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Two-dimensional liquid chromatography coupled with mass spectrometry for the analysis of Lobelia chinensis Lour. using an ESI/APCI multimode ion source.
J Sep Sci 2008; 31(13):2388-94JS

Abstract

A comprehensive approach for the separation and identification of components in a traditional Chinese medicine Lobelia chinensis Lour. was developed using 2D-HPLC coupled with an online photodiode array (PDA) detector and a mass spectrometer. The extract of L. chinensis Lour. was separated on a CN column in the first-dimensional HPLC, and then each of the collected fractions was further separated on an ODS column followed by an online PDA detector. After separation in the two different chromatographic modes, the eluents were delivered to a quadrupole mass spectrometer equipped with a multimode ion source of an ESI and an atmospheric pressure chemical ionization (ESI/APCI). At least 536 components in L. chinensis Lour. extract were detected and 6 of them were identified as apigenin 7-O-rutinoside, luteolin, lobetyolinin, lobetyolin, diosmin, and linarin, respectively, according to their UV spectrum and mass spectrum. The results demonstrated the powerful resolution, high peak capacity, as well as the identification capability of the 2D-HPLC combined with PDA and ESI/APCI-MS for the analyses of complex samples.

Authors+Show Affiliations

School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18604839

Citation

Zhou, Ying, et al. "Two-dimensional Liquid Chromatography Coupled With Mass Spectrometry for the Analysis of Lobelia Chinensis Lour. Using an ESI/APCI Multimode Ion Source." Journal of Separation Science, vol. 31, no. 13, 2008, pp. 2388-94.
Zhou Y, Wang Y, Wang R, et al. Two-dimensional liquid chromatography coupled with mass spectrometry for the analysis of Lobelia chinensis Lour. using an ESI/APCI multimode ion source. J Sep Sci. 2008;31(13):2388-94.
Zhou, Y., Wang, Y., Wang, R., Guo, F., & Yan, C. (2008). Two-dimensional liquid chromatography coupled with mass spectrometry for the analysis of Lobelia chinensis Lour. using an ESI/APCI multimode ion source. Journal of Separation Science, 31(13), pp. 2388-94. doi:10.1002/jssc.200700685.
Zhou Y, et al. Two-dimensional Liquid Chromatography Coupled With Mass Spectrometry for the Analysis of Lobelia Chinensis Lour. Using an ESI/APCI Multimode Ion Source. J Sep Sci. 2008;31(13):2388-94. PubMed PMID: 18604839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two-dimensional liquid chromatography coupled with mass spectrometry for the analysis of Lobelia chinensis Lour. using an ESI/APCI multimode ion source. AU - Zhou,Ying, AU - Wang,Yan, AU - Wang,Renfeng, AU - Guo,Fei, AU - Yan,Chao, PY - 2008/7/8/pubmed PY - 2008/10/10/medline PY - 2008/7/8/entrez SP - 2388 EP - 94 JF - Journal of separation science JO - J Sep Sci VL - 31 IS - 13 N2 - A comprehensive approach for the separation and identification of components in a traditional Chinese medicine Lobelia chinensis Lour. was developed using 2D-HPLC coupled with an online photodiode array (PDA) detector and a mass spectrometer. The extract of L. chinensis Lour. was separated on a CN column in the first-dimensional HPLC, and then each of the collected fractions was further separated on an ODS column followed by an online PDA detector. After separation in the two different chromatographic modes, the eluents were delivered to a quadrupole mass spectrometer equipped with a multimode ion source of an ESI and an atmospheric pressure chemical ionization (ESI/APCI). At least 536 components in L. chinensis Lour. extract were detected and 6 of them were identified as apigenin 7-O-rutinoside, luteolin, lobetyolinin, lobetyolin, diosmin, and linarin, respectively, according to their UV spectrum and mass spectrum. The results demonstrated the powerful resolution, high peak capacity, as well as the identification capability of the 2D-HPLC combined with PDA and ESI/APCI-MS for the analyses of complex samples. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/18604839/Two_dimensional_liquid_chromatography_coupled_with_mass_spectrometry_for_the_analysis_of_Lobelia_chinensis_Lour__using_an_ESI/APCI_multimode_ion_source_ L2 - https://doi.org/10.1002/jssc.200700685 DB - PRIME DP - Unbound Medicine ER -