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Method for the collection and analysis of volatile compounds in the breath.
J Chromatogr. 1991 Mar 08; 564(1):242-9.JC

Abstract

A new method is described for the collection and assay of volatile compounds in the breath. Subjects expired into a pump-assisted collecting apparatus in which the breath was drawn through a water trap and then through an adsorptive trap where the volatile compounds were captured on graphitized carbon and molecular sieve. The sample was subsequently eluted from the trap by thermal desorption, concentrated by two-stage cryofocusing, then assayed by gas chromatography with flame ionization and flame photometric detection. Several compounds were regularly observed in the breath of normal human volunteers, including peaks eluting with the same retention times as isoprene, ethanol, acetone, acetaldehyde and carbon disulfide. As a quantitative assay for endogenous isoprene in the breath, the method was sensitive, linear, accurate and reproducible. This method provided a number of advantages: the collection technique was acceptable to volunteers and could be used at sites remote from the laboratory. The automated assay allowed isoprene and several other volatile compounds in the breath to be observed consistently and with improved sensitivity.

Authors+Show Affiliations

Department of Medicine, St. Vincent's Medical Center of Richmond, Staten Island, NY 10310.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1860917

Citation

Phillips, M, and J Greenberg. "Method for the Collection and Analysis of Volatile Compounds in the Breath." Journal of Chromatography, vol. 564, no. 1, 1991, pp. 242-9.
Phillips M, Greenberg J. Method for the collection and analysis of volatile compounds in the breath. J Chromatogr. 1991;564(1):242-9.
Phillips, M., & Greenberg, J. (1991). Method for the collection and analysis of volatile compounds in the breath. Journal of Chromatography, 564(1), 242-9.
Phillips M, Greenberg J. Method for the Collection and Analysis of Volatile Compounds in the Breath. J Chromatogr. 1991 Mar 8;564(1):242-9. PubMed PMID: 1860917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Method for the collection and analysis of volatile compounds in the breath. AU - Phillips,M, AU - Greenberg,J, PY - 1991/3/8/pubmed PY - 1991/3/8/medline PY - 1991/3/8/entrez SP - 242 EP - 9 JF - Journal of chromatography JO - J Chromatogr VL - 564 IS - 1 N2 - A new method is described for the collection and assay of volatile compounds in the breath. Subjects expired into a pump-assisted collecting apparatus in which the breath was drawn through a water trap and then through an adsorptive trap where the volatile compounds were captured on graphitized carbon and molecular sieve. The sample was subsequently eluted from the trap by thermal desorption, concentrated by two-stage cryofocusing, then assayed by gas chromatography with flame ionization and flame photometric detection. Several compounds were regularly observed in the breath of normal human volunteers, including peaks eluting with the same retention times as isoprene, ethanol, acetone, acetaldehyde and carbon disulfide. As a quantitative assay for endogenous isoprene in the breath, the method was sensitive, linear, accurate and reproducible. This method provided a number of advantages: the collection technique was acceptable to volunteers and could be used at sites remote from the laboratory. The automated assay allowed isoprene and several other volatile compounds in the breath to be observed consistently and with improved sensitivity. UR - https://www.unboundmedicine.com/medline/citation/1860917/Method_for_the_collection_and_analysis_of_volatile_compounds_in_the_breath_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:1860917 DB - PRIME DP - Unbound Medicine ER -