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NF-kappaB inhibitors induce lytic cytotoxicity in Epstein-Barr virus-positive nasopharyngeal carcinoma cells.
Cell Biol Int. 2008 Aug; 32(8):1006-13.CB

Abstract

Epstein-Barr virus (EBV) infection in tumor cells is generally restricted to the latent forms of viral infection. Switching the latent form of viral infection into the lytic form may induce tumor cell death. An important nuclear factor, nuclear factor (NF)-kappaB, is thought to play an essential role in EBV lytic infection; high levels of NF-kappaB can inhibit EBV lytic replication. In this study, we tested the effect of inducing EBV lytic replication using two NF-kappaB inhibitors: Bay11-7082 and Z-LLF-CHO, to reveal the possibility of targeting EBV-positive cancer therapy with these two NF-kappaB inhibitors. Our results showed that Bay11-7082 and Z-LLF-CHO reactivated EBV in a dose-dependent manner, thus resulting in EBV-positive 5-8F cell death. In contrast, there was no significant effect on EBV-negative HNE3 cells. When ganciclovir was used in combination with either Bay11-7082 or Z-LLF-CHO to treat 5-8F cells, the cytotoxic effect of the NF-kappaB inhibitor was amplified. The finding indicates that inhibiting the NF-kappaB activity of EBV-positive cells can induce lytic replication of EBV and cause lytic cytotoxicity against these cells.

Authors+Show Affiliations

Cancer Research Institute, Xiangya School of Medicine, Central South University, and Medicine Experimental Center, The Third Xiangya Hospital, Changsha, 410078 Hunan, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18579417

Citation

Liu, Su-Fang, et al. "NF-kappaB Inhibitors Induce Lytic Cytotoxicity in Epstein-Barr Virus-positive Nasopharyngeal Carcinoma Cells." Cell Biology International, vol. 32, no. 8, 2008, pp. 1006-13.
Liu SF, Wang H, Lin XC, et al. NF-kappaB inhibitors induce lytic cytotoxicity in Epstein-Barr virus-positive nasopharyngeal carcinoma cells. Cell Biol Int. 2008;32(8):1006-13.
Liu, S. F., Wang, H., Lin, X. C., Xiang, H., Deng, X. Y., Li, W., Tang, M., & Cao, Y. (2008). NF-kappaB inhibitors induce lytic cytotoxicity in Epstein-Barr virus-positive nasopharyngeal carcinoma cells. Cell Biology International, 32(8), 1006-13. https://doi.org/10.1016/j.cellbi.2008.03.022
Liu SF, et al. NF-kappaB Inhibitors Induce Lytic Cytotoxicity in Epstein-Barr Virus-positive Nasopharyngeal Carcinoma Cells. Cell Biol Int. 2008;32(8):1006-13. PubMed PMID: 18579417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NF-kappaB inhibitors induce lytic cytotoxicity in Epstein-Barr virus-positive nasopharyngeal carcinoma cells. AU - Liu,Su-Fang, AU - Wang,Hai, AU - Lin,Xu-Chi, AU - Xiang,Hui, AU - Deng,Xi-Yun, AU - Li,Wei, AU - Tang,Min, AU - Cao,Ya, Y1 - 2008/04/08/ PY - 2007/11/27/received PY - 2008/02/08/revised PY - 2008/03/28/accepted PY - 2008/6/27/pubmed PY - 2008/10/15/medline PY - 2008/6/27/entrez SP - 1006 EP - 13 JF - Cell biology international JO - Cell Biol Int VL - 32 IS - 8 N2 - Epstein-Barr virus (EBV) infection in tumor cells is generally restricted to the latent forms of viral infection. Switching the latent form of viral infection into the lytic form may induce tumor cell death. An important nuclear factor, nuclear factor (NF)-kappaB, is thought to play an essential role in EBV lytic infection; high levels of NF-kappaB can inhibit EBV lytic replication. In this study, we tested the effect of inducing EBV lytic replication using two NF-kappaB inhibitors: Bay11-7082 and Z-LLF-CHO, to reveal the possibility of targeting EBV-positive cancer therapy with these two NF-kappaB inhibitors. Our results showed that Bay11-7082 and Z-LLF-CHO reactivated EBV in a dose-dependent manner, thus resulting in EBV-positive 5-8F cell death. In contrast, there was no significant effect on EBV-negative HNE3 cells. When ganciclovir was used in combination with either Bay11-7082 or Z-LLF-CHO to treat 5-8F cells, the cytotoxic effect of the NF-kappaB inhibitor was amplified. The finding indicates that inhibiting the NF-kappaB activity of EBV-positive cells can induce lytic replication of EBV and cause lytic cytotoxicity against these cells. SN - 1065-6995 UR - https://www.unboundmedicine.com/medline/citation/18579417/NF_kappaB_inhibitors_induce_lytic_cytotoxicity_in_Epstein_Barr_virus_positive_nasopharyngeal_carcinoma_cells_ L2 - https://doi.org/10.1016/j.cellbi.2008.03.022 DB - PRIME DP - Unbound Medicine ER -