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Simultaneous determination of amphetamine-type stimulants and cannabinoids in fingernails by gas chromatography-mass spectrometry.
Arch Pharm Res. 2008 Jun; 31(6):805-13.AP

Abstract

A gas chromatography-mass spectrometric (GC-MS) method was developed and validated for the simultaneous detection and quantification of four amphetamine-type stimulants (amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA)) and two cannabinoids (Delta9-tetrahydrocannabinol (Delta9-THC) and 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid (THCCOOH)) in fingernails. Fingernail clippings (30 mg) were washed with distilled water and methanol, and then incubated in 1.0 M sodium hydroxide at 95 degrees C for 30 min. The compounds of interest were isolated by liquid-liquid extraction followed by derivatization with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) at 70 degrees C for 15 min. The derivatized compounds were analyzed by GC-MS in the selective ion monitoring (SIM) mode. The linear ranges were 0.1-15.0 ng/mg for AP, 0.2-15.0 ng/mg for MDA, Delta9-THC and THCCOOH, and 0.2-30.0 ng/mg for MA and MDMA, with good correlation coefficients (r2 > 0.9991). The intra-day, inter-day, and inter-person precisions were within 10.6%, 6.3%, and 5.3%, respectively. The intra-day, inter-day and inter-person accuracies were between -6.1 and 5.0%, -6.2 and 5.7%, and -6.4 and 5.6%, respectively. The limits of detection (LODs) and quantification (LOQs) for each compound were lower than 0.056 and 0.2 ng/mg, respectively. The recoveries were in the range of 74.0-94.8%. Positive GC-MS results were obtained from specimens of nine suspected MA or cannabis abusers. The concentration ranges of MA, AP, and THCCOOH were 0.10-1.41, 0.12-2.64, and 0.20 ng/mg, respectively. Based on these results, the method proved to be effective for the simultaneous qualification and quantification of amphetamine-type stimulants and cannabinoids in fingernails.

Authors+Show Affiliations

Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecutors' Office, 706, Banporo, Seocho-gu, Seoul, 137-730, Korea. paxus@spo.go.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18563365

Citation

Kim, Jin Young, et al. "Simultaneous Determination of Amphetamine-type Stimulants and Cannabinoids in Fingernails By Gas Chromatography-mass Spectrometry." Archives of Pharmacal Research, vol. 31, no. 6, 2008, pp. 805-13.
Kim JY, Cheong JC, Kim MK, et al. Simultaneous determination of amphetamine-type stimulants and cannabinoids in fingernails by gas chromatography-mass spectrometry. Arch Pharm Res. 2008;31(6):805-13.
Kim, J. Y., Cheong, J. C., Kim, M. K., Lee, J. I., & In, M. K. (2008). Simultaneous determination of amphetamine-type stimulants and cannabinoids in fingernails by gas chromatography-mass spectrometry. Archives of Pharmacal Research, 31(6), 805-13. https://doi.org/10.1007/s12272-001-1230-5
Kim JY, et al. Simultaneous Determination of Amphetamine-type Stimulants and Cannabinoids in Fingernails By Gas Chromatography-mass Spectrometry. Arch Pharm Res. 2008;31(6):805-13. PubMed PMID: 18563365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of amphetamine-type stimulants and cannabinoids in fingernails by gas chromatography-mass spectrometry. AU - Kim,Jin Young, AU - Cheong,Jae Chul, AU - Kim,Min Kyoung, AU - Lee,Jae Il, AU - In,Moon Kyo, Y1 - 2008/06/19/ PY - 2008/02/12/received PY - 2008/04/15/accepted PY - 2008/03/10/revised PY - 2008/6/20/pubmed PY - 2008/9/20/medline PY - 2008/6/20/entrez SP - 805 EP - 13 JF - Archives of pharmacal research JO - Arch. Pharm. Res. VL - 31 IS - 6 N2 - A gas chromatography-mass spectrometric (GC-MS) method was developed and validated for the simultaneous detection and quantification of four amphetamine-type stimulants (amphetamine (AP), methamphetamine (MA), 3,4-methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA)) and two cannabinoids (Delta9-tetrahydrocannabinol (Delta9-THC) and 11-nor-Delta9-tetrahydrocannabinol-9-carboxylic acid (THCCOOH)) in fingernails. Fingernail clippings (30 mg) were washed with distilled water and methanol, and then incubated in 1.0 M sodium hydroxide at 95 degrees C for 30 min. The compounds of interest were isolated by liquid-liquid extraction followed by derivatization with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) at 70 degrees C for 15 min. The derivatized compounds were analyzed by GC-MS in the selective ion monitoring (SIM) mode. The linear ranges were 0.1-15.0 ng/mg for AP, 0.2-15.0 ng/mg for MDA, Delta9-THC and THCCOOH, and 0.2-30.0 ng/mg for MA and MDMA, with good correlation coefficients (r2 > 0.9991). The intra-day, inter-day, and inter-person precisions were within 10.6%, 6.3%, and 5.3%, respectively. The intra-day, inter-day and inter-person accuracies were between -6.1 and 5.0%, -6.2 and 5.7%, and -6.4 and 5.6%, respectively. The limits of detection (LODs) and quantification (LOQs) for each compound were lower than 0.056 and 0.2 ng/mg, respectively. The recoveries were in the range of 74.0-94.8%. Positive GC-MS results were obtained from specimens of nine suspected MA or cannabis abusers. The concentration ranges of MA, AP, and THCCOOH were 0.10-1.41, 0.12-2.64, and 0.20 ng/mg, respectively. Based on these results, the method proved to be effective for the simultaneous qualification and quantification of amphetamine-type stimulants and cannabinoids in fingernails. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/18563365/Simultaneous_determination_of_amphetamine_type_stimulants_and_cannabinoids_in_fingernails_by_gas_chromatography_mass_spectrometry_ L2 - https://dx.doi.org/10.1007/s12272-001-1230-5 DB - PRIME DP - Unbound Medicine ER -