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Fluorescence imaging using a fluorescent protein with a large Stokes shift.
Methods. 2008 Jul; 45(3):223-6.M

Abstract

Keima is a far-red fluorescent protein endowed with a large Stokes shift. It absorbs light maximally at around 440nm and emits maximally at around 620nm. While the original Keima is obligately tetrameric (tKeima), the dimeric and monomeric versions (mKeima and dKeima, respectively) have been generated. More recently, a tandem dimer of Keima (tdKeima) has been developed as the brightest version. Here we describe examples, which show the usefulness of Keima for dual-color fluorescence imaging technologies, such as fluorescence cross-correlation spectroscopy (FCCS) and two-photon laser scanning microscopy (TPLSM). Keima can be used in conjunction with existing fluorescent proteins in which the Stokes shift is much smaller, with the idea that while two fluorescent proteins are excited by a single laser each will fluoresce a different color.

Authors+Show Affiliations

Laboratory for Cell Function and Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18586106

Citation

Kogure, Takako, et al. "Fluorescence Imaging Using a Fluorescent Protein With a Large Stokes Shift." Methods (San Diego, Calif.), vol. 45, no. 3, 2008, pp. 223-6.
Kogure T, Kawano H, Abe Y, et al. Fluorescence imaging using a fluorescent protein with a large Stokes shift. Methods. 2008;45(3):223-6.
Kogure, T., Kawano, H., Abe, Y., & Miyawaki, A. (2008). Fluorescence imaging using a fluorescent protein with a large Stokes shift. Methods (San Diego, Calif.), 45(3), 223-6. https://doi.org/10.1016/j.ymeth.2008.06.009
Kogure T, et al. Fluorescence Imaging Using a Fluorescent Protein With a Large Stokes Shift. Methods. 2008;45(3):223-6. PubMed PMID: 18586106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluorescence imaging using a fluorescent protein with a large Stokes shift. AU - Kogure,Takako, AU - Kawano,Hiroyuki, AU - Abe,Yukiko, AU - Miyawaki,Atsushi, Y1 - 2008/06/27/ PY - 2008/06/05/received PY - 2008/06/16/accepted PY - 2008/7/1/pubmed PY - 2008/11/19/medline PY - 2008/7/1/entrez SP - 223 EP - 6 JF - Methods (San Diego, Calif.) JO - Methods VL - 45 IS - 3 N2 - Keima is a far-red fluorescent protein endowed with a large Stokes shift. It absorbs light maximally at around 440nm and emits maximally at around 620nm. While the original Keima is obligately tetrameric (tKeima), the dimeric and monomeric versions (mKeima and dKeima, respectively) have been generated. More recently, a tandem dimer of Keima (tdKeima) has been developed as the brightest version. Here we describe examples, which show the usefulness of Keima for dual-color fluorescence imaging technologies, such as fluorescence cross-correlation spectroscopy (FCCS) and two-photon laser scanning microscopy (TPLSM). Keima can be used in conjunction with existing fluorescent proteins in which the Stokes shift is much smaller, with the idea that while two fluorescent proteins are excited by a single laser each will fluoresce a different color. SN - 1095-9130 UR - https://www.unboundmedicine.com/medline/citation/18586106/Fluorescence_imaging_using_a_fluorescent_protein_with_a_large_Stokes_shift_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1046-2023(08)00106-0 DB - PRIME DP - Unbound Medicine ER -