Tags

Type your tag names separated by a space and hit enter

Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting.
Anal Biochem. 2008 Sep 15; 380(2):276-81.AB

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral fingerprints for 12 species of fungi of the genus Aspergillus and 5 different strains of Aspergillus flavus. Prior to MALDI-TOF MS analysis, the fungi were subjected to three 1-min bead beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contain abundant peaks in the range of 5 to 20kDa and may be used to discriminate between species unambiguously. A discriminant analysis using all peaks from the MALDI-TOF MS data yielded error rates for classification of 0 and 18.75% for resubstitution and cross-validation methods, respectively. If a subset of 28 significant peaks is chosen, resubstitution and cross-validation error rates are 0%. Discriminant analysis of the MALDI-TOF MS data for 5 strains of A. flavus using all peaks yielded error rates for classification of 0 and 5% for resubstitution and cross-validation methods, respectively. These data indicate that MALDI-TOF MS data may be used for unambiguous identification of members of the genus Aspergillus at both the species and strain levels.

Links

Publisher Full Text

Authors+Show Affiliations

Hettick JM
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA. jhettick@cdc.gov
Green BJ
No affiliation info available
Buskirk AD
No affiliation info available
Kashon ML
No affiliation info available
Slaven JE
No affiliation info available
Janotka E
No affiliation info available
Blachere FM
No affiliation info available
Schmechel D
No affiliation info available
Beezhold DH
No affiliation info available

MeSH

AspergillusAspergillus flavusCluster AnalysisSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18577370

Citation

Hettick, Justin M., et al. "Discrimination of Aspergillus Isolates at the Species and Strain Level By Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Fingerprinting." Analytical Biochemistry, vol. 380, no. 2, 2008, pp. 276-81.
Hettick JM, Green BJ, Buskirk AD, et al. Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting. Anal Biochem. 2008;380(2):276-81.
Hettick, J. M., Green, B. J., Buskirk, A. D., Kashon, M. L., Slaven, J. E., Janotka, E., Blachere, F. M., Schmechel, D., & Beezhold, D. H. (2008). Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting. Analytical Biochemistry, 380(2), 276-81. https://doi.org/10.1016/j.ab.2008.05.051
Hettick JM, et al. Discrimination of Aspergillus Isolates at the Species and Strain Level By Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Fingerprinting. Anal Biochem. 2008 Sep 15;380(2):276-81. PubMed PMID: 18577370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting. AU - Hettick,Justin M, AU - Green,Brett J, AU - Buskirk,Amanda D, AU - Kashon,Michael L, AU - Slaven,James E, AU - Janotka,Erika, AU - Blachere,Francoise M, AU - Schmechel,Detlef, AU - Beezhold,Donald H, Y1 - 2008/06/05/ PY - 2008/04/03/received PY - 2008/05/29/revised PY - 2008/05/30/accepted PY - 2008/6/26/pubmed PY - 2008/9/24/medline PY - 2008/6/26/entrez SP - 276 EP - 81 JF - Analytical biochemistry JO - Anal Biochem VL - 380 IS - 2 N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral fingerprints for 12 species of fungi of the genus Aspergillus and 5 different strains of Aspergillus flavus. Prior to MALDI-TOF MS analysis, the fungi were subjected to three 1-min bead beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contain abundant peaks in the range of 5 to 20kDa and may be used to discriminate between species unambiguously. A discriminant analysis using all peaks from the MALDI-TOF MS data yielded error rates for classification of 0 and 18.75% for resubstitution and cross-validation methods, respectively. If a subset of 28 significant peaks is chosen, resubstitution and cross-validation error rates are 0%. Discriminant analysis of the MALDI-TOF MS data for 5 strains of A. flavus using all peaks yielded error rates for classification of 0 and 5% for resubstitution and cross-validation methods, respectively. These data indicate that MALDI-TOF MS data may be used for unambiguous identification of members of the genus Aspergillus at both the species and strain levels. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/18577370/Discrimination_of_Aspergillus_isolates_at_the_species_and_strain_level_by_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_fingerprinting_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(08)00376-X DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓23]

Related Citations

More