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Molecular and immunological characterization of novel weed pollen pan-allergens.
Allergy. 2008 Jul; 63(7):872-81.A

Abstract

BACKGROUND

Pan-allergens like profilins, calcium-binding proteins (CBPs), and nonspecific lipid transfer proteins have been suggested as possible specific markers for multiple pollen sensitizations, and could be used to predict cross-sensitization/poly-sensitization to several pollen allergens. Therefore, the purification and characterization of cross-reacting allergens in pollen is an extremely important task towards correct allergy diagnosis.

METHODS

New pan-allergens were identified by screening a ragweed pollen cDNA library with sera of patients allergic to mugwort pollen. Resulting proteins were cloned, expressed, purified and characterized.

RESULTS

We report complete cDNA sequences of two profilin isoforms (Amb a 8.01 and Amb a 8.02), two isoforms of a 2EF-hand CBP (Amb a 9.01 and Amb a 9.02), a new 3EF-hand CBP (Amb a 10) from ragweed pollen and a 2EF-hand CBP from mugwort (Art v 5). All these proteins were expressed in Escherichia coli, purified to homogeneity and characterized by biochemical and immunological means.

CONCLUSIONS

The identified proteins are novel pan-allergens and can be used as diagnostic markers for polysensitization and used in component-resolved diagnosis.

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    Authors+Show Affiliations

    Wopfner N
    Department of Molecular Biology, Christian Doppler Laboratory for Allergy Diagnosis and Therapy, University of Salzburg, Salzburg, Austria.
    Gruber P
    No affiliation info available
    Wallner M
    No affiliation info available
    Briza P
    No affiliation info available
    Ebner C
    No affiliation info available
    Mari A
    No affiliation info available
    Richter K
    No affiliation info available
    Vogel L
    No affiliation info available
    Ferreira F
    No affiliation info available

    MeSH

    AllergensAmbrosiaAmino Acid SequenceAnimalsAntigens, PlantArtemisiaBasophil Degranulation TestBasophilsCalcium-Binding ProteinsCell LineCircular DichroismCross ReactionsEF Hand MotifsElectrophoresis, Polyacrylamide GelGene LibraryHumansImmunoglobulin EMolecular Sequence DataPollenProfilinsRNA, PlantRatsRhinitis, Allergic, Seasonal

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    18588553

    Citation

    Wopfner, N, et al. "Molecular and Immunological Characterization of Novel Weed Pollen Pan-allergens." Allergy, vol. 63, no. 7, 2008, pp. 872-81.
    Wopfner N, Gruber P, Wallner M, et al. Molecular and immunological characterization of novel weed pollen pan-allergens. Allergy. 2008;63(7):872-81.
    Wopfner, N., Gruber, P., Wallner, M., Briza, P., Ebner, C., Mari, A., Richter, K., Vogel, L., & Ferreira, F. (2008). Molecular and immunological characterization of novel weed pollen pan-allergens. Allergy, 63(7), 872-81. https://doi.org/10.1111/j.1398-9995.2008.01635.x
    Wopfner N, et al. Molecular and Immunological Characterization of Novel Weed Pollen Pan-allergens. Allergy. 2008;63(7):872-81. PubMed PMID: 18588553.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Molecular and immunological characterization of novel weed pollen pan-allergens. AU - Wopfner,N, AU - Gruber,P, AU - Wallner,M, AU - Briza,P, AU - Ebner,C, AU - Mari,A, AU - Richter,K, AU - Vogel,L, AU - Ferreira,F, PY - 2008/7/1/pubmed PY - 2008/7/31/medline PY - 2008/7/1/entrez SP - 872 EP - 81 JF - Allergy JO - Allergy VL - 63 IS - 7 N2 - BACKGROUND: Pan-allergens like profilins, calcium-binding proteins (CBPs), and nonspecific lipid transfer proteins have been suggested as possible specific markers for multiple pollen sensitizations, and could be used to predict cross-sensitization/poly-sensitization to several pollen allergens. Therefore, the purification and characterization of cross-reacting allergens in pollen is an extremely important task towards correct allergy diagnosis. METHODS: New pan-allergens were identified by screening a ragweed pollen cDNA library with sera of patients allergic to mugwort pollen. Resulting proteins were cloned, expressed, purified and characterized. RESULTS: We report complete cDNA sequences of two profilin isoforms (Amb a 8.01 and Amb a 8.02), two isoforms of a 2EF-hand CBP (Amb a 9.01 and Amb a 9.02), a new 3EF-hand CBP (Amb a 10) from ragweed pollen and a 2EF-hand CBP from mugwort (Art v 5). All these proteins were expressed in Escherichia coli, purified to homogeneity and characterized by biochemical and immunological means. CONCLUSIONS: The identified proteins are novel pan-allergens and can be used as diagnostic markers for polysensitization and used in component-resolved diagnosis. SN - 1398-9995 UR - https://www.unboundmedicine.com/medline/citation/18588553/Molecular_and_immunological_characterization_of_novel_weed_pollen_pan_allergens_ L2 - https://doi.org/10.1111/j.1398-9995.2008.01635.x DB - PRIME DP - Unbound Medicine ER -