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Multicenter prospective study of the humoral autoimmune response in bullous pemphigoid.
Clin Immunol. 2008 Sep; 128(3):415-26.CI

Abstract

Bullous pemphigoid (BP) is an autoimmune bullous disease, associated with autoantibodies directed against the hemidesmosomal components BP180 and BP230. In this study for the first time different laboratories have analyzed the autoantibody profile in the same group of 49 prospectively recruited BP patients. The results show that: 1) disease severity and activity correlated with levels of IgG against the BP180-NC16A domain, but also against a COOH-terminal epitope of BP180, 2) distinct epitopes of the BP180 ectodomain other than BP180-NC16A were recognized by 96% of the BP sera; and 3) the combined use of BP180 and BP230 ELISA led to the detection of IgG autoantibodies in all the BP sera. These results demonstrate the usefulness of the combined ELISAs based on various BP180 and BP230 fragments in establishing the diagnosis of BP and support the concept that BP180 is the major autoantigen of BP.

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Authors+Show Affiliations

Di Zenzo G
Istituto Dermopatico dell'Immacolata, Molecular and Cell Biology Laboratory, IDI-IRCCS, Rome, Italy. g.dizenzo@idi.it
Thoma-Uszynski S
No affiliation info available
Fontao L
No affiliation info available
Calabresi V
No affiliation info available
Hofmann SC
No affiliation info available
Hellmark T
No affiliation info available
Sebbag N
No affiliation info available
Pedicelli C
No affiliation info available
Sera F
No affiliation info available
Lacour JP
No affiliation info available
Wieslander J
No affiliation info available
Bruckner-Tuderman L
No affiliation info available
Borradori L
No affiliation info available
Zambruno G
No affiliation info available
Hertl M
No affiliation info available

MeSH

AgedAged, 80 and overAutoantibodiesAutoantigensEpitopesFemaleHumansImmunoglobulin AImmunoglobulin GMaleMiddle AgedNon-Fibrillar CollagensPemphigoid, BullousProspective StudiesRecombinant Proteins

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18571472

Citation

Di Zenzo, Giovanni, et al. "Multicenter Prospective Study of the Humoral Autoimmune Response in Bullous Pemphigoid." Clinical Immunology (Orlando, Fla.), vol. 128, no. 3, 2008, pp. 415-26.
Di Zenzo G, Thoma-Uszynski S, Fontao L, et al. Multicenter prospective study of the humoral autoimmune response in bullous pemphigoid. Clin Immunol. 2008;128(3):415-26.
Di Zenzo, G., Thoma-Uszynski, S., Fontao, L., Calabresi, V., Hofmann, S. C., Hellmark, T., Sebbag, N., Pedicelli, C., Sera, F., Lacour, J. P., Wieslander, J., Bruckner-Tuderman, L., Borradori, L., Zambruno, G., & Hertl, M. (2008). Multicenter prospective study of the humoral autoimmune response in bullous pemphigoid. Clinical Immunology (Orlando, Fla.), 128(3), 415-26. https://doi.org/10.1016/j.clim.2008.04.012
Di Zenzo G, et al. Multicenter Prospective Study of the Humoral Autoimmune Response in Bullous Pemphigoid. Clin Immunol. 2008;128(3):415-26. PubMed PMID: 18571472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multicenter prospective study of the humoral autoimmune response in bullous pemphigoid. AU - Di Zenzo,Giovanni, AU - Thoma-Uszynski,Sybille, AU - Fontao,Lionel, AU - Calabresi,Valentina, AU - Hofmann,Silke C, AU - Hellmark,Thomas, AU - Sebbag,Nathalie, AU - Pedicelli,Cristina, AU - Sera,Francesco, AU - Lacour,Jean-Philippe, AU - Wieslander,Jörgen, AU - Bruckner-Tuderman,Leena, AU - Borradori,Luca, AU - Zambruno,Giovanna, AU - Hertl,Michael, Y1 - 2008/06/20/ PY - 2008/02/13/received PY - 2008/04/28/revised PY - 2008/04/29/accepted PY - 2008/6/24/pubmed PY - 2008/9/6/medline PY - 2008/6/24/entrez SP - 415 EP - 26 JF - Clinical immunology (Orlando, Fla.) JO - Clin Immunol VL - 128 IS - 3 N2 - Bullous pemphigoid (BP) is an autoimmune bullous disease, associated with autoantibodies directed against the hemidesmosomal components BP180 and BP230. In this study for the first time different laboratories have analyzed the autoantibody profile in the same group of 49 prospectively recruited BP patients. The results show that: 1) disease severity and activity correlated with levels of IgG against the BP180-NC16A domain, but also against a COOH-terminal epitope of BP180, 2) distinct epitopes of the BP180 ectodomain other than BP180-NC16A were recognized by 96% of the BP sera; and 3) the combined use of BP180 and BP230 ELISA led to the detection of IgG autoantibodies in all the BP sera. These results demonstrate the usefulness of the combined ELISAs based on various BP180 and BP230 fragments in establishing the diagnosis of BP and support the concept that BP180 is the major autoantigen of BP. SN - 1521-7035 UR - https://www.unboundmedicine.com/medline/citation/18571472/Multicenter_prospective_study_of_the_humoral_autoimmune_response_in_bullous_pemphigoid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(08)00651-7 DB - PRIME DP - Unbound Medicine ER -