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Comparison of a slide agglutination test, LeptoTek Dri-Dot, and IgM-ELISA with microscopic agglutination test for Leptospira antibody detection.
Southeast Asian J Trop Med Public Health. 2007 Nov; 38(6):1111-4.SA

Abstract

A slide agglutination test (SAT), LeptoTek Dri-Dot and IgM-ELISA were compared with a microscopic agglutination test (MAT) for the detection of Leptospira antibodies. Paired sera from 10 patients whose leptospirosis was clinically suspected and diagnosed by MAT, were evaluated in this study. Our data, especially from acute samples, demonstrate the SAT and Dri-Dot were more sensitive as initial screening tests than MAT. IgM-ELISA has an advantage over MAT, SAT, and Dri-Dot since the results can be interpreted from a single serum testing if the results of the test are positive. Eight of the ten cases could be diagnosed by IgM-ELISA. Our data suggest that IgM-ELISA may be used for the diagnosis of leptospirosis. However, the agglutination test is useful for screening and for secondary infection cases for which IgM antibodies may be undetectable. MAT can be performed as a reference test and when information regarding the causative serovar is required.

Authors+Show Affiliations

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. chintana_chula@yahoo.co.thNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

18613554

Citation

Chirathaworn, Chintana, et al. "Comparison of a Slide Agglutination Test, LeptoTek Dri-Dot, and IgM-ELISA With Microscopic Agglutination Test for Leptospira Antibody Detection." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 38, no. 6, 2007, pp. 1111-4.
Chirathaworn C, Kaewopas Y, Poovorawan Y, et al. Comparison of a slide agglutination test, LeptoTek Dri-Dot, and IgM-ELISA with microscopic agglutination test for Leptospira antibody detection. Southeast Asian J Trop Med Public Health. 2007;38(6):1111-4.
Chirathaworn, C., Kaewopas, Y., Poovorawan, Y., & Suwancharoen, D. (2007). Comparison of a slide agglutination test, LeptoTek Dri-Dot, and IgM-ELISA with microscopic agglutination test for Leptospira antibody detection. The Southeast Asian Journal of Tropical Medicine and Public Health, 38(6), 1111-4.
Chirathaworn C, et al. Comparison of a Slide Agglutination Test, LeptoTek Dri-Dot, and IgM-ELISA With Microscopic Agglutination Test for Leptospira Antibody Detection. Southeast Asian J Trop Med Public Health. 2007;38(6):1111-4. PubMed PMID: 18613554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of a slide agglutination test, LeptoTek Dri-Dot, and IgM-ELISA with microscopic agglutination test for Leptospira antibody detection. AU - Chirathaworn,Chintana, AU - Kaewopas,Yada, AU - Poovorawan,Yong, AU - Suwancharoen,Duangjai, PY - 2008/7/11/pubmed PY - 2008/12/17/medline PY - 2008/7/11/entrez SP - 1111 EP - 4 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 38 IS - 6 N2 - A slide agglutination test (SAT), LeptoTek Dri-Dot and IgM-ELISA were compared with a microscopic agglutination test (MAT) for the detection of Leptospira antibodies. Paired sera from 10 patients whose leptospirosis was clinically suspected and diagnosed by MAT, were evaluated in this study. Our data, especially from acute samples, demonstrate the SAT and Dri-Dot were more sensitive as initial screening tests than MAT. IgM-ELISA has an advantage over MAT, SAT, and Dri-Dot since the results can be interpreted from a single serum testing if the results of the test are positive. Eight of the ten cases could be diagnosed by IgM-ELISA. Our data suggest that IgM-ELISA may be used for the diagnosis of leptospirosis. However, the agglutination test is useful for screening and for secondary infection cases for which IgM antibodies may be undetectable. MAT can be performed as a reference test and when information regarding the causative serovar is required. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/18613554/Comparison_of_a_slide_agglutination_test_LeptoTek_Dri_Dot_and_IgM_ELISA_with_microscopic_agglutination_test_for_Leptospira_antibody_detection_ L2 - https://antibodies.cancer.gov/detail/CPTC-SAT1-1 DB - PRIME DP - Unbound Medicine ER -