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Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
J Nutr Biochem. 2009 Apr; 20(4):254-60.JN

Abstract

This study investigated the effects of fish oil on adhesion molecule expression and tissue myeloperoxidase (MPO) activity in hypercholesterolemic mice with sepsis. There were one control and two experimental groups in this study. The control group (C) was fed a regular chow diet for 7 weeks, while hypercholesterolemia in the experimental group was induced by feeding a high-fat diet (20%, w/w) with cholesterol (2%, w/w) for 4 weeks. Then the experimental group was divided into two subgroups with identical nutrient distributions except that one subgroup was fed soybean oil (SO), while part of the soybean oil was replaced by fish oil (FO) in the other one for 3 weeks. After feeding the diets for 7 weeks, sepsis was induced in all three groups by cecal and ligation and puncture (CLP), and mice were sacrificed at 0, 6 or 24 h after CLP, respectively. The results showed that the FO group had a higher intracellular interferon-gamma/interleukin-4 ratio and lower tumor necrosis factor-alpha and monocyte chemoattractant protein-1 concentrations in peritoneal lavage fluid at 6 h after CLP than those in the C and SO groups. Lymphocyte CD11a/CD18 expressions were higher at 0 and 6 h and neutrophil CD11b/CD18 were higher at 6 h in the SO group than in the FO and C groups. The SO group had higher plasma intercellular adhesion molecule (ICAM)-1 levels than C group at 0 and 6 h, whereas no difference in ICAM-1 concentrations were observed between the C and FO groups at 0 h after CLP. Hypercholesterolemia resulted in higher tissue MPO activities. There were no differences in MPO activities in various organs between the two experimental groups. These results suggest that hypercholesterolemic mice fed FO did not exhibit immunosuppression when complicated with sepsis. FO administration reduced adhesion molecule expressions and inflammatory-related mediators at the site of injury at an early but not a late stage of sepsis. However, compared with the SO group, the influences of FO on MPO activities in various organs were not obvious in hypercholesterolemic mice with sepsis.

Authors+Show Affiliations

Graduate Institute of Pharmacy, Taipei Medical University, Taipei 110, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18602812

Citation

Chiu, Wan-Chun, et al. "Effects of Dietary Fish Oil Supplementation On Cellular Adhesion Molecule Expression and Tissue Myeloperoxidase Activity in Hypercholesterolemic Mice With Sepsis." The Journal of Nutritional Biochemistry, vol. 20, no. 4, 2009, pp. 254-60.
Chiu WC, Wang YC, Chien YW, et al. Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis. J Nutr Biochem. 2009;20(4):254-60.
Chiu, W. C., Wang, Y. C., Chien, Y. W., Hou, Y. C., Hu, Y. M., & Yeh, S. L. (2009). Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis. The Journal of Nutritional Biochemistry, 20(4), 254-60. https://doi.org/10.1016/j.jnutbio.2008.03.001
Chiu WC, et al. Effects of Dietary Fish Oil Supplementation On Cellular Adhesion Molecule Expression and Tissue Myeloperoxidase Activity in Hypercholesterolemic Mice With Sepsis. J Nutr Biochem. 2009;20(4):254-60. PubMed PMID: 18602812.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis. AU - Chiu,Wan-Chun, AU - Wang,Yao-Chang, AU - Chien,Yi-Wen, AU - Hou,Yu-Chen, AU - Hu,Ya-Mei, AU - Yeh,Sung-Ling, Y1 - 2008/07/07/ PY - 2007/10/15/received PY - 2008/02/18/revised PY - 2008/03/05/accepted PY - 2008/7/8/pubmed PY - 2009/7/25/medline PY - 2008/7/8/entrez SP - 254 EP - 60 JF - The Journal of nutritional biochemistry JO - J Nutr Biochem VL - 20 IS - 4 N2 - This study investigated the effects of fish oil on adhesion molecule expression and tissue myeloperoxidase (MPO) activity in hypercholesterolemic mice with sepsis. There were one control and two experimental groups in this study. The control group (C) was fed a regular chow diet for 7 weeks, while hypercholesterolemia in the experimental group was induced by feeding a high-fat diet (20%, w/w) with cholesterol (2%, w/w) for 4 weeks. Then the experimental group was divided into two subgroups with identical nutrient distributions except that one subgroup was fed soybean oil (SO), while part of the soybean oil was replaced by fish oil (FO) in the other one for 3 weeks. After feeding the diets for 7 weeks, sepsis was induced in all three groups by cecal and ligation and puncture (CLP), and mice were sacrificed at 0, 6 or 24 h after CLP, respectively. The results showed that the FO group had a higher intracellular interferon-gamma/interleukin-4 ratio and lower tumor necrosis factor-alpha and monocyte chemoattractant protein-1 concentrations in peritoneal lavage fluid at 6 h after CLP than those in the C and SO groups. Lymphocyte CD11a/CD18 expressions were higher at 0 and 6 h and neutrophil CD11b/CD18 were higher at 6 h in the SO group than in the FO and C groups. The SO group had higher plasma intercellular adhesion molecule (ICAM)-1 levels than C group at 0 and 6 h, whereas no difference in ICAM-1 concentrations were observed between the C and FO groups at 0 h after CLP. Hypercholesterolemia resulted in higher tissue MPO activities. There were no differences in MPO activities in various organs between the two experimental groups. These results suggest that hypercholesterolemic mice fed FO did not exhibit immunosuppression when complicated with sepsis. FO administration reduced adhesion molecule expressions and inflammatory-related mediators at the site of injury at an early but not a late stage of sepsis. However, compared with the SO group, the influences of FO on MPO activities in various organs were not obvious in hypercholesterolemic mice with sepsis. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/18602812/Effects_of_dietary_fish_oil_supplementation_on_cellular_adhesion_molecule_expression_and_tissue_myeloperoxidase_activity_in_hypercholesterolemic_mice_with_sepsis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(08)00078-8 DB - PRIME DP - Unbound Medicine ER -