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Stemucronatoside K, a novel C(21) steroidal glycoside from Stephanotis mucronata, inhibited the cellular and humoral immune response in mice.
Int Immunopharmacol. 2008 Sep; 8(9):1231-8.II

Abstract

Stephanotis mucronata (Blanco) Merr. has been used for rheumatoid arthritis in Chinese folk herb medicine. Guided by bioactive test, a novel potent immunosuppressive C(21) steroidal glycoside stemucronatoside K (SMK) was isolated from this plant. Its structure was elucidated on the basis of the chemical evidence and extensive spectroscopic methods. We investigated the immunosuppressive effects of SMK in vitro and in vivo. SMK significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. ICR mice were immunized subcutaneously with OVA on the first day and administered intraperitoneally with SMK at the doses of 2.5, 5, and 10 mg/kg once daily for 10 days. At 24 h after the last administration, mitogen- and OVA-stimulated splenocyte proliferation, the levels of cytokines from splenocytes, and specific antibody titers in serum were measured. SMK significantly inhibited Con A-, LPS- and OVA-induced splenocyte proliferation in the immunized mice in a dose-dependent manner. OVA-specific IgG, IgG1, and IgG2b antibody titers were significantly reduced by SMK compared with the control group. SMK also significantly decreased OVA-induced interleukin-2 (IL-2), interferon-gamma (IFN-gamma), and IL-4 production from splenocytes in the OVA-immunized mice. These results demonstrated that SMK could suppress the cellular and humoral immune response in mice. This study provided evidence to understand the therapeutic effects of S. mucronata and an immunosuppressive natural product compound to further researches to be developed as immunosuppressant.

Authors+Show Affiliations

Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. yeyiping2005@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18602069

Citation

Ye, Yiping, et al. "Stemucronatoside K, a Novel C(21) Steroidal Glycoside From Stephanotis Mucronata, Inhibited the Cellular and Humoral Immune Response in Mice." International Immunopharmacology, vol. 8, no. 9, 2008, pp. 1231-8.
Ye Y, Chen F, Sun H, et al. Stemucronatoside K, a novel C(21) steroidal glycoside from Stephanotis mucronata, inhibited the cellular and humoral immune response in mice. Int Immunopharmacol. 2008;8(9):1231-8.
Ye, Y., Chen, F., Sun, H., Li, X., & Xu, S. (2008). Stemucronatoside K, a novel C(21) steroidal glycoside from Stephanotis mucronata, inhibited the cellular and humoral immune response in mice. International Immunopharmacology, 8(9), 1231-8. https://doi.org/10.1016/j.intimp.2008.04.014
Ye Y, et al. Stemucronatoside K, a Novel C(21) Steroidal Glycoside From Stephanotis Mucronata, Inhibited the Cellular and Humoral Immune Response in Mice. Int Immunopharmacol. 2008;8(9):1231-8. PubMed PMID: 18602069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stemucronatoside K, a novel C(21) steroidal glycoside from Stephanotis mucronata, inhibited the cellular and humoral immune response in mice. AU - Ye,Yiping, AU - Chen,Fengyang, AU - Sun,Hongxiang, AU - Li,Xiaoyu, AU - Xu,Shifang, Y1 - 2008/05/22/ PY - 2008/03/17/received PY - 2008/04/24/revised PY - 2008/04/28/accepted PY - 2008/7/8/pubmed PY - 2008/10/4/medline PY - 2008/7/8/entrez SP - 1231 EP - 8 JF - International immunopharmacology JO - Int Immunopharmacol VL - 8 IS - 9 N2 - Stephanotis mucronata (Blanco) Merr. has been used for rheumatoid arthritis in Chinese folk herb medicine. Guided by bioactive test, a novel potent immunosuppressive C(21) steroidal glycoside stemucronatoside K (SMK) was isolated from this plant. Its structure was elucidated on the basis of the chemical evidence and extensive spectroscopic methods. We investigated the immunosuppressive effects of SMK in vitro and in vivo. SMK significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. ICR mice were immunized subcutaneously with OVA on the first day and administered intraperitoneally with SMK at the doses of 2.5, 5, and 10 mg/kg once daily for 10 days. At 24 h after the last administration, mitogen- and OVA-stimulated splenocyte proliferation, the levels of cytokines from splenocytes, and specific antibody titers in serum were measured. SMK significantly inhibited Con A-, LPS- and OVA-induced splenocyte proliferation in the immunized mice in a dose-dependent manner. OVA-specific IgG, IgG1, and IgG2b antibody titers were significantly reduced by SMK compared with the control group. SMK also significantly decreased OVA-induced interleukin-2 (IL-2), interferon-gamma (IFN-gamma), and IL-4 production from splenocytes in the OVA-immunized mice. These results demonstrated that SMK could suppress the cellular and humoral immune response in mice. This study provided evidence to understand the therapeutic effects of S. mucronata and an immunosuppressive natural product compound to further researches to be developed as immunosuppressant. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/18602069/Stemucronatoside_K_a_novel_C_21__steroidal_glycoside_from_Stephanotis_mucronata_inhibited_the_cellular_and_humoral_immune_response_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(08)00139-2 DB - PRIME DP - Unbound Medicine ER -