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Gastroprotective mechanisms of centipedic acid, a natural diterpene from Egletes viscosa LESS.
Biol Pharm Bull. 2008 Jul; 31(7):1351-5.BP

Abstract

This study was aimed to clarify the mechanisms of gastroprotection by centipedic acid (CPA), a natural diterpene from Egletes viscosa LESS. (Asteraceae) using ethanol-induced gastric mucosal damage in mice and gastric secretion in 4-h pylorus-ligated rats as model systems. In mice, intragastrically administered CPA (25, 50, 100 mg/kg) greatly reduced the mucosal lesions induced by 96% ethanol (0.2 ml, p.o.) by 18, 53, and 79%, respectively, whereas N-acetylcysteine (NAC, 300 mg/kg, i.p.), the reference compound produced a 50% inhibition. In 4-h pylorus-ligated rats, CPA (50 mg/kg) applied intraduodenally decreased both gastric secretory volume and total acidity. Similar to NAC, the plant diterpene effectively prevented the ethanol associated decrease in non-proteic sulfhydryls (NP-SH) and the elevated thiobarbituric acid-reactive substances (TBARS) in gastric tissue, suggesting that these compounds exert an antioxidant effect. Pretreatment of mice with indomethacin, the cyclooxygenase inhibitor but not with capsazepine, the transient receptor potential vanilloid-1 (TRPV1)-receptor antagonist greatly suppressed the gastroprotective effect of CPA. Furthermore, CPA gastroprotection was significantly attenuated in mice pretreated with L-NAME or glibenclamide the respective inhibitors of nitric oxide synthase and K(+)(ATP) channel activation. These data suggest that CPA affords gastroprotection by different and complementary mechanisms, which include a sparing effect on NP-SH reserve, and roles for endogenous prostaglandins, nitric oxide, and TRPV1-receptor and K(+)(ATP) channel activation.

Authors+Show Affiliations

Department of Physiology and Pharmacology, Post-Graduate Programme in Medical Sciences, Faculty of Medicine, Federal University of Ceará, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18591773

Citation

Guedes, Marjorie Moreira, et al. "Gastroprotective Mechanisms of Centipedic Acid, a Natural Diterpene From Egletes Viscosa LESS." Biological & Pharmaceutical Bulletin, vol. 31, no. 7, 2008, pp. 1351-5.
Guedes MM, Carvalho AC, Lima AF, et al. Gastroprotective mechanisms of centipedic acid, a natural diterpene from Egletes viscosa LESS. Biol Pharm Bull. 2008;31(7):1351-5.
Guedes, M. M., Carvalho, A. C., Lima, A. F., Lira, S. R., de Queiroz, S. S., Silveira, E. R., Santos, F. A., & Rao, V. S. (2008). Gastroprotective mechanisms of centipedic acid, a natural diterpene from Egletes viscosa LESS. Biological & Pharmaceutical Bulletin, 31(7), 1351-5.
Guedes MM, et al. Gastroprotective Mechanisms of Centipedic Acid, a Natural Diterpene From Egletes Viscosa LESS. Biol Pharm Bull. 2008;31(7):1351-5. PubMed PMID: 18591773.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gastroprotective mechanisms of centipedic acid, a natural diterpene from Egletes viscosa LESS. AU - Guedes,Marjorie Moreira, AU - Carvalho,Ana Carla da Silva, AU - Lima,Alana Fontales, AU - Lira,Silveria Regina de Sousa, AU - de Queiroz,Samia Sousa, AU - Silveira,Edilberto Rocha, AU - Santos,Flávia Almeida, AU - Rao,Vietla Satyanarayana, PY - 2008/7/2/pubmed PY - 2008/9/3/medline PY - 2008/7/2/entrez SP - 1351 EP - 5 JF - Biological & pharmaceutical bulletin JO - Biol Pharm Bull VL - 31 IS - 7 N2 - This study was aimed to clarify the mechanisms of gastroprotection by centipedic acid (CPA), a natural diterpene from Egletes viscosa LESS. (Asteraceae) using ethanol-induced gastric mucosal damage in mice and gastric secretion in 4-h pylorus-ligated rats as model systems. In mice, intragastrically administered CPA (25, 50, 100 mg/kg) greatly reduced the mucosal lesions induced by 96% ethanol (0.2 ml, p.o.) by 18, 53, and 79%, respectively, whereas N-acetylcysteine (NAC, 300 mg/kg, i.p.), the reference compound produced a 50% inhibition. In 4-h pylorus-ligated rats, CPA (50 mg/kg) applied intraduodenally decreased both gastric secretory volume and total acidity. Similar to NAC, the plant diterpene effectively prevented the ethanol associated decrease in non-proteic sulfhydryls (NP-SH) and the elevated thiobarbituric acid-reactive substances (TBARS) in gastric tissue, suggesting that these compounds exert an antioxidant effect. Pretreatment of mice with indomethacin, the cyclooxygenase inhibitor but not with capsazepine, the transient receptor potential vanilloid-1 (TRPV1)-receptor antagonist greatly suppressed the gastroprotective effect of CPA. Furthermore, CPA gastroprotection was significantly attenuated in mice pretreated with L-NAME or glibenclamide the respective inhibitors of nitric oxide synthase and K(+)(ATP) channel activation. These data suggest that CPA affords gastroprotection by different and complementary mechanisms, which include a sparing effect on NP-SH reserve, and roles for endogenous prostaglandins, nitric oxide, and TRPV1-receptor and K(+)(ATP) channel activation. SN - 0918-6158 UR - https://www.unboundmedicine.com/medline/citation/18591773/Gastroprotective_mechanisms_of_centipedic_acid_a_natural_diterpene_from_Egletes_viscosa_LESS_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/bpb/31.1351?from=PubMed DB - PRIME DP - Unbound Medicine ER -