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Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes.
Nucleic Acids Res. 2008 Aug; 36(13):4364-80.NA

Abstract

In Saccharomyces cerevisiae, synthesis of the small ribosomal subunit requires assembly of the 35S pre-rRNA into a 90S preribosomal complex. SnoRNAs, including U3 snoRNA, and many trans-acting proteins are required for the ordered assembly and function of the 90S preribosomal complex. Here, we show that the conserved protein Mrd1p binds to the pre-rRNA early during transcription and is required for compaction of the pre-18S rRNA into SSU processome particles. We have exploited the fact that an Mrd1p-GFP fusion protein is incorporated into the 90S preribosomal complex, where it acts as a partial loss-of-function mutation. When associated with the pre-rRNA, Mrd1p-GFP functionally interacts with the essential Pwp2, Mpp10 and U3 snoRNP subcomplexes that are functionally interconnected in the 90S preribosomal complex. The fusion protein can partially support 90S preribosome-mediated cleavages at the A(0)-A(2) sites. At the same time, on a substantial fraction of transcripts, the composition and/or structure of the 90S preribosomal complex is perturbed by the fusion protein in such a way that cleavage of the 35S pre-rRNA is either blocked or shifted to aberrant sites. These results show that Mrd1p is required for establishing productive structures within the 90S preribosomal complex.

Authors+Show Affiliations

Department of Molecular Biology and Functional Genomics, Stockholm University, SE-106 91, Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18586827

Citation

Segerstolpe, Asa, et al. "Mrd1p Binds to pre-rRNA Early During Transcription Independent of U3 snoRNA and Is Required for Compaction of the pre-rRNA Into Small Subunit Processomes." Nucleic Acids Research, vol. 36, no. 13, 2008, pp. 4364-80.
Segerstolpe A, Lundkvist P, Osheim YN, et al. Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes. Nucleic Acids Res. 2008;36(13):4364-80.
Segerstolpe, A., Lundkvist, P., Osheim, Y. N., Beyer, A. L., & Wieslander, L. (2008). Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes. Nucleic Acids Research, 36(13), 4364-80. https://doi.org/10.1093/nar/gkn384
Segerstolpe A, et al. Mrd1p Binds to pre-rRNA Early During Transcription Independent of U3 snoRNA and Is Required for Compaction of the pre-rRNA Into Small Subunit Processomes. Nucleic Acids Res. 2008;36(13):4364-80. PubMed PMID: 18586827.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mrd1p binds to pre-rRNA early during transcription independent of U3 snoRNA and is required for compaction of the pre-rRNA into small subunit processomes. AU - Segerstolpe,Asa, AU - Lundkvist,Pär, AU - Osheim,Yvonne N, AU - Beyer,Ann L, AU - Wieslander,Lars, Y1 - 2008/06/27/ PY - 2008/7/1/pubmed PY - 2008/8/13/medline PY - 2008/7/1/entrez SP - 4364 EP - 80 JF - Nucleic acids research JO - Nucleic Acids Res VL - 36 IS - 13 N2 - In Saccharomyces cerevisiae, synthesis of the small ribosomal subunit requires assembly of the 35S pre-rRNA into a 90S preribosomal complex. SnoRNAs, including U3 snoRNA, and many trans-acting proteins are required for the ordered assembly and function of the 90S preribosomal complex. Here, we show that the conserved protein Mrd1p binds to the pre-rRNA early during transcription and is required for compaction of the pre-18S rRNA into SSU processome particles. We have exploited the fact that an Mrd1p-GFP fusion protein is incorporated into the 90S preribosomal complex, where it acts as a partial loss-of-function mutation. When associated with the pre-rRNA, Mrd1p-GFP functionally interacts with the essential Pwp2, Mpp10 and U3 snoRNP subcomplexes that are functionally interconnected in the 90S preribosomal complex. The fusion protein can partially support 90S preribosome-mediated cleavages at the A(0)-A(2) sites. At the same time, on a substantial fraction of transcripts, the composition and/or structure of the 90S preribosomal complex is perturbed by the fusion protein in such a way that cleavage of the 35S pre-rRNA is either blocked or shifted to aberrant sites. These results show that Mrd1p is required for establishing productive structures within the 90S preribosomal complex. SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/18586827/Mrd1p_binds_to_pre_rRNA_early_during_transcription_independent_of_U3_snoRNA_and_is_required_for_compaction_of_the_pre_rRNA_into_small_subunit_processomes_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkn384 DB - PRIME DP - Unbound Medicine ER -