Tags

Type your tag names separated by a space and hit enter

Metabolic analysis of body fluids by capillary electrophoresis using noncovalently coated capillaries.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 15; 871(2):370-4.JC

Abstract

The potential of capillaries noncovalently coated with charged polymers for the metabolic analysis of body fluids by CE is illustrated. Firstly, the usefulness of a coating consisting of a triple layer of polybrene-dextran sulfate-polybrene for the fast analysis of organic acids is described. The CE system allowed direct injections of CSF, plasma and urine samples, yielding good separation efficiencies. RSDs for migration times and peak areas of organic acids in plasma were <3% and <5%, respectively. The usefulness of the system is illustrated by the profiling of organic acids in plasma and urine samples. Secondly, a CE system comprising a bilayer coating of polybrene-poly(vinylsulfonate), which provides a considerable EOF at low pH is described. This system was combined with TOF-MS and used for the fast analysis of amino acids in cerebrospinal fluid (CSF) and urine with minimal sample pretreatment. RSDs for migration times and peak areas of amino acids in CSF and urine were <2% and <10%, respectively. The applicability of the system is demonstrated by the profiling of endogenous low-molecular weight metabolites in CSF from a healthy individual and a patient with complex regional pain syndrome.

Links

Publisher Full Text

Authors+Show Affiliations

Ramautar R
Department of Biomedical Analysis, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands. R.Ramautar@uu.nl
Mayboroda OA
No affiliation info available
Deelder AM
No affiliation info available
Somsen GW
No affiliation info available
de Jong GJ
No affiliation info available

MeSH

Body FluidsChromatography, Micellar Electrokinetic CapillaryElectrophoresis, CapillaryHumansMass SpectrometryReproducibility of Results

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18565806

Citation

Ramautar, Rawi, et al. "Metabolic Analysis of Body Fluids By Capillary Electrophoresis Using Noncovalently Coated Capillaries." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 871, no. 2, 2008, pp. 370-4.
Ramautar R, Mayboroda OA, Deelder AM, et al. Metabolic analysis of body fluids by capillary electrophoresis using noncovalently coated capillaries. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;871(2):370-4.
Ramautar, R., Mayboroda, O. A., Deelder, A. M., Somsen, G. W., & de Jong, G. J. (2008). Metabolic analysis of body fluids by capillary electrophoresis using noncovalently coated capillaries. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 871(2), 370-4. https://doi.org/10.1016/j.jchromb.2008.06.004
Ramautar R, et al. Metabolic Analysis of Body Fluids By Capillary Electrophoresis Using Noncovalently Coated Capillaries. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 15;871(2):370-4. PubMed PMID: 18565806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic analysis of body fluids by capillary electrophoresis using noncovalently coated capillaries. AU - Ramautar,Rawi, AU - Mayboroda,Oleg A, AU - Deelder,André M, AU - Somsen,Govert W, AU - de Jong,Gerhardus J, Y1 - 2008/06/06/ PY - 2008/03/28/received PY - 2008/05/21/revised PY - 2008/06/02/accepted PY - 2008/6/21/pubmed PY - 2008/10/10/medline PY - 2008/6/21/entrez SP - 370 EP - 4 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 871 IS - 2 N2 - The potential of capillaries noncovalently coated with charged polymers for the metabolic analysis of body fluids by CE is illustrated. Firstly, the usefulness of a coating consisting of a triple layer of polybrene-dextran sulfate-polybrene for the fast analysis of organic acids is described. The CE system allowed direct injections of CSF, plasma and urine samples, yielding good separation efficiencies. RSDs for migration times and peak areas of organic acids in plasma were <3% and <5%, respectively. The usefulness of the system is illustrated by the profiling of organic acids in plasma and urine samples. Secondly, a CE system comprising a bilayer coating of polybrene-poly(vinylsulfonate), which provides a considerable EOF at low pH is described. This system was combined with TOF-MS and used for the fast analysis of amino acids in cerebrospinal fluid (CSF) and urine with minimal sample pretreatment. RSDs for migration times and peak areas of amino acids in CSF and urine were <2% and <10%, respectively. The applicability of the system is demonstrated by the profiling of endogenous low-molecular weight metabolites in CSF from a healthy individual and a patient with complex regional pain syndrome. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18565806/Metabolic_analysis_of_body_fluids_by_capillary_electrophoresis_using_noncovalently_coated_capillaries_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00399-1 DB - PRIME DP - Unbound Medicine ER -