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The development of protein chip using protein G for the simultaneous detection of various pathogens.
Ultramicroscopy. 2008 Sep; 108(10):1396-400.U

Abstract

A protein chip using protein G for the simultaneous detection of various pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, Yersinia enterocolitica, and Legionella pneumophila was developed. In order to endow the orientation of antibody molecules on solid surface, protein G was introduced. The protein G on gold (Au) surface modified with 11-mercaptoundecanoic acid (MUA) was arrayed and then four different kinds of monoclonal antibodies (Mabs) against pathogens (E. coli O157:H7, S. typhimurium, Y. enterocolitica, and L. pneumophila) on protein G spots were selectively arrayed using a microarrayer, and its spatial density was over 2400 spots cm(2). Using the constructed protein chip, the various pathogens such as E. coli O157:H7, S. typhimurium, Y. enterocolitica, and L. pneumophila could be detected by a sandwich method and its lowest detection limit for E. coli O157:H7 was 10(2) CFU/ml. The proposed fabrication technique of protein chip for the detection of various pathogens could be applied to construct other protein chips with a high efficiency.

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Authors+Show Affiliations

Choi JW
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea.
Kim YK
No affiliation info available
Oh BK
No affiliation info available

MeSH

Antibodies, BacterialAntibodies, MonoclonalColony Count, MicrobialGoldGram-Negative BacteriaGram-Negative Bacterial InfectionsHumansImmunoassayMicroscopy, Atomic ForceNerve Tissue ProteinsProtein Array Analysis

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18562117

Citation

Choi, Jeong-Woo, et al. "The Development of Protein Chip Using Protein G for the Simultaneous Detection of Various Pathogens." Ultramicroscopy, vol. 108, no. 10, 2008, pp. 1396-400.
Choi JW, Kim YK, Oh BK. The development of protein chip using protein G for the simultaneous detection of various pathogens. Ultramicroscopy. 2008;108(10):1396-400.
Choi, J. W., Kim, Y. K., & Oh, B. K. (2008). The development of protein chip using protein G for the simultaneous detection of various pathogens. Ultramicroscopy, 108(10), 1396-400. https://doi.org/10.1016/j.ultramic.2008.04.050
Choi JW, Kim YK, Oh BK. The Development of Protein Chip Using Protein G for the Simultaneous Detection of Various Pathogens. Ultramicroscopy. 2008;108(10):1396-400. PubMed PMID: 18562117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The development of protein chip using protein G for the simultaneous detection of various pathogens. AU - Choi,Jeong-Woo, AU - Kim,Young-Kee, AU - Oh,Byung-Keun, Y1 - 2008/05/10/ PY - 2008/6/20/pubmed PY - 2008/12/17/medline PY - 2008/6/20/entrez SP - 1396 EP - 400 JF - Ultramicroscopy JO - Ultramicroscopy VL - 108 IS - 10 N2 - A protein chip using protein G for the simultaneous detection of various pathogens such as Escherichia coli O157:H7, Salmonella typhimurium, Yersinia enterocolitica, and Legionella pneumophila was developed. In order to endow the orientation of antibody molecules on solid surface, protein G was introduced. The protein G on gold (Au) surface modified with 11-mercaptoundecanoic acid (MUA) was arrayed and then four different kinds of monoclonal antibodies (Mabs) against pathogens (E. coli O157:H7, S. typhimurium, Y. enterocolitica, and L. pneumophila) on protein G spots were selectively arrayed using a microarrayer, and its spatial density was over 2400 spots cm(2). Using the constructed protein chip, the various pathogens such as E. coli O157:H7, S. typhimurium, Y. enterocolitica, and L. pneumophila could be detected by a sandwich method and its lowest detection limit for E. coli O157:H7 was 10(2) CFU/ml. The proposed fabrication technique of protein chip for the detection of various pathogens could be applied to construct other protein chips with a high efficiency. SN - 0304-3991 UR - https://www.unboundmedicine.com/medline/citation/18562117/The_development_of_protein_chip_using_protein_G_for_the_simultaneous_detection_of_various_pathogens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3991(08)00147-2 DB - PRIME DP - Unbound Medicine ER -