secretion and VEGF164 mRNA expression, OT inhibited both secretion and mRNA expression of VEGF. When tested for VEGF receptors (R), all factors failed to affect their mRNA expression. Media conditioned by stromal cells collected after IGF-I and RLX treatment significantly increased endothelial cell proliferation and this effect was blocked by soluble VEGFR-1. These data suggest that during early pregnancy IGF-I, RLX and PGE(2) can affect VEGF expression in the endometrium and therefore may support uterine and embryo development, implantation and pregnancy. SN - 0303-7207 UR - https://www.unboundmedicine.com/medline/citation/18562087/Assessment_of_VEGF_receptor_system_expression_in_the_porcine_endometrial_stromal_cells_in_response_to_insulin_like_growth_factor_I_relaxin_oxytocin_and_prostaglandin_E2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0303-7207(08)00157-3 DB - PRIME DP - Unbound Medicine ER -