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[Laboratory testing of specimens from patients with viral encephalitis from some regions of China].

Abstract

OBJECTIVE

To understand the viral etiology of viral encephalitis in China by detecting IgM antibody and viral RNA in the clinical samples of patients from some provinces of China by enzyme-linked immunosorbent assay and polymerase chain reaction.

METHOD

Serum and cerebrospinal fluid samples of 771 patients with viral encephalitis or meningitis were collected from six provinces of China and were stored at -20 degrees C or -70 degrees C. Enzyme-linked immunosorbent assays were used for detection of IgM antibody to Japanese encephalitis virus, coxsackievirus, echovirus, herpes simplex virus, measles virus, varicella-zoster virus, mumps virus, cytomegalovirus and Epstein-Barr virus. Polymerase chain reaction was applied for the detection of viral RNA of enteroviruses and seadornavirus.

RESULTS

IgM antibody was detected in 567 of 771 (73.5%) cases. The most common pathogen was Japanese encephalitis virus (47.0%, 362/771), followed by mumps virus (10.6%, 82/771), enteroviruses (8.8%, 68/771), herpes simplex virus (5.7%, 44/771), measles virus (0.4%, 3/771), varicella-zoster virus (0.4%, 3/771), Epstein-Barr virus (0.4%, 3/771), and cytomegalovirus (0.3%, 2/771). Enterovirus was positive in 8 cases, seadornavirus was negative in all the cases by PCR.

CONCLUSION

According to the study, Japanese encephalitis was the most important encephalitis in China. Mumps virus was another important pathogen. Enteroviruses and herpes simplex virus were also important pathogens.

Authors+Show Affiliations

Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18574526

Citation

Xu, Zi-qian, et al. "[Laboratory Testing of Specimens From Patients With Viral Encephalitis From some Regions of China]." Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, vol. 22, no. 2, 2008, pp. 98-100.
Xu ZQ, Fu SH, Zhang YP, et al. [Laboratory testing of specimens from patients with viral encephalitis from some regions of China]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008;22(2):98-100.
Xu, Z. Q., Fu, S. H., Zhang, Y. P., Li, X. L., Gao, X. Y., Wang, L., Cao, Y. X., Xu, L. H., Jin, Y., Tang, Q., & Liang, G. D. (2008). [Laboratory testing of specimens from patients with viral encephalitis from some regions of China]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, 22(2), 98-100.
Xu ZQ, et al. [Laboratory Testing of Specimens From Patients With Viral Encephalitis From some Regions of China]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2008;22(2):98-100. PubMed PMID: 18574526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Laboratory testing of specimens from patients with viral encephalitis from some regions of China]. AU - Xu,Zi-qian, AU - Fu,Shi-hong, AU - Zhang,Yan-ping, AU - Li,Xing-le, AU - Gao,Xiao-yan, AU - Wang,Lei, AU - Cao,Yu-xi, AU - Xu,Li-hong, AU - Jin,Yu, AU - Tang,Qing, AU - Liang,Guo-dong, PY - 2008/6/25/pubmed PY - 2008/12/23/medline PY - 2008/6/25/entrez SP - 98 EP - 100 JF - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology JO - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi VL - 22 IS - 2 N2 - OBJECTIVE: To understand the viral etiology of viral encephalitis in China by detecting IgM antibody and viral RNA in the clinical samples of patients from some provinces of China by enzyme-linked immunosorbent assay and polymerase chain reaction. METHOD: Serum and cerebrospinal fluid samples of 771 patients with viral encephalitis or meningitis were collected from six provinces of China and were stored at -20 degrees C or -70 degrees C. Enzyme-linked immunosorbent assays were used for detection of IgM antibody to Japanese encephalitis virus, coxsackievirus, echovirus, herpes simplex virus, measles virus, varicella-zoster virus, mumps virus, cytomegalovirus and Epstein-Barr virus. Polymerase chain reaction was applied for the detection of viral RNA of enteroviruses and seadornavirus. RESULTS: IgM antibody was detected in 567 of 771 (73.5%) cases. The most common pathogen was Japanese encephalitis virus (47.0%, 362/771), followed by mumps virus (10.6%, 82/771), enteroviruses (8.8%, 68/771), herpes simplex virus (5.7%, 44/771), measles virus (0.4%, 3/771), varicella-zoster virus (0.4%, 3/771), Epstein-Barr virus (0.4%, 3/771), and cytomegalovirus (0.3%, 2/771). Enterovirus was positive in 8 cases, seadornavirus was negative in all the cases by PCR. CONCLUSION: According to the study, Japanese encephalitis was the most important encephalitis in China. Mumps virus was another important pathogen. Enteroviruses and herpes simplex virus were also important pathogens. SN - 1003-9279 UR - https://www.unboundmedicine.com/medline/citation/18574526/[Laboratory_testing_of_specimens_from_patients_with_viral_encephalitis_from_some_regions_of_China]_ DB - PRIME DP - Unbound Medicine ER -