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Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II.
J Infect Dis. 1991 Aug; 164(2):400-3.JI

Abstract

Disease association studies of human T cell lymphotropic virus (HTLV) types I and II are hindered by the need for multiple assays to confirm and differentiate between the viruses. A modified Western blot assay has been developed using HTLV-I viral lysate and unique (MTA-4) and shared (p21E) HTLV recombinant proteins. By defining confirmation of infection as the presence of antibodies to p24 gag protein and to p21E, all 56 HTLV-I and 49 HTLV-II antisera were confirmed by this modified Western blot alone. Differentiation was determined by reactivity to MTA-4. All HTLV-I antisera reacted with MTA-4 and all HTLV-II antisera did not react with MTA-4. These findings indicate the utility of selected HTLV-I recombinant proteins in a single assay format to confirm and differentiate infections with HTLV-I and HTLV-II.

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Authors+Show Affiliations

Lipka JJ
Department of Pathology, Stanford University School of Medicine, Palo Alto, CA.
Santiago P
No affiliation info available
Chan L
No affiliation info available
Reyes GR
No affiliation info available
Samuel KP
No affiliation info available
Blattner WA
No affiliation info available
Shaw GM
No affiliation info available
Hanson CV
No affiliation info available
Sninsky JJ
No affiliation info available
Foung SK
No affiliation info available

MeSH

Blotting, WesternFalse Positive ReactionsHTLV-I AntibodiesHTLV-I InfectionsHTLV-II AntibodiesHTLV-II InfectionsHuman T-lymphotropic virus 1Human T-lymphotropic virus 2HumansImmune SeraRadioimmunoprecipitation Assay

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1856487

Citation

Lipka, J J., et al. "Modified Western Blot Assay for Confirmation and Differentiation of Human T Cell Lymphotropic Virus Types I and II." The Journal of Infectious Diseases, vol. 164, no. 2, 1991, pp. 400-3.
Lipka JJ, Santiago P, Chan L, et al. Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II. J Infect Dis. 1991;164(2):400-3.
Lipka, J. J., Santiago, P., Chan, L., Reyes, G. R., Samuel, K. P., Blattner, W. A., Shaw, G. M., Hanson, C. V., Sninsky, J. J., & Foung, S. K. (1991). Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II. The Journal of Infectious Diseases, 164(2), 400-3.
Lipka JJ, et al. Modified Western Blot Assay for Confirmation and Differentiation of Human T Cell Lymphotropic Virus Types I and II. J Infect Dis. 1991;164(2):400-3. PubMed PMID: 1856487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II. AU - Lipka,J J, AU - Santiago,P, AU - Chan,L, AU - Reyes,G R, AU - Samuel,K P, AU - Blattner,W A, AU - Shaw,G M, AU - Hanson,C V, AU - Sninsky,J J, AU - Foung,S K, PY - 1991/8/1/pubmed PY - 1991/8/1/medline PY - 1991/8/1/entrez SP - 400 EP - 3 JF - The Journal of infectious diseases JO - J Infect Dis VL - 164 IS - 2 N2 - Disease association studies of human T cell lymphotropic virus (HTLV) types I and II are hindered by the need for multiple assays to confirm and differentiate between the viruses. A modified Western blot assay has been developed using HTLV-I viral lysate and unique (MTA-4) and shared (p21E) HTLV recombinant proteins. By defining confirmation of infection as the presence of antibodies to p24 gag protein and to p21E, all 56 HTLV-I and 49 HTLV-II antisera were confirmed by this modified Western blot alone. Differentiation was determined by reactivity to MTA-4. All HTLV-I antisera reacted with MTA-4 and all HTLV-II antisera did not react with MTA-4. These findings indicate the utility of selected HTLV-I recombinant proteins in a single assay format to confirm and differentiate infections with HTLV-I and HTLV-II. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/1856487/Modified_Western_blot_assay_for_confirmation_and_differentiation_of_human_T_cell_lymphotropic_virus_types_I_and_II_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/164.2.400 DB - PRIME DP - Unbound Medicine ER -