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Autoregulated expression of the gene coding for the leucine-responsive protein, Lrp, a global regulator in Salmonella enterica serovar Typhimurium.
Microbiology (Reading). 2008 Jul; 154(Pt 7):2008-2016.M

Abstract

In this study, the lrp gene encoding the leucine-responsive regulatory protein (Lrp) in Salmonella enterica serovar Typhimurium was found to be negatively autoregulated. Its transcription start site was determined by primer extension analysis, showing that the lrp promoter is located at a different site to that inferred previously from the S. Typhimurium genome sequence. Chromosomal DNA fragments that include the promoter region were bound by purified Lrp protein in vitro, producing up to four distinct protein-DNA complexes. DNase I footprinting identified regions that were protected by the protein in vitro as well as bases that became hypersensitive to DNase I treatment following Lrp binding. A clear pattern of periodic hypersensitivity was detected between positions -130 and +15 that was consistent with wrapping of the DNA around Lrp in a nucleoprotein complex that includes the putative promoter region. Lrp-DNA interaction in this region was fully consistent with the observed repression of lrp transcription by this protein. Leucine was found to modulate Lrp-mediated autorepression by remodelling the Lrp-DNA nucleoprotein complex.

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Authors+Show Affiliations

McFarland KA
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland.
Dorman CJ
Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland.

MeSH

Bacterial ProteinsBase SequenceBinding SitesDNA FootprintingDown-RegulationGene Expression Regulation, BacterialLeucineLeucine-Responsive Regulatory ProteinMolecular Sequence DataPromoter Regions, GeneticSalmonella typhimuriumTranscription Initiation SiteTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18599829

Citation

McFarland, Kirsty A., and Charles J. Dorman. "Autoregulated Expression of the Gene Coding for the Leucine-responsive Protein, Lrp, a Global Regulator in Salmonella Enterica Serovar Typhimurium." Microbiology (Reading, England), vol. 154, no. Pt 7, 2008, pp. 2008-2016.
McFarland KA, Dorman CJ. Autoregulated expression of the gene coding for the leucine-responsive protein, Lrp, a global regulator in Salmonella enterica serovar Typhimurium. Microbiology (Reading). 2008;154(Pt 7):2008-2016.
McFarland, K. A., & Dorman, C. J. (2008). Autoregulated expression of the gene coding for the leucine-responsive protein, Lrp, a global regulator in Salmonella enterica serovar Typhimurium. Microbiology (Reading, England), 154(Pt 7), 2008-2016. https://doi.org/10.1099/mic.0.2008/018358-0
McFarland KA, Dorman CJ. Autoregulated Expression of the Gene Coding for the Leucine-responsive Protein, Lrp, a Global Regulator in Salmonella Enterica Serovar Typhimurium. Microbiology (Reading). 2008;154(Pt 7):2008-2016. PubMed PMID: 18599829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Autoregulated expression of the gene coding for the leucine-responsive protein, Lrp, a global regulator in Salmonella enterica serovar Typhimurium. AU - McFarland,Kirsty A, AU - Dorman,Charles J, PY - 2008/7/5/pubmed PY - 2008/9/18/medline PY - 2008/7/5/entrez SP - 2008 EP - 2016 JF - Microbiology (Reading, England) JO - Microbiology (Reading) VL - 154 IS - Pt 7 N2 - In this study, the lrp gene encoding the leucine-responsive regulatory protein (Lrp) in Salmonella enterica serovar Typhimurium was found to be negatively autoregulated. Its transcription start site was determined by primer extension analysis, showing that the lrp promoter is located at a different site to that inferred previously from the S. Typhimurium genome sequence. Chromosomal DNA fragments that include the promoter region were bound by purified Lrp protein in vitro, producing up to four distinct protein-DNA complexes. DNase I footprinting identified regions that were protected by the protein in vitro as well as bases that became hypersensitive to DNase I treatment following Lrp binding. A clear pattern of periodic hypersensitivity was detected between positions -130 and +15 that was consistent with wrapping of the DNA around Lrp in a nucleoprotein complex that includes the putative promoter region. Lrp-DNA interaction in this region was fully consistent with the observed repression of lrp transcription by this protein. Leucine was found to modulate Lrp-mediated autorepression by remodelling the Lrp-DNA nucleoprotein complex. SN - 1350-0872 UR - https://www.unboundmedicine.com/medline/citation/18599829/Autoregulated_expression_of_the_gene_coding_for_the_leucine_responsive_protein_Lrp_a_global_regulator_in_Salmonella_enterica_serovar_Typhimurium_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/mic.0.2008/018358-0 DB - PRIME DP - Unbound Medicine ER -