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Chromosomal location of Lg-FLO1 in bottom-fermenting yeast and the FLO5 locus of industrial yeast.
J Appl Microbiol. 2008 Oct; 105(4):1186-98.JA

Abstract

AIMS

To determine the chromosomal location and entire sequence of Lg-FLO1, the expression of which causes the flocculation of bottom-fermenting yeast.

METHODS AND RESULTS

Two cosmid clones carrying DNA from a bottom-fermenting yeast chromosome VIII right-arm end were selected by colony hybridization. Sequencing revealed that the clones contained DNA derived from a Saccharomyces cerevisiae type chromosome VIII and a Saccharomyces bayanus type chromosome VIII, both from bottom-fermenting yeast.

CONCLUSIONS

Lg-FLO1 is located on the S. cerevisiae type chromosome VIII at the same position as the FLO5 gene of the laboratory yeast S. cerevisiae S288c. The unique chromosome VIII structure of bottom-fermenting yeast is conserved among other related strains. FLO5 and Lg-FLO1 promoter sequences are identical except for the presence of three 42 bp repeats in the latter, which are associated with gene activity. Flocculin genes might have been generated by chromosomal recombination at these repeats.

SIGNIFICANCE AND IMPACT OF THE STUDY

This is the first report of the exact chromosomal location and entire sequence of Lg-FLO1. This information will be useful in the brewing industry for the identification of normal bottom-fermenting yeast. Moreover, variations in the FLO5 locus among strains are thought to reflect yeast evolution.

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Authors+Show Affiliations

Ogata T
Brewing Research & Development Laboratory, Asahi Breweries Ltd., Ibaraki, Japan. tomoo.ogata@asahibeer.co.jp
Izumikawa M
No affiliation info available
Kohno K
No affiliation info available
Shibata K
No affiliation info available

MeSH

Base SequenceBeerChromosome MappingCloning, MolecularDNA PrimersFlocculationGenes, FungalIndustrial MicrobiologyMannose-Binding LectinsMolecular Sequence DataPhylogenyPolymerase Chain ReactionSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSequence Analysis, DNASequence Homology

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18564345

Citation

Ogata, T, et al. "Chromosomal Location of Lg-FLO1 in Bottom-fermenting Yeast and the FLO5 Locus of Industrial Yeast." Journal of Applied Microbiology, vol. 105, no. 4, 2008, pp. 1186-98.
Ogata T, Izumikawa M, Kohno K, et al. Chromosomal location of Lg-FLO1 in bottom-fermenting yeast and the FLO5 locus of industrial yeast. J Appl Microbiol. 2008;105(4):1186-98.
Ogata, T., Izumikawa, M., Kohno, K., & Shibata, K. (2008). Chromosomal location of Lg-FLO1 in bottom-fermenting yeast and the FLO5 locus of industrial yeast. Journal of Applied Microbiology, 105(4), 1186-98. https://doi.org/10.1111/j.1365-2672.2008.03852.x
Ogata T, et al. Chromosomal Location of Lg-FLO1 in Bottom-fermenting Yeast and the FLO5 Locus of Industrial Yeast. J Appl Microbiol. 2008;105(4):1186-98. PubMed PMID: 18564345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosomal location of Lg-FLO1 in bottom-fermenting yeast and the FLO5 locus of industrial yeast. AU - Ogata,T, AU - Izumikawa,M, AU - Kohno,K, AU - Shibata,K, Y1 - 2008/06/28/ PY - 2008/6/20/pubmed PY - 2008/12/25/medline PY - 2008/6/20/entrez SP - 1186 EP - 98 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 105 IS - 4 N2 - AIMS: To determine the chromosomal location and entire sequence of Lg-FLO1, the expression of which causes the flocculation of bottom-fermenting yeast. METHODS AND RESULTS: Two cosmid clones carrying DNA from a bottom-fermenting yeast chromosome VIII right-arm end were selected by colony hybridization. Sequencing revealed that the clones contained DNA derived from a Saccharomyces cerevisiae type chromosome VIII and a Saccharomyces bayanus type chromosome VIII, both from bottom-fermenting yeast. CONCLUSIONS: Lg-FLO1 is located on the S. cerevisiae type chromosome VIII at the same position as the FLO5 gene of the laboratory yeast S. cerevisiae S288c. The unique chromosome VIII structure of bottom-fermenting yeast is conserved among other related strains. FLO5 and Lg-FLO1 promoter sequences are identical except for the presence of three 42 bp repeats in the latter, which are associated with gene activity. Flocculin genes might have been generated by chromosomal recombination at these repeats. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of the exact chromosomal location and entire sequence of Lg-FLO1. This information will be useful in the brewing industry for the identification of normal bottom-fermenting yeast. Moreover, variations in the FLO5 locus among strains are thought to reflect yeast evolution. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/18564345/Chromosomal_location_of_Lg_FLO1_in_bottom_fermenting_yeast_and_the_FLO5_locus_of_industrial_yeast_ L2 - https://doi.org/10.1111/j.1365-2672.2008.03852.x DB - PRIME DP - Unbound Medicine ER -