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Zinc oxide/redox mediator composite films-based sensor for electrochemical detection of important biomolecules.
Anal Biochem. 2008 Sep 15; 380(2):174-83.AB

Abstract

Electrochemical oxidation of serotonin (SN) onto zinc oxide (ZnO)-coated glassy carbon electrode (GCE) results in the generation of redox mediators (RMs) that are strongly adsorbed on electrode surface. The electrochemical properties of zinc oxide-electrogenerated redox mediator (ZnO/RM) (inorganic/organic) hybrid film-coated electrode has been studied using cyclic voltammetry (CV). The scanning electron microscope (SEM), atomic force microscope (AFM), and electrochemical techniques proved the immobilization of ZnO/RM core/shell microparticles on the electrode surface. The GCE modified with ZnO/RM hybrid film showed two reversible redox peaks in acidic solution, and the redox peaks were found to be pH dependent with slopes of -62 and -60 mV/pH, which are very close to the Nernst behavior. The GCE/ZnO/RM-modified electrode exhibited excellent electrocatalytic activity toward the oxidations of ascorbic acid (AA), dopamine (DA), and uric acid (UA) in 0.1M phosphate buffer solution (PBS, pH 7.0). Indeed, ZnO/RM-coated GCE separated the anodic oxidation waves of DA, AA, and UA with well-defined peak separations in their mixture solution. Consequently, the GCE/ZnO/RMs were used for simultaneous detection of DA, AA, and UA in their mixture solution. Using CV, calibration curves for DA, AA, and UA were obtained over the range of 6.0 x 10(-6) to 9.6 x 10(-4)M, 1.5 x 10(-5) to 2.4 x 10(-4)M, and 5.0 x 10(-5) to 8 x 10(-4)M with correlation coefficients of 0.992, 0.991, and 0.989, respectively. Moreover, ZnO/RM-modified GCE had good stability and antifouling properties.

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Authors+Show Affiliations

Tang CF
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, sec 3 Chung-Hsiao East Road, Taipei 106, Taiwan, Republic of China.
Kumar SA
No affiliation info available
Chen SM
No affiliation info available

MeSH

Ascorbic AcidBiosensing TechniquesCarbonDopamineElectrochemistryElectrodesHydrogen-Ion ConcentrationMicroscopy, Atomic ForceMicroscopy, Electron, ScanningOxidation-ReductionSerotoninUric AcidZinc Oxide

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18577367

Citation

Tang, Chun-Fang, et al. "Zinc Oxide/redox Mediator Composite Films-based Sensor for Electrochemical Detection of Important Biomolecules." Analytical Biochemistry, vol. 380, no. 2, 2008, pp. 174-83.
Tang CF, Kumar SA, Chen SM. Zinc oxide/redox mediator composite films-based sensor for electrochemical detection of important biomolecules. Anal Biochem. 2008;380(2):174-83.
Tang, C. F., Kumar, S. A., & Chen, S. M. (2008). Zinc oxide/redox mediator composite films-based sensor for electrochemical detection of important biomolecules. Analytical Biochemistry, 380(2), 174-83. https://doi.org/10.1016/j.ab.2008.06.004
Tang CF, Kumar SA, Chen SM. Zinc Oxide/redox Mediator Composite Films-based Sensor for Electrochemical Detection of Important Biomolecules. Anal Biochem. 2008 Sep 15;380(2):174-83. PubMed PMID: 18577367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Zinc oxide/redox mediator composite films-based sensor for electrochemical detection of important biomolecules. AU - Tang,Chun-Fang, AU - Kumar,S Ashok, AU - Chen,Shen-Ming, Y1 - 2008/06/07/ PY - 2008/01/02/received PY - 2008/05/19/revised PY - 2008/06/01/accepted PY - 2008/6/26/pubmed PY - 2008/9/24/medline PY - 2008/6/26/entrez SP - 174 EP - 83 JF - Analytical biochemistry JO - Anal Biochem VL - 380 IS - 2 N2 - Electrochemical oxidation of serotonin (SN) onto zinc oxide (ZnO)-coated glassy carbon electrode (GCE) results in the generation of redox mediators (RMs) that are strongly adsorbed on electrode surface. The electrochemical properties of zinc oxide-electrogenerated redox mediator (ZnO/RM) (inorganic/organic) hybrid film-coated electrode has been studied using cyclic voltammetry (CV). The scanning electron microscope (SEM), atomic force microscope (AFM), and electrochemical techniques proved the immobilization of ZnO/RM core/shell microparticles on the electrode surface. The GCE modified with ZnO/RM hybrid film showed two reversible redox peaks in acidic solution, and the redox peaks were found to be pH dependent with slopes of -62 and -60 mV/pH, which are very close to the Nernst behavior. The GCE/ZnO/RM-modified electrode exhibited excellent electrocatalytic activity toward the oxidations of ascorbic acid (AA), dopamine (DA), and uric acid (UA) in 0.1M phosphate buffer solution (PBS, pH 7.0). Indeed, ZnO/RM-coated GCE separated the anodic oxidation waves of DA, AA, and UA with well-defined peak separations in their mixture solution. Consequently, the GCE/ZnO/RMs were used for simultaneous detection of DA, AA, and UA in their mixture solution. Using CV, calibration curves for DA, AA, and UA were obtained over the range of 6.0 x 10(-6) to 9.6 x 10(-4)M, 1.5 x 10(-5) to 2.4 x 10(-4)M, and 5.0 x 10(-5) to 8 x 10(-4)M with correlation coefficients of 0.992, 0.991, and 0.989, respectively. Moreover, ZnO/RM-modified GCE had good stability and antifouling properties. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/18577367/Zinc_oxide/redox_mediator_composite_films_based_sensor_for_electrochemical_detection_of_important_biomolecules_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(08)00371-0 DB - PRIME DP - Unbound Medicine ER -
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