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Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei.
Phytochemistry. 2008 Aug; 69(11):2193-9.P

Abstract

Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 microg/ml were much lower than the cytotoxic concentration 50% (CC50) values (1000 microg/ml). These fractions had very low anticoagulant activity. Furthermore, they had a weak inactivating effect on virions in a virucidal assay at concentrations in the range of 60-100 microg/ml. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide, which had 0.4 sulfate group per monomer unit and an apparent molecular mass of 160 kDa, contained a backbone of alpha-(1-->3)-linked D-mannopyranosyl residues substituted at C-6, C-4 and C-2 with single stub of beta-d-xylopyranosyl residues. Sulfate groups, when present, are located at C-4 of alpha-(1-->3)-linked D-mannopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer.

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Authors+Show Affiliations

Mandal P
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, West Bengal 713 104, India.
Pujol CA
No affiliation info available
Carlucci MJ
No affiliation info available
Chattopadhyay K
No affiliation info available
Damonte EB
No affiliation info available
Ray B
No affiliation info available

MeSH

AnimalsAnticoagulantsAntiviral AgentsChlorocebus aethiopsChromatography, GelGlycosidesHerpesvirus 1, HumanHerpesvirus 2, HumanHumansMagnetic Resonance SpectroscopyMannansRhodophytaSulfatesVero Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18572208

Citation

Mandal, Pinaki, et al. "Anti-herpetic Activity of a Sulfated Xylomannan From Scinaia Hatei." Phytochemistry, vol. 69, no. 11, 2008, pp. 2193-9.
Mandal P, Pujol CA, Carlucci MJ, et al. Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei. Phytochemistry. 2008;69(11):2193-9.
Mandal, P., Pujol, C. A., Carlucci, M. J., Chattopadhyay, K., Damonte, E. B., & Ray, B. (2008). Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei. Phytochemistry, 69(11), 2193-9. https://doi.org/10.1016/j.phytochem.2008.05.004
Mandal P, et al. Anti-herpetic Activity of a Sulfated Xylomannan From Scinaia Hatei. Phytochemistry. 2008;69(11):2193-9. PubMed PMID: 18572208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-herpetic activity of a sulfated xylomannan from Scinaia hatei. AU - Mandal,Pinaki, AU - Pujol,Carlos A, AU - Carlucci,María J, AU - Chattopadhyay,Kausik, AU - Damonte,Elsa B, AU - Ray,Bimalendu, Y1 - 2008/06/21/ PY - 2007/12/24/received PY - 2008/05/05/revised PY - 2008/05/07/accepted PY - 2008/6/24/pubmed PY - 2008/9/18/medline PY - 2008/6/24/entrez SP - 2193 EP - 9 JF - Phytochemistry JO - Phytochemistry VL - 69 IS - 11 N2 - Many viruses display affinity for cell surface heparan sulfate proteoglycans with biological relevance in virus entry. This raises the possibility of the application of sulfated polysaccharides in antiviral therapy. In this study we have analyzed polysaccharide fractions isolated from Scinaia hatei. The crude water extract (ShWE) as well as one fraction (F1) obtained by size exclusion chromatography had potent anti-HSV activity. Their inhibitory concentration 50% (IC50) values ranging from 0.5 to 4.6 microg/ml were much lower than the cytotoxic concentration 50% (CC50) values (1000 microg/ml). These fractions had very low anticoagulant activity. Furthermore, they had a weak inactivating effect on virions in a virucidal assay at concentrations in the range of 60-100 microg/ml. Chemical, chromatographic and spectroscopic methods showed that the major polysaccharide, which had 0.4 sulfate group per monomer unit and an apparent molecular mass of 160 kDa, contained a backbone of alpha-(1-->3)-linked D-mannopyranosyl residues substituted at C-6, C-4 and C-2 with single stub of beta-d-xylopyranosyl residues. Sulfate groups, when present, are located at C-4 of alpha-(1-->3)-linked D-mannopyranosyl units, and appeared to be very important for the anti-herpetic activity of this polymer. SN - 0031-9422 UR - https://www.unboundmedicine.com/medline/citation/18572208/Anti_herpetic_activity_of_a_sulfated_xylomannan_from_Scinaia_hatei_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(08)00227-6 DB - PRIME DP - Unbound Medicine ER -