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Porin biogenesis activates the sigma(E) response in Salmonella hfq mutants.
Biochimie. 2008 Oct; 90(10):1539-44.B

Abstract

In Salmonella enterica, loss of RNA chaperon Hfq promotes proteolytic cleavage of anti-sigma(E) factor RseA leading to the constitutive induction of the sigma(E)-dependent envelope stress response. Seeking to identify the source of the inducing signal, in the present study we measured RseA cleavage and sigma(E)-dependent transcription in strains lacking relevant outer membrane protein (omp) genes. We found removal of the main Salmonella porin, OmpD, to markedly reduce sigma(E) activation in hfq mutant cells. Subsequent removal of LamB and of OmpC further attenuated the response, indicating that different OMPs collectively contribute to the sigma(E)-activated phenotype. Thus, loss of Hfq-mediated regulation might cause unfolded OMPs to accumulate in the periplasm, triggering the sigma(E) response. These findings corroborate the role of Hfq protein as a pleiotropic regulator of OMP biogenesis in Gram-negative bacteria.

Authors+Show Affiliations

Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18585433

Citation

Bossi, Lionello, et al. "Porin Biogenesis Activates the sigma(E) Response in Salmonella Hfq Mutants." Biochimie, vol. 90, no. 10, 2008, pp. 1539-44.
Bossi L, Maloriol D, Figueroa-Bossi N. Porin biogenesis activates the sigma(E) response in Salmonella hfq mutants. Biochimie. 2008;90(10):1539-44.
Bossi, L., Maloriol, D., & Figueroa-Bossi, N. (2008). Porin biogenesis activates the sigma(E) response in Salmonella hfq mutants. Biochimie, 90(10), 1539-44. https://doi.org/10.1016/j.biochi.2008.06.001
Bossi L, Maloriol D, Figueroa-Bossi N. Porin Biogenesis Activates the sigma(E) Response in Salmonella Hfq Mutants. Biochimie. 2008;90(10):1539-44. PubMed PMID: 18585433.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Porin biogenesis activates the sigma(E) response in Salmonella hfq mutants. AU - Bossi,Lionello, AU - Maloriol,Danièla, AU - Figueroa-Bossi,Nara, Y1 - 2008/06/10/ PY - 2008/01/26/received PY - 2008/06/04/accepted PY - 2008/7/1/pubmed PY - 2008/12/17/medline PY - 2008/7/1/entrez SP - 1539 EP - 44 JF - Biochimie JO - Biochimie VL - 90 IS - 10 N2 - In Salmonella enterica, loss of RNA chaperon Hfq promotes proteolytic cleavage of anti-sigma(E) factor RseA leading to the constitutive induction of the sigma(E)-dependent envelope stress response. Seeking to identify the source of the inducing signal, in the present study we measured RseA cleavage and sigma(E)-dependent transcription in strains lacking relevant outer membrane protein (omp) genes. We found removal of the main Salmonella porin, OmpD, to markedly reduce sigma(E) activation in hfq mutant cells. Subsequent removal of LamB and of OmpC further attenuated the response, indicating that different OMPs collectively contribute to the sigma(E)-activated phenotype. Thus, loss of Hfq-mediated regulation might cause unfolded OMPs to accumulate in the periplasm, triggering the sigma(E) response. These findings corroborate the role of Hfq protein as a pleiotropic regulator of OMP biogenesis in Gram-negative bacteria. SN - 0300-9084 UR - https://www.unboundmedicine.com/medline/citation/18585433/Porin_biogenesis_activates_the_sigma_E__response_in_Salmonella_hfq_mutants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-9084(08)00191-0 DB - PRIME DP - Unbound Medicine ER -