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Analysis of eight free progestogens in eggs by matrix solid-phase dispersion extraction and very high pressure liquid chromatography with tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jul 15; 870(2):241-6.JC

Abstract

A rapid method to identify and quantify unconjugated progestogens in eggs is presented. Samples were prepared based on matrix solid-phase dispersion (MSPD) using C18 as dispersant, followed by a clean-up step with graphitized carbon black (GCB) solid-phase extraction (SPE) cartridges. The analytes were separated by very high pressure LC on a BEH C18 column for a period of 5 min. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) was operated in the positive time-scheduled multi-reaction monitoring mode. Recovery studies were performed at two fortification levels. Average recoveries of the target compounds varied from 83.8% to 111.2% and relative standard deviations ranged from 10.5% to 23.7%. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 0.2-2.0 microg kg(-1) and 0.6-5.0 microg kg(-1), respectively. Investigation of real samples indicated that the range of progesterone in eggs was 9.9-40.0 microg kg(-1).

Authors+Show Affiliations

Central Lab, Beijing Center for Disease Control and Prevention, Beijing 100013, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18599367

Citation

Yang, Yi, et al. "Analysis of Eight Free Progestogens in Eggs By Matrix Solid-phase Dispersion Extraction and Very High Pressure Liquid Chromatography With Tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 870, no. 2, 2008, pp. 241-6.
Yang Y, Shao B, Zhang J, et al. Analysis of eight free progestogens in eggs by matrix solid-phase dispersion extraction and very high pressure liquid chromatography with tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;870(2):241-6.
Yang, Y., Shao, B., Zhang, J., Wu, Y., & Ying, J. (2008). Analysis of eight free progestogens in eggs by matrix solid-phase dispersion extraction and very high pressure liquid chromatography with tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 870(2), 241-6. https://doi.org/10.1016/j.jchromb.2008.06.021
Yang Y, et al. Analysis of Eight Free Progestogens in Eggs By Matrix Solid-phase Dispersion Extraction and Very High Pressure Liquid Chromatography With Tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jul 15;870(2):241-6. PubMed PMID: 18599367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of eight free progestogens in eggs by matrix solid-phase dispersion extraction and very high pressure liquid chromatography with tandem mass spectrometry. AU - Yang,Yi, AU - Shao,Bing, AU - Zhang,Jing, AU - Wu,Yongning, AU - Ying,Jie, Y1 - 2008/06/20/ PY - 2008/01/10/received PY - 2008/06/12/revised PY - 2008/06/17/accepted PY - 2008/7/5/pubmed PY - 2008/10/23/medline PY - 2008/7/5/entrez SP - 241 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 870 IS - 2 N2 - A rapid method to identify and quantify unconjugated progestogens in eggs is presented. Samples were prepared based on matrix solid-phase dispersion (MSPD) using C18 as dispersant, followed by a clean-up step with graphitized carbon black (GCB) solid-phase extraction (SPE) cartridges. The analytes were separated by very high pressure LC on a BEH C18 column for a period of 5 min. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) was operated in the positive time-scheduled multi-reaction monitoring mode. Recovery studies were performed at two fortification levels. Average recoveries of the target compounds varied from 83.8% to 111.2% and relative standard deviations ranged from 10.5% to 23.7%. The limits of detection (LODs) and limits of quantitation (LOQs) were in the range of 0.2-2.0 microg kg(-1) and 0.6-5.0 microg kg(-1), respectively. Investigation of real samples indicated that the range of progesterone in eggs was 9.9-40.0 microg kg(-1). SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18599367/Analysis_of_eight_free_progestogens_in_eggs_by_matrix_solid_phase_dispersion_extraction_and_very_high_pressure_liquid_chromatography_with_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00440-6 DB - PRIME DP - Unbound Medicine ER -