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Conditioning-strength dependent involvement of NMDA NR2B subtype receptor in the basolateral nucleus of amygdala in acquisition of auditory fear memory.
Neuropharmacology. 2008 Aug; 55(2):238-46.N

Abstract

It is known that N-methyl-D-aspartate (NMDA) receptor in the basolateral nucleus of amygdala (BLA) is essential for fear memory formation. NMDA NR2B and NR2A subtype receptors exhibit difference in electrophysiological and signaling properties. However, it is unclear whether these two subtype receptors have different roles in fear memory formation. Here, we provide evidence, using pharmacological blockade and genetic interference, that NR2B is involved in acquisition of auditory fear memory in a conditioning-strength dependent way. Pre-conditioning intra-BLA infusion of the NR2B selective antagonist ifenprodil or Ro25-6981 impaired 48-h auditory fear memory (AFM) induced by five but not one CS-US pairing protocol, while similar treatment with the NR2A antagonist NVP-AAM077 disrupted memory for both protocols. Consistently, genetic over-expression of NR2B C-terminal in the BLA, which interferes with the C-terminal mediated intracellular signaling, produced a severe deficit in 48-h AFM for five but not one CS-US pairing protocol, whereas over-expression of NR2A C-terminal impaired memory for both protocols. Furthermore, pre-conditioning infusion of ifenprodil down-regulated the elevated phosphorylation level of extracellular signal-regulated kinase (ERK) induced by five CS-US pairing protocol. Thus, the involvement of BLA NR2B in AFM acquisition depends on conditioning strength.

Authors+Show Affiliations

Institute of Neurobiology and State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18573264

Citation

Zhang, Xue-Han, et al. "Conditioning-strength Dependent Involvement of NMDA NR2B Subtype Receptor in the Basolateral Nucleus of Amygdala in Acquisition of Auditory Fear Memory." Neuropharmacology, vol. 55, no. 2, 2008, pp. 238-46.
Zhang XH, Liu F, Chen Q, et al. Conditioning-strength dependent involvement of NMDA NR2B subtype receptor in the basolateral nucleus of amygdala in acquisition of auditory fear memory. Neuropharmacology. 2008;55(2):238-46.
Zhang, X. H., Liu, F., Chen, Q., Zhang, C. L., Zhuo, M., Xiong, Z. Q., & Li, B. M. (2008). Conditioning-strength dependent involvement of NMDA NR2B subtype receptor in the basolateral nucleus of amygdala in acquisition of auditory fear memory. Neuropharmacology, 55(2), 238-46. https://doi.org/10.1016/j.neuropharm.2008.05.030
Zhang XH, et al. Conditioning-strength Dependent Involvement of NMDA NR2B Subtype Receptor in the Basolateral Nucleus of Amygdala in Acquisition of Auditory Fear Memory. Neuropharmacology. 2008;55(2):238-46. PubMed PMID: 18573264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Conditioning-strength dependent involvement of NMDA NR2B subtype receptor in the basolateral nucleus of amygdala in acquisition of auditory fear memory. AU - Zhang,Xue-Han, AU - Liu,Fang, AU - Chen,Qian, AU - Zhang,Chun-Lei, AU - Zhuo,Min, AU - Xiong,Zhi-Qi, AU - Li,Bao-Ming, Y1 - 2008/06/05/ PY - 2008/03/13/received PY - 2008/05/20/revised PY - 2008/05/20/accepted PY - 2008/6/25/pubmed PY - 2008/12/17/medline PY - 2008/6/25/entrez SP - 238 EP - 46 JF - Neuropharmacology JO - Neuropharmacology VL - 55 IS - 2 N2 - It is known that N-methyl-D-aspartate (NMDA) receptor in the basolateral nucleus of amygdala (BLA) is essential for fear memory formation. NMDA NR2B and NR2A subtype receptors exhibit difference in electrophysiological and signaling properties. However, it is unclear whether these two subtype receptors have different roles in fear memory formation. Here, we provide evidence, using pharmacological blockade and genetic interference, that NR2B is involved in acquisition of auditory fear memory in a conditioning-strength dependent way. Pre-conditioning intra-BLA infusion of the NR2B selective antagonist ifenprodil or Ro25-6981 impaired 48-h auditory fear memory (AFM) induced by five but not one CS-US pairing protocol, while similar treatment with the NR2A antagonist NVP-AAM077 disrupted memory for both protocols. Consistently, genetic over-expression of NR2B C-terminal in the BLA, which interferes with the C-terminal mediated intracellular signaling, produced a severe deficit in 48-h AFM for five but not one CS-US pairing protocol, whereas over-expression of NR2A C-terminal impaired memory for both protocols. Furthermore, pre-conditioning infusion of ifenprodil down-regulated the elevated phosphorylation level of extracellular signal-regulated kinase (ERK) induced by five CS-US pairing protocol. Thus, the involvement of BLA NR2B in AFM acquisition depends on conditioning strength. SN - 0028-3908 UR - https://www.unboundmedicine.com/medline/citation/18573264/Conditioning_strength_dependent_involvement_of_NMDA_NR2B_subtype_receptor_in_the_basolateral_nucleus_of_amygdala_in_acquisition_of_auditory_fear_memory_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(08)00161-5 DB - PRIME DP - Unbound Medicine ER -