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Distribution of Paenibacillus larvae spores inside honey bee colonies and its relevance for diagnosis.
J Invertebr Pathol. 2008 Sep; 99(1):92-5.JI

Abstract

One of the most important factors affecting the development of honey bee colonies is infectious diseases such as American foulbrood (AFB) caused by the spore forming Gram-positive bacterium Paenibacillus larvae. Colony inspections for AFB clinical symptoms are time consuming. Moreover, diseased cells in the early stages of the infection may easily be overlooked. In this study, we investigated whether it is possible to determine the sanitary status of a colony based on analyses of different materials collected from the hive. We analysed 237 bee samples and 67 honey samples originating from 71 colonies situated in 13 apiaries with clinical AFB occurrences. We tested whether a difference in spore load among bees inside the whole hive exists and which sample material related to its location inside the hive was the most appropriate for an early AFB diagnosis based on the culture method. Results indicated that diagnostics based on analysis of honey samples and bees collected at the hive entrance are of limited value as only 86% and 83%, respectively, of samples from AFB-symptomatic colonies were positive. Analysis of bee samples collected from the brood nest, honey chamber, and edge frame allowed the detection of all colonies showing AFB clinical symptoms. Microbiological analysis showed that more than one quarter of samples collected from colonies without AFB clinical symptoms were positive for P. larvae. Based on these results, we recommend investigating colonies by testing bee samples from the brood nest, edge frame or honey chamber for P. larvae spores.

Authors+Show Affiliations

Institut Galli Valerio, Veterinary Diagnostic Laboratory of the Canton Vaud, Lausanne, Switzerland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18573258

Citation

Gillard, M, et al. "Distribution of Paenibacillus Larvae Spores Inside Honey Bee Colonies and Its Relevance for Diagnosis." Journal of Invertebrate Pathology, vol. 99, no. 1, 2008, pp. 92-5.
Gillard M, Charriere JD, Belloy L. Distribution of Paenibacillus larvae spores inside honey bee colonies and its relevance for diagnosis. J Invertebr Pathol. 2008;99(1):92-5.
Gillard, M., Charriere, J. D., & Belloy, L. (2008). Distribution of Paenibacillus larvae spores inside honey bee colonies and its relevance for diagnosis. Journal of Invertebrate Pathology, 99(1), 92-5. https://doi.org/10.1016/j.jip.2008.05.010
Gillard M, Charriere JD, Belloy L. Distribution of Paenibacillus Larvae Spores Inside Honey Bee Colonies and Its Relevance for Diagnosis. J Invertebr Pathol. 2008;99(1):92-5. PubMed PMID: 18573258.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of Paenibacillus larvae spores inside honey bee colonies and its relevance for diagnosis. AU - Gillard,M, AU - Charriere,J D, AU - Belloy,L, Y1 - 2008/06/05/ PY - 2008/01/17/received PY - 2008/05/21/revised PY - 2008/05/25/accepted PY - 2008/6/25/pubmed PY - 2008/11/7/medline PY - 2008/6/25/entrez SP - 92 EP - 5 JF - Journal of invertebrate pathology JO - J. Invertebr. Pathol. VL - 99 IS - 1 N2 - One of the most important factors affecting the development of honey bee colonies is infectious diseases such as American foulbrood (AFB) caused by the spore forming Gram-positive bacterium Paenibacillus larvae. Colony inspections for AFB clinical symptoms are time consuming. Moreover, diseased cells in the early stages of the infection may easily be overlooked. In this study, we investigated whether it is possible to determine the sanitary status of a colony based on analyses of different materials collected from the hive. We analysed 237 bee samples and 67 honey samples originating from 71 colonies situated in 13 apiaries with clinical AFB occurrences. We tested whether a difference in spore load among bees inside the whole hive exists and which sample material related to its location inside the hive was the most appropriate for an early AFB diagnosis based on the culture method. Results indicated that diagnostics based on analysis of honey samples and bees collected at the hive entrance are of limited value as only 86% and 83%, respectively, of samples from AFB-symptomatic colonies were positive. Analysis of bee samples collected from the brood nest, honey chamber, and edge frame allowed the detection of all colonies showing AFB clinical symptoms. Microbiological analysis showed that more than one quarter of samples collected from colonies without AFB clinical symptoms were positive for P. larvae. Based on these results, we recommend investigating colonies by testing bee samples from the brood nest, edge frame or honey chamber for P. larvae spores. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/18573258/Distribution_of_Paenibacillus_larvae_spores_inside_honey_bee_colonies_and_its_relevance_for_diagnosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(08)00126-2 DB - PRIME DP - Unbound Medicine ER -