TY - JOUR T1 - Direct quantitative determination of amlodipine enantiomers in urine samples for pharmacokinetic study using on-line coupled isotachophoresis-capillary zone electrophoresis separation method with diode array detection. AU - Miks,Peter, AU - Maráková,Katarína, AU - Marák,Jozef, AU - Nemec,Igor, AU - Valásková,Iva a, AU - Havránek,Emil, PY - 2008/03/28/received PY - 2008/06/10/revised PY - 2008/06/17/accepted PY - 2008/7/5/pubmed PY - 2009/3/25/medline PY - 2008/7/5/entrez SP - 266 EP - 72 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 875 IS - 1 N2 - The present work illustrates possibilities of column-coupling capillary electrophoresis (CE-CE) combined with chiral selector (2-hydroxypropyl-beta-cyclodextrin, HP-beta-CD) and fiber-based diode array detection (DAD) for the direct quantitative enantioselective determination of trace drug (amlodipine, AML) in biological multicomponent ionic matrices (human urine). Capillary isotachophoresis (ITP) served as an ideal injection technique in CE-CE. Moreover, the ITP provided an effective on-line sample pretreatment prior to the capillary zone electrophoresis (CZE) separation. Enhanced separation selectivity due to the combination of different separation mechanisms (ITP vs. CZE-HP-beta-CD) enabled to obtain pure zones of the analytes, suitable for their detection and quantitation. The DAD, unlike single wavelength UV detection, enabled to characterize the purity (i.e. spectral homogeneity) of the analytes zones. A processing of the raw DAD spectra (the background correction and smoothing procedure) was essential when a trace analyte signal was evaluated. Obtained results indicated pure (i.e. spectrally homogeneous) zones of interest confirming effective ITP-CZE separation process. The proposed ITP-CZE-DAD method was characterized by favorable performance parameters (sensitivity, linearity, precision, recovery, accuracy, robustness, selectivity) and successfully applied to an enantioselective pharmacokinetic study of AML. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18599368/Direct_quantitative_determination_of_amlodipine_enantiomers_in_urine_samples_for_pharmacokinetic_study_using_on_line_coupled_isotachophoresis_capillary_zone_electrophoresis_separation_method_with_diode_array_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00446-7 DB - PRIME DP - Unbound Medicine ER -