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Procyanidin C1 from apple extracts inhibits Fc epsilon RI-mediated mast cell activation.
Int Arch Allergy Immunol. 2008; 147(3):213-21.IA

Abstract

BACKGROUND

Polyphenol-enriched fractions, which are extracted from unripe apples (Rosaceae, Malus spp.), consisting of procyanidins (polymers of catechins) are known to have an anti-allergenic effect on patients with various allergic diseases. Although it has been reported that apple extracts inhibit histamine release from mast cells, the molecular mechanisms for this anti-allergenic effect are not well understood. To elucidate the molecular mechanisms by which apple extracts induce their anti-allergenic effects, the effects of purified apple extract components on high-affinity receptors for IgE (Fc epsilon RI)-mediated mast cell activation were investigated.

METHODS

The anti-allergic effect of oral administration of apple procyanidin extracts on passive cutaneous anaphylactic responses of BALB/c mice was assessed. We evaluated the effects of procyanidin C1 (PC1) [epicatechin-(4beta-->8)-epicatechin-(4beta-->8)-epicatechin], a component of the procyanidin fraction, on mouse bone-marrow-derived mast cell degranulation, cytokine production, protein tyrosine phosphorylation and on the generation of intracellular reactive oxygen species (ROS) of cells stimulated by Fc epsilon RI cross-linking in vitro.

RESULTS

In an in vivo study, oral administration of the procyanidin fraction suppressed the mast-cell-dependent allergic reaction. In in vitro studies, PC1 dose-dependently decreased Fc epsilon RI-mediated degranulation and cytokine production of mast cells. Furthermore, PC1 inhibited tyrosine phosphorylation of Syk and linker for activation of T cells, and the ROS generation in stimulated mast cells.

CONCLUSIONS

PC1 suppresses Fc epsilon RI-mediated mast cell activation by inhibiting intracellular signaling pathways. These observations provide evidence for the anti-allergenic effects of the procyanidin-enriched apple extract.

Authors+Show Affiliations

Atopy Allergy Research Center, Juntendo University School of Medicine, Tokyo, Japan. nbnakano@med.juntendo.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18594151

Citation

Nakano, Nobuhiro, et al. "Procyanidin C1 From Apple Extracts Inhibits Fc Epsilon RI-mediated Mast Cell Activation." International Archives of Allergy and Immunology, vol. 147, no. 3, 2008, pp. 213-21.
Nakano N, Nishiyama C, Tokura T, et al. Procyanidin C1 from apple extracts inhibits Fc epsilon RI-mediated mast cell activation. Int Arch Allergy Immunol. 2008;147(3):213-21.
Nakano, N., Nishiyama, C., Tokura, T., Nagasako-Akazome, Y., Ohtake, Y., Okumura, K., & Ogawa, H. (2008). Procyanidin C1 from apple extracts inhibits Fc epsilon RI-mediated mast cell activation. International Archives of Allergy and Immunology, 147(3), 213-21. https://doi.org/10.1159/000142044
Nakano N, et al. Procyanidin C1 From Apple Extracts Inhibits Fc Epsilon RI-mediated Mast Cell Activation. Int Arch Allergy Immunol. 2008;147(3):213-21. PubMed PMID: 18594151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Procyanidin C1 from apple extracts inhibits Fc epsilon RI-mediated mast cell activation. AU - Nakano,Nobuhiro, AU - Nishiyama,Chiharu, AU - Tokura,Tomoko, AU - Nagasako-Akazome,Yoko, AU - Ohtake,Yasuyuki, AU - Okumura,Ko, AU - Ogawa,Hideoki, Y1 - 2008/07/02/ PY - 2007/09/27/received PY - 2008/03/03/accepted PY - 2008/7/3/pubmed PY - 2008/11/11/medline PY - 2008/7/3/entrez SP - 213 EP - 21 JF - International archives of allergy and immunology JO - Int Arch Allergy Immunol VL - 147 IS - 3 N2 - BACKGROUND: Polyphenol-enriched fractions, which are extracted from unripe apples (Rosaceae, Malus spp.), consisting of procyanidins (polymers of catechins) are known to have an anti-allergenic effect on patients with various allergic diseases. Although it has been reported that apple extracts inhibit histamine release from mast cells, the molecular mechanisms for this anti-allergenic effect are not well understood. To elucidate the molecular mechanisms by which apple extracts induce their anti-allergenic effects, the effects of purified apple extract components on high-affinity receptors for IgE (Fc epsilon RI)-mediated mast cell activation were investigated. METHODS: The anti-allergic effect of oral administration of apple procyanidin extracts on passive cutaneous anaphylactic responses of BALB/c mice was assessed. We evaluated the effects of procyanidin C1 (PC1) [epicatechin-(4beta-->8)-epicatechin-(4beta-->8)-epicatechin], a component of the procyanidin fraction, on mouse bone-marrow-derived mast cell degranulation, cytokine production, protein tyrosine phosphorylation and on the generation of intracellular reactive oxygen species (ROS) of cells stimulated by Fc epsilon RI cross-linking in vitro. RESULTS: In an in vivo study, oral administration of the procyanidin fraction suppressed the mast-cell-dependent allergic reaction. In in vitro studies, PC1 dose-dependently decreased Fc epsilon RI-mediated degranulation and cytokine production of mast cells. Furthermore, PC1 inhibited tyrosine phosphorylation of Syk and linker for activation of T cells, and the ROS generation in stimulated mast cells. CONCLUSIONS: PC1 suppresses Fc epsilon RI-mediated mast cell activation by inhibiting intracellular signaling pathways. These observations provide evidence for the anti-allergenic effects of the procyanidin-enriched apple extract. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/18594151/Procyanidin_C1_from_apple_extracts_inhibits_Fc_epsilon_RI_mediated_mast_cell_activation_ L2 - https://www.karger.com?DOI=10.1159/000142044 DB - PRIME DP - Unbound Medicine ER -