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[The fabrication of hepatitis B electrochemical immunosensor array].
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008 Apr; 25(2):450-3.SW

Abstract

Based on integrate circuit (IC) technology, an eight-channel gold electrodes (GEs) array was developed. The immunosensor array is prepared by co-immobilizing thionine and Hepatitis B (HB) antibody on the gold electrodes through covalently binding them to GEs with a cysteamine/glutaraldehyde linkage. Hepatitis B surface antigen (HBsAg) was detected qualitatively and quantitatively by the peak current decrease percentage of the thionine. HBsAg positive/negative standard serum was well defined by the array. 8-channel synchronous detection for HBsAg was noted to be of good accuracy and reliability. The results of its clinical application were in good agreement with the results from ELISA.

Authors+Show Affiliations

Dai X
General Hospital of Guangzhou Command, Guangzhou 510010, China.
Liu Z
No affiliation info available

MeSH

Biosensing TechniquesElectric ImpedanceElectrochemistryElectrodesHepatitis B AntibodiesHepatitis B Surface AntigensHumansImmunoassay

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18610640

Citation

Dai, Xiaofeng, and Zhongming Liu. "[The Fabrication of Hepatitis B Electrochemical Immunosensor Array]." Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi, vol. 25, no. 2, 2008, pp. 450-3.
Dai X, Liu Z. [The fabrication of hepatitis B electrochemical immunosensor array]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008;25(2):450-3.
Dai, X., & Liu, Z. (2008). [The fabrication of hepatitis B electrochemical immunosensor array]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi, 25(2), 450-3.
Dai X, Liu Z. [The Fabrication of Hepatitis B Electrochemical Immunosensor Array]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008;25(2):450-3. PubMed PMID: 18610640.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The fabrication of hepatitis B electrochemical immunosensor array]. AU - Dai,Xiaofeng, AU - Liu,Zhongming, PY - 2008/7/10/pubmed PY - 2009/6/24/medline PY - 2008/7/10/entrez SP - 450 EP - 3 JF - Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi JO - Sheng Wu Yi Xue Gong Cheng Xue Za Zhi VL - 25 IS - 2 N2 - Based on integrate circuit (IC) technology, an eight-channel gold electrodes (GEs) array was developed. The immunosensor array is prepared by co-immobilizing thionine and Hepatitis B (HB) antibody on the gold electrodes through covalently binding them to GEs with a cysteamine/glutaraldehyde linkage. Hepatitis B surface antigen (HBsAg) was detected qualitatively and quantitatively by the peak current decrease percentage of the thionine. HBsAg positive/negative standard serum was well defined by the array. 8-channel synchronous detection for HBsAg was noted to be of good accuracy and reliability. The results of its clinical application were in good agreement with the results from ELISA. SN - 1001-5515 UR - https://www.unboundmedicine.com/medline/citation/18610640/[The_fabrication_of_hepatitis_B_electrochemical_immunosensor_array]_ L2 - http://www.diseaseinfosearch.org/result/3332 DB - PRIME DP - Unbound Medicine ER -