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Natural sesquiterpene lactones are active against Leishmania mexicana.
J Parasitol. 2008 Oct; 94(5):1143-9.JP

Abstract

In this paper, the effects of 3 natural sesquiterpene lactones, i.e., helenalin (Hln), mexicanin (Mxc), and dehydroleucodine (DhL), were evaluated using cultured Leishmania mexicana promastigotes. It was observed that the compounds inhibited the in vitro growth of the parasites at relatively low concentrations. The effect was rapid and irreversible with an estimated IC50 of 2-4 microM, while all the lactones were more effective than ketoconazole. Moreover, these compounds exhibited low cytotoxicity for mammalian cells. Hln induced strong vacuolization of the parasite cytoplasm, although pericellular microtubules were preserved. The 3 lactones induced DNA fragmentation as judged by the high labeling with the fluorescent TUNEL method, which was confirmed by electrophoresis on agarose gels. The ability of the parasites to invade Vero cells was also decreased by exposure to low concentrations of the compounds. We conclude that these compounds can affect the parasite's life cycle, possibly through multiple mechanisms. Identification of the molecular targets of these natural products and their effects on amastigotes should be determined to evaluate the possible therapeutic use of the compounds against leishmaniasis.

Authors+Show Affiliations

Laboratorio de Biología y Fisiología Celular Dr. Francisco Bertini, Instituto de Histología y Embriología-CONICET, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18576826

Citation

Barrera, Patricia A., et al. "Natural Sesquiterpene Lactones Are Active Against Leishmania Mexicana." The Journal of Parasitology, vol. 94, no. 5, 2008, pp. 1143-9.
Barrera PA, Jimenez-Ortiz V, Tonn C, et al. Natural sesquiterpene lactones are active against Leishmania mexicana. J Parasitol. 2008;94(5):1143-9.
Barrera, P. A., Jimenez-Ortiz, V., Tonn, C., Giordano, O., Galanti, N., & Sosa, M. A. (2008). Natural sesquiterpene lactones are active against Leishmania mexicana. The Journal of Parasitology, 94(5), 1143-9. https://doi.org/10.1645/GE-1501.1
Barrera PA, et al. Natural Sesquiterpene Lactones Are Active Against Leishmania Mexicana. J Parasitol. 2008;94(5):1143-9. PubMed PMID: 18576826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural sesquiterpene lactones are active against Leishmania mexicana. AU - Barrera,Patricia A, AU - Jimenez-Ortiz,Verónica, AU - Tonn,Carlos, AU - Giordano,Oscar, AU - Galanti,Norbel, AU - Sosa,Miguel A, PY - 2007/10/08/received PY - 2008/01/02/accepted PY - 2008/6/26/pubmed PY - 2009/1/3/medline PY - 2008/6/26/entrez SP - 1143 EP - 9 JF - The Journal of parasitology JO - J. Parasitol. VL - 94 IS - 5 N2 - In this paper, the effects of 3 natural sesquiterpene lactones, i.e., helenalin (Hln), mexicanin (Mxc), and dehydroleucodine (DhL), were evaluated using cultured Leishmania mexicana promastigotes. It was observed that the compounds inhibited the in vitro growth of the parasites at relatively low concentrations. The effect was rapid and irreversible with an estimated IC50 of 2-4 microM, while all the lactones were more effective than ketoconazole. Moreover, these compounds exhibited low cytotoxicity for mammalian cells. Hln induced strong vacuolization of the parasite cytoplasm, although pericellular microtubules were preserved. The 3 lactones induced DNA fragmentation as judged by the high labeling with the fluorescent TUNEL method, which was confirmed by electrophoresis on agarose gels. The ability of the parasites to invade Vero cells was also decreased by exposure to low concentrations of the compounds. We conclude that these compounds can affect the parasite's life cycle, possibly through multiple mechanisms. Identification of the molecular targets of these natural products and their effects on amastigotes should be determined to evaluate the possible therapeutic use of the compounds against leishmaniasis. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/18576826/Natural_sesquiterpene_lactones_are_active_against_Leishmania_mexicana_ L2 - http://www.journalofparasitology.org/doi/10.1645/GE-1501.1?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -