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Presence of a large amount of herpes simplex virus genome in tear fluid of herpetic stromal keratitis and persistent epithelial defect patients.
Semin Ophthalmol 2008 Jul-Aug; 23(4):217-20SO

Abstract

Herpetic eye diseases exhibit various clinical manifestations making a diagnosis difficult in some patients. We quantitated herpes simplex virus (HSV) genomes in the tear fluid and aqueous humor obtained from patients with various herpetic eye diseases by real time PCR. The resulting amounts of HSV-DNA in herpetic epithelial keratitis (HEK), herpetic stromal keratitis (HSK) in active phase, and persistent epithelial defects (PED) were 3.9 x 10(6) copies (detection rate, 81.1%), 8.9 x 10(5) copies (detection rate, 59.1%), and 9.2 x 10(4) copies (detection rate, 88.9%), respectively. In the tear samples obtained from quiescent phase of HSK and endotheliitis, no HSV-DNA was detected. In the aqueous humor of uveitis patients, HSV-DNA was found 3.8 x 10(5) copies/ml (detection rate, 16.7%). Previous studies have shown that active viral replication is not directly related to the persistent epithelial defects and progressive HSK. A relatively high level of HSV-DNA, however, was detected in the tear samples of these two disease forms, although the source of the viral replication was not identified. These findings might bring new ideas about the mechanisms of developments in HSK and PED.

Authors+Show Affiliations

Department of Ophthalmology, Kinki University School of Medicine, Osaka, Japan. fukuda@ganka.med.kindai.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18584558

Citation

Fukuda, Masahiko, et al. "Presence of a Large Amount of Herpes Simplex Virus Genome in Tear Fluid of Herpetic Stromal Keratitis and Persistent Epithelial Defect Patients." Seminars in Ophthalmology, vol. 23, no. 4, 2008, pp. 217-20.
Fukuda M, Deai T, Higaki S, et al. Presence of a large amount of herpes simplex virus genome in tear fluid of herpetic stromal keratitis and persistent epithelial defect patients. Semin Ophthalmol. 2008;23(4):217-20.
Fukuda, M., Deai, T., Higaki, S., Hayashi, K., & Shimomura, Y. (2008). Presence of a large amount of herpes simplex virus genome in tear fluid of herpetic stromal keratitis and persistent epithelial defect patients. Seminars in Ophthalmology, 23(4), pp. 217-20. doi:10.1080/08820530802111366.
Fukuda M, et al. Presence of a Large Amount of Herpes Simplex Virus Genome in Tear Fluid of Herpetic Stromal Keratitis and Persistent Epithelial Defect Patients. Semin Ophthalmol. 2008;23(4):217-20. PubMed PMID: 18584558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presence of a large amount of herpes simplex virus genome in tear fluid of herpetic stromal keratitis and persistent epithelial defect patients. AU - Fukuda,Masahiko, AU - Deai,Tatsunori, AU - Higaki,Shiro, AU - Hayashi,Kozaburo, AU - Shimomura,Yoshikazu, PY - 2008/6/28/pubmed PY - 2008/9/5/medline PY - 2008/6/28/entrez SP - 217 EP - 20 JF - Seminars in ophthalmology JO - Semin Ophthalmol VL - 23 IS - 4 N2 - Herpetic eye diseases exhibit various clinical manifestations making a diagnosis difficult in some patients. We quantitated herpes simplex virus (HSV) genomes in the tear fluid and aqueous humor obtained from patients with various herpetic eye diseases by real time PCR. The resulting amounts of HSV-DNA in herpetic epithelial keratitis (HEK), herpetic stromal keratitis (HSK) in active phase, and persistent epithelial defects (PED) were 3.9 x 10(6) copies (detection rate, 81.1%), 8.9 x 10(5) copies (detection rate, 59.1%), and 9.2 x 10(4) copies (detection rate, 88.9%), respectively. In the tear samples obtained from quiescent phase of HSK and endotheliitis, no HSV-DNA was detected. In the aqueous humor of uveitis patients, HSV-DNA was found 3.8 x 10(5) copies/ml (detection rate, 16.7%). Previous studies have shown that active viral replication is not directly related to the persistent epithelial defects and progressive HSK. A relatively high level of HSV-DNA, however, was detected in the tear samples of these two disease forms, although the source of the viral replication was not identified. These findings might bring new ideas about the mechanisms of developments in HSK and PED. SN - 0882-0538 UR - https://www.unboundmedicine.com/medline/citation/18584558/Presence_of_a_large_amount_of_herpes_simplex_virus_genome_in_tear_fluid_of_herpetic_stromal_keratitis_and_persistent_epithelial_defect_patients_ L2 - http://www.tandfonline.com/doi/full/10.1080/08820530802111366 DB - PRIME DP - Unbound Medicine ER -