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In vitro inhibition of dengue virus entry by p-sulfoxy-cinnamic acid and structurally related combinatorial chemistries.
Antiviral Res. 2008 Nov; 80(2):135-42.AR

Abstract

The anti-adhesive compound p-sulfoxy-cinnamic acid, zosteric acid (ZA), is derived from the temperate marine eelgrass, Zostera marina. ZA and five combinatorial chemistries based on ZA were evaluated for their anti-viral properties against dengue virus in a focus forming unit reduction assay. None of the compounds showed evidence of toxicity to the monkey kidney cell line LLCMK-2 over the concentration ranges tested. ZA showed a modest IC(50) of approximately 2.3 mM against DENV-2. Three other compounds showed IC(50) values of 2.5, 2.4, 0.3 mM, with a fourth not achieving a 50% inhibitory concentration against DENV-2. The most active compound, CF 238, showed IC(50) values of 24, 46, 14 and 47 microM against DENV-1, DENV-2, DENV-3 and DENV-4, respectively. CF 238 showed evidence of inhibition at an entry step in the viral life cycle and enhanced virus:cell binding as evidenced by a quantitative RT-PCR assay system. CF 238 may promote inappropriate virus:cell attachments common to all DENV strains that interfere with receptor interactions required for viral entry. These and other related chemistries may be useful as reagents for studying DENV entry, capturing and detecting DENV, and development of pharmaceuticals.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Rees CR
Biotechnology Research Group, Department of Biological Sciences, Florida Gulf Coast University, Fort Myers, FL 33965, USA. crrees@eagle.fgcu.edu
Costin JM
No affiliation info available
Fink RC
No affiliation info available
McMichael M
No affiliation info available
Fontaine KA
No affiliation info available
Isern S
No affiliation info available
Michael SF
No affiliation info available

MeSH

AnimalsAntiviral AgentsCell LineChlorocebus aethiopsCinnamatesDengueDengue VirusHumansPlant ExtractsSulfuric Acid EstersVirus InternalizationZosteraceae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18606464

Citation

Rees, Craig R., et al. "In Vitro Inhibition of Dengue Virus Entry By P-sulfoxy-cinnamic Acid and Structurally Related Combinatorial Chemistries." Antiviral Research, vol. 80, no. 2, 2008, pp. 135-42.
Rees CR, Costin JM, Fink RC, et al. In vitro inhibition of dengue virus entry by p-sulfoxy-cinnamic acid and structurally related combinatorial chemistries. Antiviral Res. 2008;80(2):135-42.
Rees, C. R., Costin, J. M., Fink, R. C., McMichael, M., Fontaine, K. A., Isern, S., & Michael, S. F. (2008). In vitro inhibition of dengue virus entry by p-sulfoxy-cinnamic acid and structurally related combinatorial chemistries. Antiviral Research, 80(2), 135-42. https://doi.org/10.1016/j.antiviral.2008.05.007
Rees CR, et al. In Vitro Inhibition of Dengue Virus Entry By P-sulfoxy-cinnamic Acid and Structurally Related Combinatorial Chemistries. Antiviral Res. 2008;80(2):135-42. PubMed PMID: 18606464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro inhibition of dengue virus entry by p-sulfoxy-cinnamic acid and structurally related combinatorial chemistries. AU - Rees,Craig R, AU - Costin,Joshua M, AU - Fink,Ryan C, AU - McMichael,Matthew, AU - Fontaine,Krystal A, AU - Isern,Sharon, AU - Michael,Scott F, Y1 - 2008/06/13/ PY - 2007/12/15/received PY - 2008/05/15/revised PY - 2008/05/17/accepted PY - 2008/7/9/pubmed PY - 2008/11/13/medline PY - 2008/7/9/entrez SP - 135 EP - 42 JF - Antiviral research JO - Antiviral Res VL - 80 IS - 2 N2 - The anti-adhesive compound p-sulfoxy-cinnamic acid, zosteric acid (ZA), is derived from the temperate marine eelgrass, Zostera marina. ZA and five combinatorial chemistries based on ZA were evaluated for their anti-viral properties against dengue virus in a focus forming unit reduction assay. None of the compounds showed evidence of toxicity to the monkey kidney cell line LLCMK-2 over the concentration ranges tested. ZA showed a modest IC(50) of approximately 2.3 mM against DENV-2. Three other compounds showed IC(50) values of 2.5, 2.4, 0.3 mM, with a fourth not achieving a 50% inhibitory concentration against DENV-2. The most active compound, CF 238, showed IC(50) values of 24, 46, 14 and 47 microM against DENV-1, DENV-2, DENV-3 and DENV-4, respectively. CF 238 showed evidence of inhibition at an entry step in the viral life cycle and enhanced virus:cell binding as evidenced by a quantitative RT-PCR assay system. CF 238 may promote inappropriate virus:cell attachments common to all DENV strains that interfere with receptor interactions required for viral entry. These and other related chemistries may be useful as reagents for studying DENV entry, capturing and detecting DENV, and development of pharmaceuticals. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/18606464/In_vitro_inhibition_of_dengue_virus_entry_by_p_sulfoxy_cinnamic_acid_and_structurally_related_combinatorial_chemistries_ DB - PRIME DP - Unbound Medicine ER -