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A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis.
Talanta. 2008 Jul 15; 76(2):369-72.T

Abstract

A light-emitting diode-induced fluorescence detector (LED-FD) for capillary electrophoresis was constructed and evaluated. A lamp LED with an enhanced emission spectrum and a band pass filter was used as the excitation light source. Refractive index matching fluid (RIMF) was used in the detection cell to reduce scattering light and the noise level. The limit of detection (LOD) for fluorescein was 1.5 nM (SNR=3). The system exhibited linear responses in the range of 1 x 10(-8) to 5 x 10(-6)M (R=0.999). Application of the lamp LED-FD for the analysis of FITC-labeled ephedra herb extract by capillary electrophoresis was demonstrated.

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Authors+Show Affiliations

Xu J
Department of Analytical Chemistry and Micro-Instrumentation, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Xiong Y
No affiliation info available
Chen S
No affiliation info available
Guan Y
No affiliation info available

MeSH

Electrophoresis, CapillaryFluoresceinFluorescein-5-isothiocyanateFluorescence

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18585292

Citation

Xu, Jing, et al. "A Lamp Light-emitting Diode-induced Fluorescence Detector for Capillary Electrophoresis." Talanta, vol. 76, no. 2, 2008, pp. 369-72.
Xu J, Xiong Y, Chen S, et al. A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis. Talanta. 2008;76(2):369-72.
Xu, J., Xiong, Y., Chen, S., & Guan, Y. (2008). A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis. Talanta, 76(2), 369-72. https://doi.org/10.1016/j.talanta.2008.03.027
Xu J, et al. A Lamp Light-emitting Diode-induced Fluorescence Detector for Capillary Electrophoresis. Talanta. 2008 Jul 15;76(2):369-72. PubMed PMID: 18585292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A lamp light-emitting diode-induced fluorescence detector for capillary electrophoresis. AU - Xu,Jing, AU - Xiong,Yan, AU - Chen,Shiheng, AU - Guan,Yafeng, Y1 - 2008/03/26/ PY - 2007/12/19/received PY - 2008/03/10/revised PY - 2008/03/11/accepted PY - 2008/7/1/pubmed PY - 2008/8/14/medline PY - 2008/7/1/entrez SP - 369 EP - 72 JF - Talanta JO - Talanta VL - 76 IS - 2 N2 - A light-emitting diode-induced fluorescence detector (LED-FD) for capillary electrophoresis was constructed and evaluated. A lamp LED with an enhanced emission spectrum and a band pass filter was used as the excitation light source. Refractive index matching fluid (RIMF) was used in the detection cell to reduce scattering light and the noise level. The limit of detection (LOD) for fluorescein was 1.5 nM (SNR=3). The system exhibited linear responses in the range of 1 x 10(-8) to 5 x 10(-6)M (R=0.999). Application of the lamp LED-FD for the analysis of FITC-labeled ephedra herb extract by capillary electrophoresis was demonstrated. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18585292/A_lamp_light_emitting_diode_induced_fluorescence_detector_for_capillary_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(08)00188-4 DB - PRIME DP - Unbound Medicine ER -