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Essential role of NOXA1 in generation of reactive oxygen species induced by oxidized low-density lipoprotein in human vascular endothelial cells.
Endothelium. 2008 May-Jun; 15(3):137-41.E

Abstract

Oxidative stress induced by superoxide plays an important role in pathogenesis of cardiovascular diseases. NAD(P)H oxidase is a principal enzymatic origin for superoxide in vasculature. Recently, novel homologues of cytosolic components of NAD(P)H oxidase, Nox organizer 1 (NOXO1) and Nox activator 1 (NOXA1), are identified. On the other hand, oxidized low-density lipoprotein (ox-LDL) generates reactive oxygen species (ROS) in endothelial cells via lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). In the present investigation, the authors examined the expression, the regulation, and the role of NOXA1 in the generation of ROS in endothelial cells. The expression of NOXA1 was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR). Dihydroethidium method showed that ox-LDL and angiotensin II increased the generation of intracellular ROS. Once the expression of p22(phox) or NOXA1 was suppressed by siRNA, the generation of ROS induced by ox-LDL and angiotensin II were potently decreased. Moreover, the expression of NOXA1 was increased by ox-LDL in a time-and dose-dependent manner. In conclusion, endothelial NOXA1 plays an essential role in generation of ROS. Ox-LDL not only increased the generation of ROS via LOX-1, but also enhanced the expression of NOXA1 in endothelial cells. NOXA1 is likely a key player that links ox-LDL with the activation of endothelial NAD(P)H oxidase.

Authors+Show Affiliations

Division of Cardiovascular and Respiratory Medicine, Department of Internal Medicine, Kobe University Graduate School of Medicine, Kobe, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18568954

Citation

Honjo, Tomoyuki, et al. "Essential Role of NOXA1 in Generation of Reactive Oxygen Species Induced By Oxidized Low-density Lipoprotein in Human Vascular Endothelial Cells." Endothelium : Journal of Endothelial Cell Research, vol. 15, no. 3, 2008, pp. 137-41.
Honjo T, Otsui K, Shiraki R, et al. Essential role of NOXA1 in generation of reactive oxygen species induced by oxidized low-density lipoprotein in human vascular endothelial cells. Endothelium. 2008;15(3):137-41.
Honjo, T., Otsui, K., Shiraki, R., Kawashima, S., Sawamura, T., Yokoyama, M., & Inoue, N. (2008). Essential role of NOXA1 in generation of reactive oxygen species induced by oxidized low-density lipoprotein in human vascular endothelial cells. Endothelium : Journal of Endothelial Cell Research, 15(3), 137-41. https://doi.org/10.1080/10623320802125433
Honjo T, et al. Essential Role of NOXA1 in Generation of Reactive Oxygen Species Induced By Oxidized Low-density Lipoprotein in Human Vascular Endothelial Cells. Endothelium. 2008 May-Jun;15(3):137-41. PubMed PMID: 18568954.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Essential role of NOXA1 in generation of reactive oxygen species induced by oxidized low-density lipoprotein in human vascular endothelial cells. AU - Honjo,Tomoyuki, AU - Otsui,Kazunori, AU - Shiraki,Rio, AU - Kawashima,Seinosuke, AU - Sawamura,Tatsuya, AU - Yokoyama,Mitsuhiro, AU - Inoue,Nobutaka, PY - 2008/6/24/pubmed PY - 2008/8/7/medline PY - 2008/6/24/entrez SP - 137 EP - 41 JF - Endothelium : journal of endothelial cell research JO - Endothelium VL - 15 IS - 3 N2 - Oxidative stress induced by superoxide plays an important role in pathogenesis of cardiovascular diseases. NAD(P)H oxidase is a principal enzymatic origin for superoxide in vasculature. Recently, novel homologues of cytosolic components of NAD(P)H oxidase, Nox organizer 1 (NOXO1) and Nox activator 1 (NOXA1), are identified. On the other hand, oxidized low-density lipoprotein (ox-LDL) generates reactive oxygen species (ROS) in endothelial cells via lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). In the present investigation, the authors examined the expression, the regulation, and the role of NOXA1 in the generation of ROS in endothelial cells. The expression of NOXA1 was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR). Dihydroethidium method showed that ox-LDL and angiotensin II increased the generation of intracellular ROS. Once the expression of p22(phox) or NOXA1 was suppressed by siRNA, the generation of ROS induced by ox-LDL and angiotensin II were potently decreased. Moreover, the expression of NOXA1 was increased by ox-LDL in a time-and dose-dependent manner. In conclusion, endothelial NOXA1 plays an essential role in generation of ROS. Ox-LDL not only increased the generation of ROS via LOX-1, but also enhanced the expression of NOXA1 in endothelial cells. NOXA1 is likely a key player that links ox-LDL with the activation of endothelial NAD(P)H oxidase. SN - 1029-2373 UR - https://www.unboundmedicine.com/medline/citation/18568954/Essential_role_of_NOXA1_in_generation_of_reactive_oxygen_species_induced_by_oxidized_low_density_lipoprotein_in_human_vascular_endothelial_cells_ DB - PRIME DP - Unbound Medicine ER -