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Cigarette smoke induced oxidative insult in local population of Pokhara.
Kathmandu Univ Med J (KUMJ). 2007 Oct-Dec; 5(4):511-7.KU

Abstract

OBJECTIVES

To assess the effect of cigarette smoking on lipid peroxidation induced oxidative stress, antioxidants, uric acid and blood sugar in normal subjects.

METHODS

The study included 61 normal subjects with regular smoking habit and 57 never-smokers normal subjects matched in respect to socio-economic status, age and BMI. Information regarding smoking habit and other personal details were collected by oral questionnaire. Total antioxidant activity (TAA), reduced glutathione (GSH), alpha-tocopherol (alpha-T), ascorbic acid (AA), uric acid (UA), plasma and urinary thiobarbituric acid reactive substances (TBARS), fasting blood sugar (FBS) and urinary creatinine (Cr) were estimated by standard procedures in both the groups. Ferric Reducing Antioxidant Power (FRAP) procedure is used to estimate TAA which measures total dietary antioxidants. Statistical analysis was done with SPSS version 10.

RESULTS

The mean pack years smoked by smokers was 14.4 +/- 15.8. The plasma TBARS level in smokers and never-smokers was 2.6 +/- 0.8 and 2.5 +/- 0.6 micromol/L respectively. The respective figure for urinary TBARS level was 4.6 +/- 2.7 and 3.7 +/- 1.4 micromol/gmCr. Smokers did not show any significant difference from never-smokers with respect to GSH, alpha-T, AA, plasma TBARS and FBS. However, the smokers had significantly lower levels of TAA (p<0.05) and raised level of urinary TBARS (p<0.05) and uric acid (p<0.01) as compared to never-smokers.

CONCLUSION

Our study suggests that smoking induces mild lipid peroxidation but the body is able to compensate for it by removing its adducts. Importantly it also indicates enhanced oxidation of purines which are essential components of both DNA and RNA. Dietary antioxidants are consumed to scavenge free radicals (FR) and other reactive species (RS) in smoke. Female smokers are more prone to oxidative insult than male smokers. In summary RS present in smoke induce mild lipid peroxidation but are not the major contributors of redox imbalance in smoke induced toxicity in the selected subjects.

Authors+Show Affiliations

Department of Biochemistry, Manipal College of Medical Sciences, Pokhara, Nepal. jayjha45@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18604085

Citation

Jha, J C., et al. "Cigarette Smoke Induced Oxidative Insult in Local Population of Pokhara." Kathmandu University Medical Journal (KUMJ), vol. 5, no. 4, 2007, pp. 511-7.
Jha JC, Maharjan BR, Adhikari D, et al. Cigarette smoke induced oxidative insult in local population of Pokhara. Kathmandu Univ Med J (KUMJ). 2007;5(4):511-7.
Jha, J. C., Maharjan, B. R., Adhikari, D., Vishwanath, P., Akila, ., Nagamma, T., Azhari, S., & Singh, P. P. (2007). Cigarette smoke induced oxidative insult in local population of Pokhara. Kathmandu University Medical Journal (KUMJ), 5(4), 511-7.
Jha JC, et al. Cigarette Smoke Induced Oxidative Insult in Local Population of Pokhara. Kathmandu Univ Med J (KUMJ). 2007 Oct-Dec;5(4):511-7. PubMed PMID: 18604085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cigarette smoke induced oxidative insult in local population of Pokhara. AU - Jha,J C, AU - Maharjan,B R, AU - Adhikari,D, AU - Vishwanath,P, AU - Akila,, AU - Nagamma,T, AU - Azhari,S, AU - Singh,P P, PY - 2008/7/8/pubmed PY - 2008/12/17/medline PY - 2008/7/8/entrez SP - 511 EP - 7 JF - Kathmandu University medical journal (KUMJ) JO - Kathmandu Univ Med J (KUMJ) VL - 5 IS - 4 N2 - OBJECTIVES: To assess the effect of cigarette smoking on lipid peroxidation induced oxidative stress, antioxidants, uric acid and blood sugar in normal subjects. METHODS: The study included 61 normal subjects with regular smoking habit and 57 never-smokers normal subjects matched in respect to socio-economic status, age and BMI. Information regarding smoking habit and other personal details were collected by oral questionnaire. Total antioxidant activity (TAA), reduced glutathione (GSH), alpha-tocopherol (alpha-T), ascorbic acid (AA), uric acid (UA), plasma and urinary thiobarbituric acid reactive substances (TBARS), fasting blood sugar (FBS) and urinary creatinine (Cr) were estimated by standard procedures in both the groups. Ferric Reducing Antioxidant Power (FRAP) procedure is used to estimate TAA which measures total dietary antioxidants. Statistical analysis was done with SPSS version 10. RESULTS: The mean pack years smoked by smokers was 14.4 +/- 15.8. The plasma TBARS level in smokers and never-smokers was 2.6 +/- 0.8 and 2.5 +/- 0.6 micromol/L respectively. The respective figure for urinary TBARS level was 4.6 +/- 2.7 and 3.7 +/- 1.4 micromol/gmCr. Smokers did not show any significant difference from never-smokers with respect to GSH, alpha-T, AA, plasma TBARS and FBS. However, the smokers had significantly lower levels of TAA (p<0.05) and raised level of urinary TBARS (p<0.05) and uric acid (p<0.01) as compared to never-smokers. CONCLUSION: Our study suggests that smoking induces mild lipid peroxidation but the body is able to compensate for it by removing its adducts. Importantly it also indicates enhanced oxidation of purines which are essential components of both DNA and RNA. Dietary antioxidants are consumed to scavenge free radicals (FR) and other reactive species (RS) in smoke. Female smokers are more prone to oxidative insult than male smokers. In summary RS present in smoke induce mild lipid peroxidation but are not the major contributors of redox imbalance in smoke induced toxicity in the selected subjects. SN - 1812-2078 UR - https://www.unboundmedicine.com/medline/citation/18604085/Cigarette_smoke_induced_oxidative_insult_in_local_population_of_Pokhara_ L2 - https://antibodies.cancer.gov/detail/CPTC-MTOR-8 DB - PRIME DP - Unbound Medicine ER -