Tags

Type your tag names separated by a space and hit enter

The role of syndecan-4 and attached glycosaminoglycan chains on myogenic satellite cell growth.
Matrix Biol. 2008 Sep; 27(7):619-30.MB

Abstract

The syndecans are a family of cell-surface heparan sulfate proteoglycans consisting of a core protein with covalently attached glycosaminoglycan (GAG) chains. Syndecan-4 expression in skeletal muscle is increased in growth-selected animals during proliferation. Previous studies have suggested that cell-surface heparan sulfate proteoglycans like syndecan-4 are involved in fibroblast growth factor 2 (FGF2) signaling by FGF2 binding to the heparan sulfate chains. Fibroblast growth factor 2 is a potent stimulator of muscle proliferation and an intense inhibitor of differentiation. To investigate the functional contribution of the attached syndecan-4 GAG chains, a turkey syndecan-4 full length cDNA was cloned and mutated at two or all three potential GAG attachment sites at Ser38, Ser65, and Ser67 resulting in all possible one-chain mutants and a no-chain mutant. Turkey satellite cells were transfected with the wild-type syndecan-4, one-chain mutants, no-chain mutant, or the empty vector and assayed for cell proliferation, differentiation, and FGF2 responsiveness. The wild-type syndecan-4, one-chain mutants, and no-chain mutant inhibited cell proliferation and delayed initial differentiation but did not alter FGF2 responsiveness or function through the mitogen-activated protein kinase pathway. There was no difference between the wild-type syndecan-4, one-chain, and no-chain mutants during these stages. These data suggest that syndecan-4 functions in an FGF2-independent manner and the GAG chains attached to the syndecan-4 core protein are not required for syndecan-4 to affect turkey satellite cell proliferation and the initial differentiation.

Authors+Show Affiliations

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, OH 44691, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18602469

Citation

Zhang, Xiangfeng, et al. "The Role of Syndecan-4 and Attached Glycosaminoglycan Chains On Myogenic Satellite Cell Growth." Matrix Biology : Journal of the International Society for Matrix Biology, vol. 27, no. 7, 2008, pp. 619-30.
Zhang X, Nestor KE, McFarland DC, et al. The role of syndecan-4 and attached glycosaminoglycan chains on myogenic satellite cell growth. Matrix Biol. 2008;27(7):619-30.
Zhang, X., Nestor, K. E., McFarland, D. C., & Velleman, S. G. (2008). The role of syndecan-4 and attached glycosaminoglycan chains on myogenic satellite cell growth. Matrix Biology : Journal of the International Society for Matrix Biology, 27(7), 619-30. https://doi.org/10.1016/j.matbio.2008.06.002
Zhang X, et al. The Role of Syndecan-4 and Attached Glycosaminoglycan Chains On Myogenic Satellite Cell Growth. Matrix Biol. 2008;27(7):619-30. PubMed PMID: 18602469.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of syndecan-4 and attached glycosaminoglycan chains on myogenic satellite cell growth. AU - Zhang,Xiangfeng, AU - Nestor,Karl E, AU - McFarland,Douglas C, AU - Velleman,Sandra G, Y1 - 2008/06/17/ PY - 2008/01/09/received PY - 2008/05/15/revised PY - 2008/06/02/accepted PY - 2008/7/8/pubmed PY - 2008/12/20/medline PY - 2008/7/8/entrez SP - 619 EP - 30 JF - Matrix biology : journal of the International Society for Matrix Biology JO - Matrix Biol VL - 27 IS - 7 N2 - The syndecans are a family of cell-surface heparan sulfate proteoglycans consisting of a core protein with covalently attached glycosaminoglycan (GAG) chains. Syndecan-4 expression in skeletal muscle is increased in growth-selected animals during proliferation. Previous studies have suggested that cell-surface heparan sulfate proteoglycans like syndecan-4 are involved in fibroblast growth factor 2 (FGF2) signaling by FGF2 binding to the heparan sulfate chains. Fibroblast growth factor 2 is a potent stimulator of muscle proliferation and an intense inhibitor of differentiation. To investigate the functional contribution of the attached syndecan-4 GAG chains, a turkey syndecan-4 full length cDNA was cloned and mutated at two or all three potential GAG attachment sites at Ser38, Ser65, and Ser67 resulting in all possible one-chain mutants and a no-chain mutant. Turkey satellite cells were transfected with the wild-type syndecan-4, one-chain mutants, no-chain mutant, or the empty vector and assayed for cell proliferation, differentiation, and FGF2 responsiveness. The wild-type syndecan-4, one-chain mutants, and no-chain mutant inhibited cell proliferation and delayed initial differentiation but did not alter FGF2 responsiveness or function through the mitogen-activated protein kinase pathway. There was no difference between the wild-type syndecan-4, one-chain, and no-chain mutants during these stages. These data suggest that syndecan-4 functions in an FGF2-independent manner and the GAG chains attached to the syndecan-4 core protein are not required for syndecan-4 to affect turkey satellite cell proliferation and the initial differentiation. SN - 0945-053X UR - https://www.unboundmedicine.com/medline/citation/18602469/The_role_of_syndecan_4_and_attached_glycosaminoglycan_chains_on_myogenic_satellite_cell_growth_ DB - PRIME DP - Unbound Medicine ER -