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Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB).
J Invertebr Pathol. 2008 Sep; 99(1):8-13.JI

Abstract

A novel micro-PCR-based detection method, termed ultra-rapid real-time PCR, was applied to the development of a rapid detection for Paenibacillus larvae (P. larvae) which is the causative agent of American Foulbrood (AFB). This method was designed to detect the 16S rRNA gene of P. larvae with a micro-scale chip-based real-time PCR system, GenSpector TMC-1000, which has uncommonly fast heating and cooling rates (10 degrees C per second) and small reaction volume (6microl). In the application of ultra-rapid real-time PCR detection to an AFB-infected larva, the minimum detection time was 7 min and 54s total reaction time (30 cycles), including the melting temperature analysis. To the best of our knowledge, this novel detection method is one of the most rapid real-time PCR-based detection tools.

Authors+Show Affiliations

Department of Life Science, College of Natural Science, Kyonggi University, Suwon, Kyonggi-do, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18571197

Citation

Han, Sang-Hoon, et al. "Ultra-rapid Real-time PCR for the Detection of Paenibacillus Larvae, the Causative Agent of American Foulbrood (AFB)." Journal of Invertebrate Pathology, vol. 99, no. 1, 2008, pp. 8-13.
Han SH, Lee DB, Lee DW, et al. Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB). J Invertebr Pathol. 2008;99(1):8-13.
Han, S. H., Lee, D. B., Lee, D. W., Kim, E. H., & Yoon, B. S. (2008). Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB). Journal of Invertebrate Pathology, 99(1), 8-13. https://doi.org/10.1016/j.jip.2008.04.010
Han SH, et al. Ultra-rapid Real-time PCR for the Detection of Paenibacillus Larvae, the Causative Agent of American Foulbrood (AFB). J Invertebr Pathol. 2008;99(1):8-13. PubMed PMID: 18571197.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB). AU - Han,Sang-Hoon, AU - Lee,Do-Bu, AU - Lee,Dong-Woo, AU - Kim,Eul-Hwan, AU - Yoon,Byoung-Su, Y1 - 2008/05/06/ PY - 2007/08/09/received PY - 2008/04/24/revised PY - 2008/04/29/accepted PY - 2008/6/24/pubmed PY - 2008/11/7/medline PY - 2008/6/24/entrez SP - 8 EP - 13 JF - Journal of invertebrate pathology JO - J. Invertebr. Pathol. VL - 99 IS - 1 N2 - A novel micro-PCR-based detection method, termed ultra-rapid real-time PCR, was applied to the development of a rapid detection for Paenibacillus larvae (P. larvae) which is the causative agent of American Foulbrood (AFB). This method was designed to detect the 16S rRNA gene of P. larvae with a micro-scale chip-based real-time PCR system, GenSpector TMC-1000, which has uncommonly fast heating and cooling rates (10 degrees C per second) and small reaction volume (6microl). In the application of ultra-rapid real-time PCR detection to an AFB-infected larva, the minimum detection time was 7 min and 54s total reaction time (30 cycles), including the melting temperature analysis. To the best of our knowledge, this novel detection method is one of the most rapid real-time PCR-based detection tools. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/18571197/Ultra_rapid_real_time_PCR_for_the_detection_of_Paenibacillus_larvae_the_causative_agent_of_American_Foulbrood__AFB__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(08)00104-3 DB - PRIME DP - Unbound Medicine ER -