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Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin.
J Chromatogr A. 2008 Aug 01; 1201(1):43-7.JC

Abstract

A limitation of large-scale viticultural trials is the time and cost of comprehensive compositional analysis of the fruit by high-performance liquid chromatography (HPLC). In addition, separate methods have generally been required to identify and quantify different classes of metabolites. To address these shortcomings a reversed-phase HPLC method was developed to simultaneously separate the anthocyanins and flavonols present in grape skins. The method employs a methanol and water gradient acidified with 10% formic acid with a run-time of 48 min including re-equilibration. Identity of anthocyanins and flavonols in Shiraz (Vitis vinifera L.) skin was confirmed by mass spectral analysis.

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Publisher Full Text

Authors+Show Affiliations

Downey MO
Viticulture, Future Farming Systems Research, Department of Primary Industries Victoria, P.O. Box 905, Mildura, Vic. 3502, Australia. mark.downey@dpi.vic.gov.au
Rochfort S
No affiliation info available

MeSH

Alcoholic BeveragesAnthocyaninsChromatography, High Pressure LiquidFlavonolsFruitMass SpectrometryVitis

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18573501

Citation

Downey, Mark O., and Simone Rochfort. "Simultaneous Separation By Reversed-phase High-performance Liquid Chromatography and Mass Spectral Identification of Anthocyanins and Flavonols in Shiraz Grape Skin." Journal of Chromatography. A, vol. 1201, no. 1, 2008, pp. 43-7.
Downey MO, Rochfort S. Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin. J Chromatogr A. 2008;1201(1):43-7.
Downey, M. O., & Rochfort, S. (2008). Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin. Journal of Chromatography. A, 1201(1), 43-7. https://doi.org/10.1016/j.chroma.2008.06.002
Downey MO, Rochfort S. Simultaneous Separation By Reversed-phase High-performance Liquid Chromatography and Mass Spectral Identification of Anthocyanins and Flavonols in Shiraz Grape Skin. J Chromatogr A. 2008 Aug 1;1201(1):43-7. PubMed PMID: 18573501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin. AU - Downey,Mark O, AU - Rochfort,Simone, Y1 - 2008/06/06/ PY - 2008/04/01/received PY - 2008/05/27/revised PY - 2008/06/02/accepted PY - 2008/6/25/pubmed PY - 2008/9/25/medline PY - 2008/6/25/entrez SP - 43 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1201 IS - 1 N2 - A limitation of large-scale viticultural trials is the time and cost of comprehensive compositional analysis of the fruit by high-performance liquid chromatography (HPLC). In addition, separate methods have generally been required to identify and quantify different classes of metabolites. To address these shortcomings a reversed-phase HPLC method was developed to simultaneously separate the anthocyanins and flavonols present in grape skins. The method employs a methanol and water gradient acidified with 10% formic acid with a run-time of 48 min including re-equilibration. Identity of anthocyanins and flavonols in Shiraz (Vitis vinifera L.) skin was confirmed by mass spectral analysis. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18573501/Simultaneous_separation_by_reversed_phase_high_performance_liquid_chromatography_and_mass_spectral_identification_of_anthocyanins_and_flavonols_in_Shiraz_grape_skin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)00987-4 DB - PRIME DP - Unbound Medicine ER -